New tools for recombinant proteinproduction in Escherichia coli:A 5-year update

The production of proteins in sufficient amounts is key for their study or use asbiotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production givenits fast growth, easy manipulation, and cost-effectiveness. As such, its protein production capabilitiesare continuously being improved. Also, the associated tools (such as plasmids and cultivationconditions) are subject of ongoing research to optimize product yield. In this work, we review thelatest advances in recombinant protein production in E. coli.