New tools for recombinant proteinproduction in Escherichia coli:A 5-year update

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dc.citation.titleProtein Science
dc.citation.volume8
dc.contributor.orcidhttps://orcid.org/0000-0002-8313-6813
dc.contributor.orcidhttps://orcid.org/0000-0001-5776-3306
dc.creatorRosano, Germán L.
dc.creatorMorales, Enrique Salvador
dc.creatorCeccarelli, Eduardo Augusto
dc.date.accessioned2024-10-31T15:47:57Z
dc.date.available2024-10-31T15:47:57Z
dc.date.issued2019-06-01
dc.description.abstractThe production of proteins in sufficient amounts is key for their study or use asbiotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production givenits fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilitiesare continuously being improved. Also, the associated tools (such as plasmids and cultivationconditions) are subject of ongoing research to optimize product yield. In this work, we review thelatest advances in recombinant protein production in E. coli.
dc.description.filFil: Rosano, Germán L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Morales, Enrique Salvador. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ceccarelli, Eduardo Augusto. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipUniversidad Nacional de Rosario
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica: PICT2015-2955
dc.format.extent1412-1422
dc.identifier.citationRosano, German Leandro; Morales, Enrique Salvador; Ceccarelli, Eduardo Augusto; New tools for recombinant protein production in Escherichia coli : A 5‐year update; John Wiley & Sons Inc; Protein Science; 28; 8; 7-2019; 1412-1422
dc.identifier.issn1469-896X
dc.identifier.urihttps://hdl.handle.net/2133/28014
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1002/pro.3668
dc.relation.publisherversionhttps://doi.org/10.1002/pro.3668
dc.rightsopenAccess
dc.rights.holderWiley
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEscherichia coli
dc.subjectE. coli strains
dc.subjectRecombinant protein production
dc.subjectExpression plasmid
dc.subjectAffinity tags
dc.subjectFusion partners
dc.subjectInclusion bodies
dc.titleNew tools for recombinant proteinproduction in Escherichia coli:A 5-year update
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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