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  • ÍtemAcceso Abierto
    Probiotic properties of Bacillus subtilis DG101 isolated from the traditional Japanese fermented food nattō
    (Frontiers, 2023-09-28) Leñini, Cecilia; Rodriguez Ayala, Facundo; Goñi, Anibal Juan; Rateni, Liliana; Grau, Roberto Ricardo; Nakamura, Akira
    Spore-forming probiotic bacteria offer interesting properties as they have an intrinsic high stability, and when consumed, they are able to survive the adverse conditions encountered during the transit thorough the host gastrointestinal (GI) tract. A traditional healthy food, nattō, exists in Japan consisting of soy fermented by the spore-forming bacterium Bacillus subtilis natto. The consumption of nattō is linked to many beneficial health effects, including the prevention of high blood pressure, osteoporosis, and cardiovascular-associated disease. We hypothesize that the bacterium B. subtilis natto plays a key role in the beneficial effects of nattō for humans. Here, we present the isolation of B. subtilis DG101 from nattō and its characterization as a novel spore-forming probiotic strain for human consumption. B. subtilis DG101 was non-hemolytic and showed high tolerance to lysozyme, low pH, bile salts, and a strong adherence ability to extracellular matrix proteins (i.e., fibronectin and collagen), demonstrating its potential application for competitive exclusion of pathogens. B. subtilis DG101 forms robust liquid and solid biofilms and expresses several extracellular enzymes with activity against food diet-associated macromolecules (i.e., proteins, lipids, and polysaccharides) that would be important to improve food diet digestion by the host. B. subtilis DG101 was able to grow in the presence of toxic metals (i.e., chromium, cadmium, and arsenic) and decreased their bioavailability, a feature that points to this probiotic as an interesting agent for bioremediation in cases of food and water poisoning with metals. In addition, B. subtilis DG101 was sensitive to antibiotics commonly used to treat infections in medical settings, and at the same time, it showed a potent antimicrobial effect against pathogenic bacteria and fungi. In mammalians (i.e., rats), B. subtilis DG101 colonized the GI tract, and improved the lipid and protein serum homeostasis of animals fed on the base of a normal- or a deficient-diet regime (dietary restriction). In the animal model for longevity studies, Caenorhabditis elegans, B. subtilis DG101 significantly increased the animal lifespan and prevented its age-related behavioral decay. Overall, these results demonstrate that B. subtilis DG101 is the key component of nattō with interesting probiotic properties to improve and protect human health.
  • ÍtemAcceso Abierto
    Factors associated with mortality among hospitalized patients with COVID-19 disease treated with convalescent plasma
    (American Society for Microbiology, 2023-11-08) Perichon, Armando M.; Acosta, Andrea; Di Tulio, Liliana; Munuce, María José; Pezzotto, Stella; Bottasso, Oscar; http://orcid.org/0000-0002-8472-7965
    The use of convalescent plasma (CP) for hospitalized patients with SARSCoV-2 infection might be a useful option in certain settings. Soon after the outbreak of COVID-19, the National Ministry of Health of Argentina recommended the use of CP transfusion for hospitalized patients with COVID-19 disease. Between 1 June and 3 October 2020, 480 patients, excluding those on invasive mechanical ventilation (IMV), received at least one CP infusion in the province of Santa Fe. We aimed to find factors associated with mortality among this cohort of patients. The median age was 60 years (interquartile range: 49–69 years) and 320 (66.7%) were males. Most of these patients (93.75%) received a single CP infusion, 82.1% and 95.6% before day 4 and day 7 of hospitalization, respectively. Anti-SARS-CoV-2 titers were determined in the CP units administered using Elecsys Anti-SARS-CoV-2 S assay. At 28 days of follow-up, 250 patients were discharged (52.1%), 131 (27.3%) remained hospitalized without and 16 (3.3%) with oxygen requirement, 27 (5.6%) were on IMV, and 56 (11.7%) had died. In the multivariate logistic regression analysis, the factors significantly associated with 28-day mortality were (i) requirement of IMV, (ii) the administration of CP after the third day of hospitalization, (iii) age, and (iv) number of comorbidities. The qualitative and quantitative analyses of antibodies against SARS-CoV-2 in the infused CP were not associated with mortality. Our findings may imply a seemingly favorable effect of CP administration among patients with severe COVID-19 disease when infused sooner after hospitalization.
  • ÍtemAcceso Abierto
    The lambda variant in Argentina: analyzing the evolution and spread of SARS-CoV-2 lineage C.37
    (MDPI, 2023-06-16) Nabaes Jodar, Mercedes; Torres, Carolina; Mojsiejczuk, Laura; Acuña, Dolores; Valinotto, Laura; Goya, Stephanie; Natale, Monica; Alexay, Sofia; Amadio, Ariel Fernando; Irazoqui, José Matías; Fernández, Franco; Acevedo, Maria Elina; Alvarez Lopez, Cristina; Angelletti, Andres; Aulicino, Paula; Bolatti, Elisa María; Brusés, Bettina; Cacciabue, Marco; Cavatorta, Ana Laura; Cerri, Agustina; Cordero, Andres; Debat, Humberto; Dus Santos, María José; Eberhardt, María Florencia; Ercole, Regina; Espul, Carlos; Farber, Marisa; Fay, Fabián; Fernández, María Ailén; Ferrini, Florencia; Formichelli, Laura; Ceballos, Santiago; Gallego, Fernando; Giri, Adriana Angélica; Gismondi, María Ines; Acevedo, Raul Maximiliano; Gramundi, Iván; Ibañez, María Eugenia; König, Guido Alberto; Leiva, Viviana; Lorenzini Campos, Melina; Lucero, Horacio; Marquez, Nathalie; Mazzeo, Melina; Mistchenko, Alicia Susana; Montoto, Luciana; Muñoz, Marianne; Nadalich, Victoria; Nardi, Cristina; Ortiz, Belén; Pianciola, Luis; Pintos, Carolina Beatriz; Puebla, Andrea Fabiana; Rastellini, Carolina Victoria; Rojas, Alejandro Ezequiel; Sfalcin, Javier; Suárez, Ariel; Theaux, Clara; Thomas, Guillermo; Tittarelli, Estefanía; Toro, Rosana; Villanova, Gabriela Vanina; Wenk, Gretel; Ziehm, María Cecilia; Zimmermann, María Carla; Zimmermann, María Carla; Proyecto PAIS Working Group; Viegas, Mariana
  • ÍtemAcceso Abierto
    Longitudinal follow-up of the immunity to SARS-CoV-2 in health care workers in Argentina: Persistence of humoral response and neutralizing capacity after Sputnik V vaccination
    (NLM (Medline), 2023-06-22) Castro, Eliana F.; Acosta, Julián; Moriena, Lucía; Rios Medrano, Mayra; Tejerina Cibello, Malena; Codino, Eduardo; Taborda, Miguel Ángel; Álvarez, Diego E.; Cavatorta, Ana Laura
  • ÍtemAcceso Abierto
    Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions
    (Frontiers Media S.A., 2023-02-08) Acciarri, Giuliana; Gizzi, Fernán O.; Torres Manno, Mariano; Stülke, Jörg; Blancato, Victor; Magni, Christian
  • ÍtemAcceso Abierto
    Corrigendum: Identification of novel thermosensors in gram-positive pathogens
    (2022-08-31) Fernández, Pilar; Díaz, Alejandra Raquel; Ré, María Florencia; Porrini, Lucía; De Mendoza, Diego; Albanesi, Daniela; Mansilla, María Cecilia
  • ÍtemAcceso Abierto
    Influence of physicochemical factors on adsorption of ten Shigella flexneri Phages
    (MDPI, 2022-12-16) Tomat, David Damián; Aquili, Virginia; Casabonne, Cecilia; Quiberoni, Andrea
  • ÍtemAcceso Abierto
    Update on the biological relevance of lysine acetylation as a novel drug target in trypanosomatids
    (Bentham Science Publishers) Martinez Peralta, Gonzalo; Serra, Esteban C.; Alonso, Victoria Lucía
  • ÍtemAcceso Abierto
    In Vitro Drug Screening Against All Life Cycle Stages of Trypanosoma cruzi Using Parasites Expressing β-galactosidase
    (MyJove Corporation, 2021-11) Alonso, Victoria Lucía; Manarin, Romina; Perdomo, Virginia Gabriela; Gulin, Julián Ernesto Nicolás; Serra, Esteban C.; Cribb, Pamela
    Trypanosoma cruzi is the causative agent of Chagas disease (ChD), an endemic disease of public health importance in Latin America that also affects many non-endemic countries due to the increase in migration. This disease affects nearly 8 million people, with new cases estimated at 50,000 per year. In the 1960s and 70s, two drugs for ChD treatment were introduced: nifurtimox and benznidazole (BZN). Both are effective in newborns and during the acute phase of the disease but not in the chronic phase, and their use is associated with important side effects. These facts underscore the urgent need to intensify the search for new drugs against T. cruzi. T. cruzi is transmitted through hematophagous insect vectors of the Reduviidae and Hemiptera families. Once in the mammalian host, it multiplies intracellularly as the non-flagellated amastigote form and differentiates into the trypomastigote, the bloodstream non-replicative infective form. Inside the insect vector, trypomastigotes transform into the epimastigote stage and multiply through binary fission. This paper describes an assay based on measuring the activity of the cytoplasmic β-galactosidase released into the culture due to parasites lysis by using the substrate, chlorophenol red β-D-galactopyranoside (CPRG). For this, the T. cruzi Dm28c strain was transfected with a β-galactosidase-overexpressing plasmid and used for in vitro pharmacological screening in epimastigote, trypomastigote, and amastigote stages. This paper also describes how to measure the enzymatic activity in cultured epimastigotes, infected Vero cells with amastigotes, and trypomastigotes released from the cultured cells using the reference drug, benznidazole, as an example. This colorimetric assay is easily performed and can be scaled to a high-throughput format and applied to other T. cruzi strains.
  • ÍtemAcceso Abierto
    A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix
    (2022-10) García, Lucila; Molina, María Celeste; Padgett Pagliai, Kaylie Allyson; Torres, Pablo S.; Bruna, Roberto Emanuel; García Véscovi, Eleonora; González, Claudio F.; Gadea, José; Marano, María Rosa
  • ÍtemAcceso Abierto
    A novel Tetrahymena thermophila sterol C-22 desaturase belongs to the fatty acid hydroxylase/desaturase superfamily
    (Elsevier, 2022-10) Sanchez Granel, María L.; Siburu, Nicolás G.; Fricska, Annamária; Maldonado, Lucas L.; Gargiulo, Laura B.; Nudel, Clara B.; Uttaro, Antonio Domingo; Nusblat, Alejandro D.
  • ÍtemAcceso Abierto
    Novel effect of the high risk-HPV E7 CKII phospho-acceptor site on polarity protein expression
    (BMC, 2022-12) Dizanzo, María Paula; Bugnon Valdano, Marina Paula; Basukala, Om; Banks, Lawrence; Gardiol, Daniela
  • ÍtemAcceso Abierto
    Evolution of Copper Homeostasis and Virulence in Salmonella
    (Frontiers Media, 2022-03-16) Méndez, Andrea A. E.; Mendoza, Julián I.; Echarren, María Laura; Terán, Ignacio; Checa, Susana Karina; Soncini, Fernando C.
  • ÍtemAcceso Abierto
    Pseudomonas putida group species as reservoirs of mobilizable Tn402-like class 1 integrons carrying blaVIM-2 metallo-β-lactamase genes
    (Elsevier B.V., 2021-11-06) Brovedan, Marco Alcides; Marchiaro, Patricia M.; Díaz, María S.; Faccone, Diego; Corso, Alejandra; Pasteran, Fernando; Viale, Alejandro M.; Limansky, Adriana S.
  • ÍtemAcceso Abierto
    Cost-effective method to perform SARS-CoV-2 variant surveillance: detection of Alpha, Gamma, Lambda, Delta, Epsilon, and Zeta in Argentina
    (Frontiers Media, 2021-12-10) Proyecto Argentino Interinstitucional de genómica de SARS-CoV-2; Posner, Victoria María; Villanova, Gabriela Vanina; Acosta, Julián; Cavatorta, Ana Laura
  • ÍtemAcceso Abierto
    Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses
    (MDPI, 2022-01-24) Bolatti, Elisa María; Viarengo, Gastón; Zorec, Tomaž M; Cerri, Agustina; Montani, María E.; Hošnjak, Lea; Casal, Pablo E.; Bortolotto, Eugenia; Di Domenica, Violeta; Chouhy, Diego; Allasia, María Belén; Barquez, Rubén M.; Poljak, Mario; Giri, Adriana Angélica
  • ÍtemAcceso Abierto
    The distinctive roles played by the superoxide dismutases of the extremophile Acinetobacter sp. Ver3
    (Nature Research, 2022-03-12) Steimbrüch, Bruno A.; Sartorio, Mariana Gabriela; Cortez, Néstor; Albanesi, Daniela; Lisa, María Natalia; Repizo, Guillermo Daniel
  • ÍtemAcceso Abierto
    Phagocytic and pinocytic uptake of cholesterol in Tetrahymena thermophila impact differently on gene regulation for sterol homeostasis
    (Nature Research, 2021-04-27) Hernández, Josefina; Gabrielli, Matías; Costa, Joaquín; Uttaro, Antonio Domingo
  • ÍtemAcceso Abierto
    Identification of Novel Thermosensors in Gram-Positive Pathogens
    (Frontiers Media, 2020-11-26) Fernández, Pilar; Díaz, Alejandra Raquel; Ré, María Florencia; Porrini, Lucía; De Mendoza, Diego; Albanesi, Daniela; Mansilla, María Cecilia
  • ÍtemAcceso Abierto
    Optimization and biological validation of an in vitro assay using the transfected Dm28c/pLacZ Trypanosoma cruzi strain
    (Oxford University Press, 2021-07-13) Gulin, Julián Ernesto Nicolás; Rocco, Daniela Marisa; Alonso, Victoria Lucía; Cribb, Pamela; Altcheh, Jaime; García-Bournissen, Facundo