Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes

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dc.citation.titleMolecular Plant Pathology
dc.citation.volume18
dc.creatorRoeschlin, Roxana Andrea
dc.creatorFavaro, María Alejandra
dc.creatorChiesa, María Amalia
dc.creatorAlemano, Sergio Gabriel
dc.creatorVojnov, Adrián Alberto
dc.creatorCastagnaro, Atilio Pedro
dc.creatorFilippone, María Paula
dc.creatorGmitter, Frederick George
dc.creatorGadea, José
dc.creatorMarano, María Rosa
dc.date.accessioned2024-05-20T20:05:40Z
dc.date.available2024-05-20T20:05:40Z
dc.date.issued2017-11-08
dc.description.abstractXanthomonas citri ssp. citri (X. citri) is the causal agent of Asiatic citrus canker, a disease that seriously affects most commercially important Citrus species worldwide. We have identified previously a natural variant, X. citri AT, that triggers a host-specific defence response in Citrus limon. However, the mechanisms involved in this canker disease resistance are unknown. In this work, the defence response induced by X. citri AT was assessed by transcriptomic, physiological and ultrastructural analyses, and the effects on bacterial biofilm formation were monitored in parallel. We show that X. citri AT triggers a hypersensitive response associated with the interference of biofilm development and arrest of bacterial growth in C. limon. This plant response involves an extensive transcriptional reprogramming, setting in motion cell wall reinforcement, the oxidative burst and the accumulation of salicylic acid (SA) and phenolic compounds. Ultrastructural analyses revealed subcellular changes involving the activation of autophagy-associated vacuolar processes. Our findings show the activation of SA-dependent defence in response to X. citri AT and suggest a coordinated regulation between the SA and flavonoid pathways, which is associated with autophagy mechanisms that control pathogen invasion in C. limon. Furthermore, this defence response protects C. limon plants from disease on subsequent challenges by pathogenic X. citri. This knowledge will allow the rational exploitation of the plant immune system as a biotechnological approach for the management of the disease.
dc.description.filFil: Roeschlin, Roxana Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Área Virología; Argentina.
dc.description.filFil: Favaro, María Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Área Virología; Argentina.
dc.description.filFil: Chiesa, María Amalia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Área Virología; Argentina.
dc.description.filFil: Marano, María Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Área Virología; Argentina.
dc.description.filFil: Alemano, Sergio Gabriel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal; Argentina.
dc.description.filFil: Vojnov, Adrián Alberto. Fundación Pablo Cassará (FPC-CONICET). Instituto de Ciencia y Tecnología César Milstein (ICT Milstein); Argentina.
dc.description.filFil: Castagnaro, Atilio Pedro. Estación Experimental Agroindustrial Obispo Colombres (EEAOC-CONICET). Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITA-NOA); Argentina.
dc.description.filFil: Filippone, María Paula. Estación Experimental Agroindustrial Obispo Colombres (EEAOC-CONICET). Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITA-NOA); Argentina.
dc.description.filFil: Gmitter, Frederick George. University of Florida. Citrus Research and Education Center (CREC); United States.
dc.description.filFil: Gadea, José. Universitat Politècnica de València. Ciudad Politécnica de la Innovación (CPI). Instituto de Biología Molecular y Celular de Plantas (IBMCP-CSIC); España.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT-2011-1833
dc.description.sponsorshipFlorida Citrus Research and Development Foundation
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.versionpeerreviewed
dc.format.extent1-15
dc.identifier.e-issn1364-3703
dc.identifier.issn1464-6722
dc.identifier.urihttps://hdl.handle.net/2133/27072
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1111/mpp.12489
dc.relation.publisherversionhttps://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12489
dc.rightsopenAccess
dc.rights.holderRoeschlin, Roxana Andrea
dc.rights.holderFavaro, María Alejandra
dc.rights.holderChiesa, María Amalia
dc.rights.holderAlemano, Sergio Gabriel
dc.rights.holderVojnov, Adrián Alberto
dc.rights.holderCastagnaro, Atilio Pedro
dc.rights.holderFilippone, María Paula
dc.rights.holderGmitter, Frederick George
dc.rights.holderGadea, José
dc.rights.holderMarano, María Rosa
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectAutophagy
dc.subjectBiofilm formation
dc.subjectBiological control
dc.subjectCitrus canker resistance
dc.titleResistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes
dc.typearticulo
dc.type.versionpublishedVersion

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