Disc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocytes

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dc.citation.titleJournal of General Virology
dc.citation.volume97
dc.creatorBugnon Valdano, Marina Paula
dc.creatorCavatorta, Ana Laura
dc.creatorMorale, Miriam
dc.creatorMarziali, Federico Emanuel
dc.creatorSteenbergen, Renske
dc.creatorBoccardo, Enrique
dc.creatorGardiol, Daniela
dc.date.accessioned2018-03-08T13:53:43Z
dc.date.available2018-03-08T13:53:43Z
dc.date.issued2016-02
dc.descriptionLoss of cell polarity is a fundamental process in cell transformation. Among polarity proteins, we focused on human Disc Large (DLG1), which is localized mainly at adherens junctions and contributes to the control of cell proliferation. We previously demonstrated that its expression is altered in HPV-associated cervical neoplastic lesions, but the mechanisms beyond this remain unknown. In this study, we analyzed the contribution of HPV proteins to the changes in DLG1 expression in the squamous epithelium. We observed tissue and intracellular misdistribution of DLG1 when high-risk HPV-18 E7 or E6/E7 proteins were expressed in organotypic raft cultures. The viral oncoproteins induce the loss of DLG1 from the cell borders and an increase in the level of DLG1 protein, reflecting the pattern observed in cervical lesions. These findings were corroborated in cultures bearing the entire HPV-18 genome. Interestingly, changes in tissue distribution and abundance of DLG1 were also detected in organotypic cultures expressing the low-risk HPV-11 E7 or E6/E7 proteins; suggesting a conserved function among different HPV types. However, for low-risk HPVs, the subcellular localization of DLG1 at cell-to-cell contacts was predominantly maintained. This report offers new evidence of the involvement of HPV proteins in DLG1 expression pattern and our data support previous observations regarding DLG1 expression in cervical lesions.es
dc.description.filFil: Bugnon Valdano, Marina Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Cavatorta, Ana Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Morale, Miriam Galliote. University of Sao Paulo. Institute of Chemistry. Deparment of Biochemistry; Brasil.
dc.description.filFil: Marziali, Federico Emanuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: de Souza Lino, Vanesca. University of Sao Paulo. Institute of Chemistry. Deparment of Biochemistry; Brasil.
dc.description.filFil: Steenbergen, Renske. VU University Medical Center. Department of Pathology; The Netherlands.
dc.description.filFil: Boccardo, Enrique. University of Sao Paulo. Institute of Chemistry. Deparment of Biochemistry; Brasil.
dc.description.filFil: Boccardo, Enrique. University of Sao Paulo. Institute of Chemistry. Deparment of Biochemistry; Brasil.
dc.description.filFil: Gardiol, Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.sponsorshipInternational Collaborative Research grant, FAPESP (no. 2012/51017-8)/ CONICET (Res. 0990/2013)es
dc.description.sponsorshipMinsterio de Salud de la República Argentina. Instituto Nacional del Cánceres
dc.description.sponsorshipProvincia de Santa Fe (Argentina), no. 2010-178-13es
dc.description.sponsorshipFundación Bunge Born (Oster Fellowship)es
dc.description.sponsorshipFAPESP (2011/14416-9)es
dc.description.sponsorshipCNPq (573799/2008-3)es
dc.formatapplication/octet-stream
dc.format.extent453-462
dc.identifier.issn0022-1317
dc.identifier.urihttp://hdl.handle.net/2133/10755
dc.language.isoenges
dc.publisherMicrobiology Societyes
dc.relation.publisherversionhttp://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000364#tab2es
dc.relation.publisherversionhttp://www.microbiologyresearch.org/docserver/fulltext/jgv/97/2/453_jgv000364.pdf?expires=1520515543&id=id&accname=guest&checksum=429720F44CF6301F5A659089E327B866es
dc.relation.publisherversionhttp://dx.doi.org/10.1099/jgv.0.000364es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderMicrobiology Societyes
dc.rights.holderBugnon Valdano, Marina Paulaes
dc.rights.holderCavatorta, Ana Lauraes
dc.rights.holderMorale, Miriames
dc.rights.holderMarziali, Federico Emanueles
dc.rights.holderSteenbergen, Renskees
dc.rights.holderBoccardo, Enriquees
dc.rights.holderGardiol, Danielaes
dc.subjectHPVes
dc.subjectDLG1es
dc.subjectOrganotypic Culturees
dc.titleDisc Large 1 expression is altered by Human Papillomavirus E7/E6 proteins in organotypic cultures of human keratinocyteses

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(FBIOyF) Departamento de Microbiología - Artículos
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