CNBP controls transcription by unfolding DNA G-quadruplex structures

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dc.citation.titleNucleic Acids Researches
dc.citation.volume47(15)es
dc.creatorDavid, Aldana P.
dc.creatorPipier, Angélique
dc.creatorPascutti, Federico
dc.creatorBinolfi, Andrés
dc.creatorWeiner, Andrea María Julia
dc.creatorChallier, Emilse
dc.creatorHeckel, Sofía
dc.creatorCalsou, Patrick
dc.creatorGomez, Dennis
dc.creatorCalcaterra, Nora B.
dc.creatorArmas, Pablo
dc.date.accessioned2021-02-22T18:48:03Z
dc.date.available2021-02-22T18:48:03Z
dc.date.issued2019-06-20
dc.descriptionGuanine-rich DNA strands can fold into noncanonical four-stranded secondary structures named G-quadruplexes (G4). Experimental evidences suggest that G4-DNA surrounding transcription start sites act as cis-regulatory elements by either stimulating or inhibiting gene transcription. Therefore, proteins able to target and regulate specific G4 formation/unfolding are crucial for G4- mediated transcriptional control. Here we present data revealing that CNBP acts in vitro as a G4- unfolding protein over a tetramolecular G4 formed by the TG4T oligonucleotide, as well as over the G4 folded in the promoters of several oncogenes. CNBP depletion in cellulo led to a reduction in the transcription of endogenous KRAS, suggesting a regulatory role of CNBP in relieving the transcriptional abrogation due to G4 formation. CNBP activity was also assayed over the evolutionary conserved G4 enhancing the transcription of NOGGIN (NOG) developmental gene. CNBP unfolded in vitro NOG G4 and experiments performed in cellulo and in vivo in developing zebrafish showed a repressive role of CNBP on the transcription of this gene by G4 unwinding. Our results shed light on the mechanisms underlying CNBP way of action, as well as reinforce the notion about the existence and function of G4s in whole living organismses
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in Nucleic Acids Research. The final authenticated version is available online at: https://doi.org/10.1093/nar/gkz527
dc.description.filFil: David, Aldana P. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Pipier, Angélique. Equipe Labellisée Ligue Nationale contre le Cancer; France.es
dc.description.filFil: Pipier, Angélique. Universite de Toulouse. Institut de Pharmacologie; France.es
dc.description.filFil: Pascutti, Federico. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Binolfi, Andrés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Weiner, Andrea María Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Challier, Emilse. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Heckel, Sofía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Calsou, Patrick. Equipe Labellisée Ligue Nationale contre le Cancer; France.es
dc.description.filFil: Calsou, Patrick. Universite de Toulouse. Institut de Pharmacologie; France.es
dc.description.filFil: Gomez, Dennis. Equipe Labellisée Ligue Nationale contre le Cancer; France.es
dc.description.filFil: Gomez, Dennis. Universite de Toulouse. Institut de Pharmacologie; France.es
dc.description.filFil: Calcaterra, Nora B. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Armas, Pablo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2014-0741, PICT 2017-0976 y PICT 2016-0671es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): 2015-0170es
dc.description.sponsorshipECOS Sud-MinCyT: A15B02es
dc.description.sponsorshipUniversidad Nacional de Rosario (UNR): BIO322es
dc.description.sponsorshipECOS Sud-CNRS: A15B02es
dc.description.sponsorshipAgence National de la Recherche: 16-CE11-0006 G4 TopIProes
dc.description.sponsorshipInstitut de Pharmacologie et Biologie Structurale (IPBS)es
dc.formatapplication/pdf
dc.format.extent7901–7913es
dc.identifier.issn1362-4962es
dc.identifier.urihttp://hdl.handle.net/2133/19667
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.publisherversionhttps://doi.org/10.1093/nar/gkz527es
dc.relation.publisherversionhttps://academic.oup.com/nar/article/47/15/7901/5521029es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderDavid, Aldana P.es
dc.rights.holderPipier, Angéliquees
dc.rights.holderPascutti, Federicoes
dc.rights.holderBinolfi, Andréses
dc.rights.holderWeiner, Andrea María Juliaes
dc.rights.holderChallier, Emilsees
dc.rights.holderHeckel, Sofíaes
dc.rights.holderCalsou, Patrickes
dc.rights.holderGomez, Dennises
dc.rights.holderCalcaterra, Nora B.es
dc.rights.holderArmas, Pabloes
dc.rights.textAttribution-NonCommercial 4.0 International (CC BY-NC 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectCellular Nucleic Acid-Binding Protein (CNBP)es
dc.subjectTranscription Factorses
dc.subjectDNA G-quadruplex Structureses
dc.subjectGuaninees
dc.titleCNBP controls transcription by unfolding DNA G-quadruplex structureses
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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