Protein kinase C activity in boar sperm

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dc.citation.titleAndrologyes
dc.citation.volumeVolume 5, Issue 2 Pages 381–391es
dc.creatorTeijeiro, Juan Manuel
dc.creatorMarini, Patricia Estela
dc.creatorBragado, María Julia
dc.creatorGarcía-Marin, Luis
dc.date.accessioned2017-11-17T01:26:06Z
dc.date.available2017-11-17T01:26:06Z
dc.date.issued2017-02-10
dc.descriptionMale germ cells undergo different processes within the female reproductive tract to successfully fertilize the oocyte. These processes are triggered by different extracellular stimuli leading to activation of protein phosphorylation. Protein kinase C (PKC) is a key regulatory enzyme in signal transduction mechanisms involved in many cellular processes. Studies in boar sperm demonstrated a role for PKC in the intracellular signaling involved in motility and cellular volume regulation. Experiments using phorbol 12-myristate 13-acetate (PMA) showed increases in the Serine/Threonine phosphorylation of substrates downstream of PKC in boar sperm. In order to gain knowledge about those cellular processes regulated by PKC, we evaluate the effects of PMA on boar sperm motility, lipid organization of plasma membrane, integrity of acrosome membrane and sperm agglutination. Also, we investigate the crosstalk between PKA and PKC intracellular pathways in spermatozoa from this species. The results presented here reveal a participation of PKC in sperm motility regulation and membrane fluidity changes, which is probably associated to acrosome reaction and to agglutination. Also, we show the existence of a hierarchy in the kinases pathway. Previous works on boar sperm suggest a pathway in which PKA is positioned upstream to PKC and this new results support such model.es
dc.description.filFil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva (CONICET); Argentinaes
dc.description.filFil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva (CONICET); Argentinaes
dc.description.filFil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentinaes
dc.description.filFil: Marini, Patricia Estela. Universidad Nacional de Rosario. Consejo de Investigaciones de la Universidad Nacional de Rosario; Argentinaes
dc.description.filFil: Bragado, María Julia. Universidad de Extremadura. Escuela de Medicina Veterinaria. Grupo de Investigación de Señalización Intracelular y Tecnología de la Reproducción; Españaes
dc.description.filFil: García-Marin, Luis. Universidad de Extremadura. Escuela de Medicina Veterinaria. Grupo de Investigación de Señalización Intracelular y Tecnología de la Reproducción; Españaes
dc.description.sponsorshipANPCyT-BID PICT 2012-0342es
dc.description.sponsorshipGovernment of Extremadura, Spain (JUEX-IBI13121, PCJ1008, GR10125 and GR10156)es
dc.description.sponsorshipPrograma AVE (Ayuda para Viajes al Exterior) Universidad Nacional de Rosario, 2011es
dc.formatapplication/pdf
dc.format.extentVolume 5, Issue 2 Pages 381–391es
dc.identifier.issn2047-2927es
dc.identifier.urihttp://hdl.handle.net/2133/9294
dc.language.isoenges
dc.publisherWileyes
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/andr.12312/abstractes
dc.rightsembargoedAccesses
dc.rights.holderWileyes
dc.rights.texthttps://authorservices.wiley.com/asset/photos/licensing-and-open-access-photos/eCTA_sample.pdfes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/*
dc.subjectEspermatozoides porcinoses
dc.subjectFluidez de membranaes
dc.subjectProtein quinasa Ces
dc.subjectBoar spermes
dc.subjectMembrane fluidityes
dc.subjectProtein kinase Ces
dc.titleProtein kinase C activity in boar spermes
dc.typearticle
dc.typeartículo
dc.typeacceptedVersion
dc.type.collectionarticulo
dc.type.versionacceptedVersiones

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