Examinando por Autor "Bustamante, Claudia Anabel"
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Ítem Embargo Caracterización in silico y análisis funcional de nuevos factores implicados en la protección frente al daño por frío en frutos(2024) Aimaro, Nicolás; Bustamante, Claudia Anabel; Gismondi, MauroLa refrigeración es una de las herramientas más utilizadas para prolongar la vida poscosecha de los tomates. Las bajas temperaturas permiten retrasar el proceso de maduración y mantener la calidad del fruto durante el almacenamiento, transporte y comercialización. Sin embargo, bajo estas condiciones el tomate se ve afectado por desórdenes fisiológicos denominados genéricamente “daño por frío”, los que se evidencian en el momento del consumo y que difícilmente pueden ser percibidos en las etapas previas de la cadena de comercialización. En la presente tesina se elaboró un catálogo de genes de Solanum lycopersicum, utilizando Arabidopsis thaliana como modelo para identificar genes ortólogos relacionados con la respuesta al frío. Adicionalmente, se llevaron a cabo análisis de expresión de los genes HD2C (histona desacetilasa 2c) obteniéndose una correlación entre los estudios in silico y la respuesta al estimulo por bajas temperaturas en la especie de interés. Los resultados obtenidos sugieren que una amplia diversidad de procesos celulares forman parte de la respuesta de los frutos frente al frío. Evaluar si HD2C brinda una protección frente al daño por frio será esencial para lograr obtener variedades con mayor calidad poscosecha.Ítem Acceso Abierto Deciphering the metabolic pathways influencing heat and cold responses during post-harvest physiology of peach fruit(Wiley, 2014-01-21) Lauxmann, Martín Alexander; Borsani, Julia; Osorio, Sonia; Lombardo, Verónica Andrea; Budde, Claudio O.; Bustamante, Claudia Anabel; Monti, Laura Lucía; Andreo, Carlos Santiago; Fernie, Alisdair R.; Drincovich, María Fabiana; Lara, María ValeriaPeaches are highly perishable and deteriorate quickly at ambient temperature. Cold storage is commonly used to prevent fruit decay; however, it affects fruit quality causing physiological disorders collectively termed ‘chilling injury’ (CI). To prevent or ameliorate CI, heat treatment is often applied prior to cold storage. In the present work, metabolic profiling was performed to determine the metabolic dynamics associated with the induction of acquired CI tolerance in response to heat shock. ‘Dixiland’ peach fruits exposed to 39 °C, cold stored, or after a combined treatment of heat and cold, were compared with fruits ripening at 20 °C. Dramatic changes in the levels of compatible solutes such as galactinol and raffinose were observed, while amino acid precursors of the phenylpropanoid pathway were also modified due to the stress treatments, as was the polyamine putrescine. The observed responses towards temperature stress in peaches are composed of both common and specific response mechanisms to heat and cold, but also of more general adaptive responses that confer strategic advantages in adverse conditions such as biotic stresses. The identification of such key metabolites, which prime the fruit to cope with different stress situations, will likely greatly accelerate the design and the improvement of plant breeding programs.Ítem Acceso Abierto Differential metabolic rearrangements after cold storage are correlated with chilling injury resistance of peach fruits(Frontiers Media, 2016-09-30) Bustamante, Claudia Anabel; Monti, Laura Lucía; Gabilondo, Julieta; Scossa, Federico; Budde, Claudio O.; Lara, María Valeria; Fernie, Alisdair R.; Drincovich, María FabianaÍtem Embargo Estudio de un factor implicado en la protección frente al daño por frío en duraznos en la especie modelo Arabidopsis thaliana(2017) Strologo, Laura; Bustamante, Claudia Anabel; Gismondi, MauroUna vez cosechados, los duraznos son almacenados a bajas temperaturas para retrasar su maduración, lo cual puede producir síntomas de daño por frío afectando su calidad. Entre otras estrategias, el tratamiento con calor precediendo al almacenamiento en frío ha resultado ser efectivo previniendo los síntomas indeseados. PpTRAF se identificó como transcripto inducido por tratamiento térmico y bajas temperaturas. El mismo codifica para una proteína con dedos de zinc de tipo TRAF, verificada experimentalmente como ARN chaperona. En la presente tesina se caracterizó al gen AtTRAF, ortólogo de PpTRAF en Arabidopsis thaliana, cuya funcionalidad es desconocida. La abundancia del ARNm AtTRAF se analizó en distintos órganos de la especie en estudio, alcanzando niveles superiores en órganos florales. Adicionalmente, se observó un aumento en los niveles del transcripto a altas temperaturas, así como también luego de tratamientos por frío en plantas adultas. Por otro lado, se obtuvieron genotipos mutantes KO y sobreexpresantes de AtTRAF sobre los cuales se analizaron rasgos fenotípicos tales como la capacidad de germinar y elongar raíces bajo estímulos de frío y calor, así como también en presencia de ABA, NaCl, H2O2 o manitol. Variadas respuestas frente a tratamientos con temperatura fueron observadas para los genotipos relacionados a AtTRAF respecto a la línea salvaje col-0. Finalmente, utilizando un ensayo de complementación in vivo en la cepa de E. coli RL211, la actividad ARN chaperona de AtTRAF fue confirmada. En conjunto, los resultados obtenidos en el presente trabajo sugieren que AtTRAF podría cumplir un rol molecular como ARN chaperona en respuestas de A. thaliana frente a bajas y altas temperaturas de manera similar a la que podría llevar a cabo PpTRAF en frutos de durazno.Ítem Acceso Abierto Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease(Wiley, 2025-06-02) Novello, María Angelina; Bustamante, Claudia Anabel; Svetaz, Laura Andrea; Goldy, Camila; Valentini, Gabriel Hugo; Drincovich, María Fabiana; Brotman, Yariv; Fernie, Alisdair R.; Lara, María Valeria; https://orcid.org/0000-0003-4914-0242Peach Leaf Curl Disease, caused by Taphrina deformans, is characterized by reddish hypertrophic and hyperplasic leaf areas. To comprehend the biochemical imbalances caused by the fungus, dissected symptomatic (C) and asymptomatic areas (N) from leaves with increasing disease extension were analyzed by an integrated approach including metabolomics, lipidomics, proteomics, and complementary biochemical techniques. Drastic metabolic differences were identified in C areas with respect to either N areas or healthy leaves, including altered chloroplastic functioning and composition, which differs from the typical senescence process. In C areas, alteration in redox-homoeostasis proteins and in triacylglycerols content, peroxidation and double bond index were observed. Proteomic data revealed induction of host enzymes involved in auxin and jasmonate biosynthesis and an upregulation of phenylpropanoid and mevalonate pathways and downregulation of the plastidic methylerythritol phosphate route. Amino acid pools were affected, with upregulation of proteins involved in asparagine synthesis. Curled areas exhibited a metabolic shift towards functioning as a sink tissue importing sugars, probably from N areas, and producing energy through fermentation and respiration and reductive power via the pentose phosphate route. Identifying the metabolic disturbances leading to disease symptoms is a key step in designing strategies to prevent or delay the progression of the disease.Ítem Acceso Abierto Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissues(Frontiers Media, 2019-06-04) Rodriguez, Carlos E.; Bustamante, Claudia Anabel; Budde, Claudio O.; Müller, Gabriela Leticia; Drincovich, María Fabiana; Lara, María ValeriaÍtem Acceso Abierto Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes(Wiley, 2017-07-12) Svetaz, Laura Andrea; Bustamante, Claudia Anabel; Goldy, Camila; Rivero, Nery Alberto; Müller, Gabriela Leticia; Valentini, Gabriel Hugo; Fernie, Alisdair R.; Drincovich, María Fabiana; Lara, María Valeria; https://orcid.org/0000-0003-4914-0242; Dr. Bellini, E.: provide P. persica selections DOFI-84.364.089 and DOFI-84.364.060; Dr. Giordani, E.: provide P. persica selections DOFI-84.364.089 and DOFI-84.364.060Leaf peach curl is a devastating disease affecting leaves, flowers and fruits, caused by the dimorphic fungus Taphrina deformans. To gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible Prunus persica genotypes were inoculated with blastospores (yeast), and the infection was monitored during 120 h post inoculation (h.p.i.). Fungal dimorphism to the filamentous form and induction of reactive oxygen species (ROS), callose synthesis, cell death and defence compound production were observed independently of the genotype. Fungal load significantly decreased after 120 h.p.i. in the resistant genotype, while the pathogen tended to grow in the susceptible genotypes. Metabolic profiling revealed a biphasic re-programming of plant tissue in susceptible genotypes, with an initial stage co-incident with the yeast form of the fungus and a second when the hypha is developed. Transcriptional analysis of PRs and plant hormone-related genes indicated that pathogenesis-related (PR) proteins are involved in P. persica defence responses against T. deformans and that salicylic acid is induced in the resistant genotype. Conducted experiments allowed the elucidation of common and differential responses in susceptible versus resistant genotypes and thus allow us to construct a picture of early events during T. deformans infection.