Transcriptional and translational mechanisms contribute to regulate the expression of Disc Large 1 protein during different biological processes
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De Gruyter
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Human discs large (DLG1) has been demonstrated
to be involved in cell polarity and maintenance of tissue
architecture. However, the mechanisms controlling DLG1
expression are not fully understood. This is relevant as
DLG1 is lost during the later stages of malignant progression. We initiated a series of studies to analyse the mechanisms regulating DLG1 expression. We have previously
reported the identification of an alternative splicing event
in the 5′ untranslated region (5′ -UTR) of DLG1 mRNA that
generates transcripts with two different 5′ -UTR (short and
large 5′ -UTR variants). In this study, we further examined
the impact of the DLG1 transcription and the role of the
differential expression of the alternative 5′ -UTRs on DLG1
protein levels. We analysed these mechanisms during cell
processes like differentiation, cell cycle progression and
cell-cell contact formation, where the importance of DLG1
activities was previously established. The data presented
in this report suggest that the transcriptional regulation
of DLG1 strongly contributes to DLG1 abundance and that
differential expression of alternative 5′ -UTRs with different translational properties, also cooperates, depending
on the cell type and cell situation. This study provides
new evidence for understanding the transcriptional regulation of DLG1 and the changes in DLG1 expression during
different biological processes
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