Preparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificity

dc.citation.titleAdvances in Bioscience and Biotechnologyes
dc.citation.volume2es
dc.creatorRuiz Álvarez, Jimena Inés
dc.creatorTeijeiro, Juan Manuel
dc.creatorMarini, Patricia Estela
dc.date.accessioned2017-12-04T16:42:51Z
dc.date.available2017-12-04T16:42:51Z
dc.date.issued2011-02
dc.descriptionImmunological methods involving pregnancy associated glycoproteins (PAG) are used for cattle pregnancy detection. The faults of these methods could be overcomed by using antibodies specific for each member of the PAG family. In order to differentiate between very similar proteins, preparation of antibodies specific for peptides is a method of choice. In this work, we summarize a series of considerations regarding peptide design and choose free access NCBI, Antigenicity Plot, EMBOSS Antigenic and Expasy tools to apply them. We design peptides specific for different reported PAG members and obtain the corresponding polyclonal antibodies for five of them.es
dc.description.filFil: Ruiz Álvarez, Jimena Inés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentinaes
dc.description.filFil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentinaes
dc.description.filFil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina Borrar seleccionadoses
dc.description.sponsorshipFundación Nuevo Banco de Santa Fe, Argentinaes
dc.description.sponsorshipSecretaría de Estado de Ciencia, Tecnología e Innovación, Provincia de Santa Fe, Argentinaes
dc.formatapplication/pdf
dc.format.extent84-87es
dc.identifier.issn2156-8502es
dc.identifier.urihttp://hdl.handle.net/2133/9731
dc.language.isoenges
dc.publisherSciRPes
dc.relation.publisherversionhttp://file.scirp.org/Html/4613.htmles
dc.relation.publisherversionhttp://doi:10.4236/abb.2011.22013es
dc.rightsopenAccesses
dc.rights.holderSCIRPes
dc.rights.holderRuiz Álvarez, Jimena Inéses
dc.rights.holderTeijeiro, Juan Manueles
dc.rights.holderMarini, Patricia Estelaes
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/2.5/ar/*
dc.subjectAnticuerpoes
dc.subjectPéptidoes
dc.subjectBovinoes
dc.subjectPreñezes
dc.subjectAntibodieses
dc.subjectPregnancyes
dc.subjectCattlees
dc.titlePreparation of monospecific anti-PAG antibodies for cattle pregnancy detection: use of synthetic peptides to improve specificityes
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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