Interference of HTLV-1 Tax Protein with Cell Polarity Regulators: Defining the Subcellular Localization of the Tax-DLG1 Interaction
| dc.citation.title | Viruses | |
| dc.citation.volume | 9 (12) | |
| dc.creator | Marziali, Federico Emanuel | |
| dc.creator | Bugnon Valdano, Marina Paula | |
| dc.creator | Brunet Avalos, Clarise | |
| dc.creator | Moriena, Lucía | |
| dc.creator | Cavatorta, Ana Laura | |
| dc.creator | Gardiol, Daniela | |
| dc.date.accessioned | 2018-01-22T23:30:32Z | |
| dc.date.available | 2018-01-22T23:30:32Z | |
| dc.date.issued | 2017-11-23 | |
| dc.description | Human T cell leukemia virus (HTLV)-1 Tax (Tax) protein is very important in viral replication and cell transformation. Tax localizes in the nucleus and cytoplasm in association with organelles. Some activities of Tax depend on interactions with PDZ (PSD-95/Discs Large/Z0-1) domain–containing proteins such as Discs large protein 1 (DLG1) which is involved in cell polarity and proliferation. The DLG1 interaction results in a cytoplasmic co-localization pattern resembling vesicular aggregates, the nature of which is still unknown. To further explore the role of PDZ proteins in HTLV-1 cell transformation, we deeply investigated the Tax-DLG1 association. By fluorescence resonance energy transfer (FRET), we detected, for the first time, the direct binding of Tax to DLG1 within the cell. We showed that the interaction specifically affects the cellular distribution of not only DLG1, but also Tax. After studying different cell structures, we demonstrated that the aggregates distribute into the Golgi apparatus in spatial association with the microtubule-organizing center (MTOC). This study contributes to understand the biological significance of Tax-PDZ interactions | es |
| dc.description.fil | Fil: Marziali, Federico Emanuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina. | |
| dc.description.fil | Fil: Bugnon Valdano, Marina Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina. | |
| dc.description.fil | Fil: Brunet Avalos, Clarise. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina. | |
| dc.description.fil | Fil: Moriena, Lucía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina. | |
| dc.description.fil | Fil: Cavatorta, Ana Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina. | |
| dc.description.fil | Fil: Gardiol, Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (CONICET); Argentina. | |
| dc.description.sponsorship | Fundación Bunge y Born for the Oster Fellowship to FM. | |
| dc.description.sponsorship | This work was partially supported by a grant from the Instituto Nacional del Cáncer, Ministerio de Salud de la República Argentina to DG. | |
| dc.format | application/pdf | |
| dc.format.extent | 355, 1-19 | |
| dc.identifier.issn | 1999-4915 | |
| dc.identifier.uri | http://hdl.handle.net/2133/10468 | |
| dc.language.iso | eng | es |
| dc.publisher | MDPI | es |
| dc.relation.publisherversion | http://dx.doi.or/doi:10.3390/v9120355 | es |
| dc.relation.publisherversion | http://www.mdpi.com/1999-4915/9/12/355 | es |
| dc.rights | openAccess | es |
| dc.rights.holder | Marziali, Federico Emanuel | es |
| dc.rights.holder | Bugnon Valdano, Marina Paula | es |
| dc.rights.holder | Brunet Avalos, Clarise | es |
| dc.rights.holder | Moriena, Lucía | es |
| dc.rights.holder | Cavatorta, Ana Laura | es |
| dc.rights.holder | Gardiol, Daniela | es |
| dc.rights.holder | Universidad Nacional de Rosario | es |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/2.5/ar/ | * |
| dc.subject | HTLV-1 | es |
| dc.subject | Tax | es |
| dc.subject | DLG1 | es |
| dc.subject | Protein Interactions | es |
| dc.subject | PDZ | es |
| dc.title | Interference of HTLV-1 Tax Protein with Cell Polarity Regulators: Defining the Subcellular Localization of the Tax-DLG1 Interaction | es |