Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes

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dc.creator	Bugnon Valdano, Marina Paula
dc.creator	Cavatorta, Ana Laura
dc.creator	Morale, Miriam
dc.creator	Marziali, Federico Emanuel
dc.creator	Steenbergen, Renske
dc.creator	Boccardo, Enrique
dc.creator	Gardiol, Daniela
dc.date.accessioned	2018-03-08T13:53:43Z
dc.date.available	2018-03-08T13:53:43Z
dc.date.issued	2016-02
dc.identifier.issn	0022-1317
dc.identifier.uri	http://hdl.handle.net/2133/10755
dc.description	Loss of cell polarity is a fundamental process in cell transformation. Among polarity proteins, we focused on human Disc Large (DLG1), which is localized mainly at adherens junctions and contributes to the control of cell proliferation. We previously demonstrated that its expression is altered in HPV-associated cervical neoplastic lesions, but the mechanisms beyond this remain unknown. In this study, we analyzed the contribution of HPV proteins to the changes in DLG1 expression in the squamous epithelium. We observed tissue and intracellular misdistribution of DLG1 when high-risk HPV-18 E7 or E6/E7 proteins were expressed in organotypic raft cultures. The viral oncoproteins induce the loss of DLG1 from the cell borders and an increase in the level of DLG1 protein, reflecting the pattern observed in cervical lesions. These findings were corroborated in cultures bearing the entire HPV-18 genome. Interestingly, changes in tissue distribution and abundance of DLG1 were also detected in organotypic cultures expressing the low-risk HPV-11 E7 or E6/E7 proteins; suggesting a conserved function among different HPV types. However, for low-risk HPVs, the subcellular localization of DLG1 at cell-to-cell contacts was predominantly maintained. This report offers new evidence of the involvement of HPV proteins in DLG1 expression pattern and our data support previous observations regarding DLG1 expression in cervical lesions.	es
dc.description.sponsorship	International Collaborative Research grant, FAPESP (no. 2012/51017-8)/ CONICET (Res. 0990/2013)	es
dc.description.sponsorship	Minsterio de Salud de la República Argentina. Instituto Nacional del Cáncer	es
dc.description.sponsorship	Provincia de Santa Fe (Argentina), no. 2010-178-13	es
dc.description.sponsorship	Fundación Bunge Born (Oster Fellowship)	es
dc.description.sponsorship	FAPESP (2011/14416-9)	es
dc.description.sponsorship	CNPq (573799/2008-3)	es
dc.format	application/octet-stream
dc.format.extent	453-462
dc.language.iso	eng	es
dc.publisher	Microbiology Society	es
dc.rights	openAccess	es
dc.subject	HPV	es
dc.subject	DLG1	es
dc.subject	Organotypic Culture	es
dc.title	Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes	es
dc.rights.holder	Universidad Nacional de Rosario	es
dc.rights.holder	Microbiology Society	es
dc.rights.holder	Bugnon Valdano, Marina Paula	es
dc.rights.holder	Cavatorta, Ana Laura	es
dc.rights.holder	Morale, Miriam	es
dc.rights.holder	Marziali, Federico Emanuel	es
dc.rights.holder	Steenbergen, Renske	es
dc.rights.holder	Boccardo, Enrique	es
dc.rights.holder	Gardiol, Daniela	es
dc.relation.publisherversion	http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000364#tab2	es
dc.relation.publisherversion	http://www.microbiologyresearch.org/docserver/fulltext/jgv/97/2/453_jgv000364.pdf?expires=1520515543&id=id&accname=guest&checksum=429720F44CF6301F5A659089E327B866	es
dc.relation.publisherversion	http://dx.doi.org/10.1099/jgv.0.000364	es
dc.citation.title	Journal of General Virology
dc.citation.volume	97
dc.description.fil	Fil: Bugnon Valdano, Marina Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.fil	Fil: Cavatorta, Ana Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.fil	Fil: Morale, Miriam Galliote. University of Sao Paulo. Institute of Chemistry. Deparment of Biochemistry; Brasil.
dc.description.fil	Fil: Marziali, Federico Emanuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.fil	Fil: de Souza Lino, Vanesca. University of Sao Paulo. Institute of Chemistry. Deparment of Biochemistry; Brasil.
dc.description.fil	Fil: Steenbergen, Renske. VU University Medical Center. Department of Pathology; The Netherlands.
dc.description.fil	Fil: Boccardo, Enrique. University of Sao Paulo. Institute of Chemistry. Deparment of Biochemistry; Brasil.
dc.description.fil	Fil: Boccardo, Enrique. University of Sao Paulo. Institute of Chemistry. Deparment of Biochemistry; Brasil.
dc.description.fil	Fil: Gardiol, Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.