Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity
dc.citation.title | Advances in Bioscience and Biotechnology | es |
dc.citation.volume | 2 | es |
dc.creator | Ruiz Álvarez, Jimena Inés | |
dc.creator | Teijeiro, Juan Manuel | |
dc.creator | Marini, Patricia Estela | |
dc.date.accessioned | 2017-12-04T16:42:51Z | |
dc.date.available | 2017-12-04T16:42:51Z | |
dc.date.issued | 2011-02 | |
dc.description | Immunological methods involving pregnancy associated glycoproteins (PAG) are used for cattle pregnancy detection. The faults of these methods could be overcomed by using antibodies specific for each member of the PAG family. In order to differentiate between very similar proteins, preparation of antibodies specific for peptides is a method of choice. In this work, we summarize a series of considerations regarding peptide design and choose free access NCBI, Antigenicity Plot, EMBOSS Antigenic and Expasy tools to apply them. We design peptides specific for different reported PAG members and obtain the corresponding polyclonal antibodies for five of them. | es |
dc.description.fil | Fil: Ruiz Álvarez, Jimena Inés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina | es |
dc.description.fil | Fil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina | es |
dc.description.fil | Fil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina Borrar seleccionados | es |
dc.description.sponsorship | Fundación Nuevo Banco de Santa Fe, Argentina | es |
dc.description.sponsorship | Secretaría de Estado de Ciencia, Tecnología e Innovación, Provincia de Santa Fe, Argentina | es |
dc.format | application/pdf | |
dc.format.extent | 84-87 | es |
dc.identifier.issn | 2156-8502 | es |
dc.identifier.uri | http://hdl.handle.net/2133/9731 | |
dc.language.iso | eng | es |
dc.publisher | SciRP | es |
dc.relation.publisherversion | http://file.scirp.org/Html/4613.html | es |
dc.relation.publisherversion | http://doi:10.4236/abb.2011.22013 | es |
dc.rights | openAccess | es |
dc.rights.holder | SCIRP | es |
dc.rights.holder | Ruiz Álvarez, Jimena Inés | es |
dc.rights.holder | Teijeiro, Juan Manuel | es |
dc.rights.holder | Marini, Patricia Estela | es |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | * |
dc.subject | Anticuerpo | es |
dc.subject | Péptido | es |
dc.subject | Bovino | es |
dc.subject | Preñez | es |
dc.subject | Antibodies | es |
dc.subject | Pregnancy | es |
dc.subject | Cattle | es |
dc.title | Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity | es |
dc.type | article | |
dc.type | artículo | |
dc.type | publishedVersion | |
dc.type.collection | articulo | |
dc.type.version | publishedVersion | es |