Nos complace anunciarles que hemos actualizado con éxito el RepHip a la versión 9.3 de Dspace. El repositorio ya está disponible para consultas, pero todavía no está disponible para subir nuevo material. En caso de experimentar algún problema, por favor contactarse a rephip@unr.edu.ar..

CNBP controls transcription by unfolding DNA G-quadruplex structures

Miniatura

Archivos

163.pdf (4,59 MB)

Fecha

2019-06-20

Authors










Título de la revista

ISSN de la revista

Título del volumen

Editor

Oxford University Press

Resumen

Descripción

Guanine-rich DNA strands can fold into noncanonical four-stranded secondary structures named G-quadruplexes (G4). Experimental evidences suggest that G4-DNA surrounding transcription start sites act as cis-regulatory elements by either stimulating or inhibiting gene transcription. Therefore, proteins able to target and regulate specific G4 formation/unfolding are crucial for G4- mediated transcriptional control. Here we present data revealing that CNBP acts in vitro as a G4- unfolding protein over a tetramolecular G4 formed by the TG4T oligonucleotide, as well as over the G4 folded in the promoters of several oncogenes. CNBP depletion in cellulo led to a reduction in the transcription of endogenous KRAS, suggesting a regulatory role of CNBP in relieving the transcriptional abrogation due to G4 formation. CNBP activity was also assayed over the evolutionary conserved G4 enhancing the transcription of NOGGIN (NOG) developmental gene. CNBP unfolded in vitro NOG G4 and experiments performed in cellulo and in vivo in developing zebrafish showed a repressive role of CNBP on the transcription of this gene by G4 unwinding. Our results shed light on the mechanisms underlying CNBP way of action, as well as reinforce the notion about the existence and function of G4s in whole living organisms
This is a post-peer-review, pre-copyedit version of an article published in Nucleic Acids Research. The final authenticated version is available online at: https://doi.org/10.1093/nar/gkz527

Palabras clave

Cellular Nucleic Acid-Binding Protein (CNBP), Transcription Factors, DNA G-quadruplex Structures, Guanine

Citación

URI

http://hdl.handle.net/2133/19667

Colecciones

(FBIOyF) Departamento de Ciencias Biológicas - Artículo de Revista

Aprobación

Revisión

Complementado por

Referenciado por

Licencia Creative Commons

Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
Excepto donde se indique lo contrario, la licencia de este ítem se describe como Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)