NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems

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dc.citation.titleNature Communicationses
dc.citation.volume14
dc.creatorMolinari, Pamela Estefanía
dc.creatorKrapp, Adriana R.
dc.creatorWeiner, Andrea María Julia
dc.creatorBeyer, Hannes M.
dc.creatorKondadi, Arun Kumar
dc.creatorBlomeier, Tim
dc.creatorLópez, Melina
dc.creatorBustos Sanmamed, Pilar
dc.creatorTevere, Evelyn
dc.creatorWeber, Wilfried
dc.creatorReichert, Andreas S.
dc.creatorCalcaterra, Nora B.
dc.creatorBeller, Mathias
dc.creatorCarrillo, Néstor
dc.creatorZurbriggen, Matias D.
dc.date.accessioned2023-08-15T15:33:31Z
dc.date.available2023-08-15T15:33:31Z
dc.date.issued2023-06-06
dc.descriptionNADP(H) is a central metabolic hub providing reducing equivalents to multiple biosynthetic, regulatory and antioxidative pathways in all living organisms. While biosensors are available to determine NADP+ or NADPH levels in vivo, no probe exists to estimate the NADP(H) redox status, a determinant of the cell energy availability. We describe herein the design and characterization of a genetically-encoded ratiometric biosensor, termed NERNST, able to interact with NADP(H) and estimate ENADP(H). NERNST consists of a redox-sensitive green fluorescent protein (roGFP2) fused to an NADPH-thioredoxin reductase C module which selectively monitors NADP(H) redox states via oxidoreduction of the roGFP2 moiety. NERNST is functional in bacterial, plant and animal cells, and organelles such as chloroplasts and mitochondria. Using NERNST, we monitor NADP(H) dynamics during bacterial growth, environmental stresses in plants, metabolic challenges to mammalian cells, and wounding in zebrafish. NERNST estimates the NADP(H) redox poise in living organisms, with various potential applications in biochemical, biotechnological and biomedical research.es
dc.description.filFil: Molinari, Pamela Estefanía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Krapp, Adriana R. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Weiner, Andrea María Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Beyer, Hannes M. University of Düsseldorf. Institute of Synthetic Biology; Germany.es
dc.description.filFil: Kondadi, Arun Kumar. Heinrich-Heine-University Düsseldor. Medical Faculty and University Hospital Düsseldorf. Institute of Biochemistry and Molecular Biology I; Germany.es
dc.description.filFil: Blomeier, Tim.University of Düsseldorf. Institute of Synthetic Biology; Germany.es
dc.description.filFil: López, Melina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Bustos Sanmamed, Pilar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Tevere, Evelyn. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Weber, Wilfried. University of Freiburg. Faculty of Biology and Signalling Research Centres BIOSS and CIBSS; Germany.es
dc.description.filFil: Weber, Wilfried. Saarland University. Leibniz Institute for New Materials and Department of Materials Sciences and Engineering; Germany.es
dc.description.filFil: Reichert, Andreas S. Heinrich-Heine-University Düsseldor. Medical Faculty and University Hospital Düsseldorf. Institute of Biochemistry and Molecular Biology I; Germany.es
dc.description.filFil: Calcaterra, Nora B. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Beller, Mathias. University of Düsseldorf. Institute of Mathematical Modeling of Biological Systems; Germany.es
dc.description.filFil: Carrillo, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Zurbriggen, Matias D. University of Düsseldorf. Institute of Synthetic Biology; Germany.es
dc.description.filFil: Zurbriggen, Matias D. Cluster of Excellence on Plant Sciences; Germany.es
dc.description.sponsorshipNational Agency for the Promotion of Science and Technologyes
dc.description.sponsorshipDeutscher Akademischer Austauschdienst (DAAD)es
dc.description.sponsorshipDeutsche Forschungsgemeinschaft (DFG): 194465578, 267205415, 390686111, 390939984, 391465903/GRK 2466, 458090666, CEPLAS-EXC-1028, CIBSS-EXC-2189, EXC-2048, HFSP RGP 0067, INST 208/760-1FUGG, IRTG1525, PICT-2015.3828, PICT-2019-03722, SFB1535es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT)es
dc.formatapplication/pdf
dc.format.extent1-18es
dc.identifier.issn2041-1723es
dc.identifier.urihttp://hdl.handle.net/2133/26197
dc.language.isoenges
dc.publisherNature Researches
dc.relation.publisherversionhttps://doi.org/10.1038/s41467-023-38739-4
dc.relation.publisherversionhttps://www.nature.com/articles/s41467-023-38739-4
dc.rightsopenAccesses
dc.rights.holderMolinari, Pamela Estefaníaes
dc.rights.holderKrapp, Adriana R.es
dc.rights.holderWeiner, Andrea María Juliaes
dc.rights.holderBeyer, Hannes M.es
dc.rights.holderKondadi, Arun Kumares
dc.rights.holderBlomeier, Times
dc.rights.holderLópez, Melinaes
dc.rights.holderBustos Sanmamed, Pilares
dc.rights.holderTevere, Evelynes
dc.rights.holderWeber, Wilfriedes
dc.rights.holderReichert, Andreas S.es
dc.rights.holderCalcaterra, Nora B.es
dc.rights.holderBeller, Mathiases
dc.rights.holderCarrillo, Néstores
dc.rights.holderZurbriggen, Matias D.es
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectAnimalses
dc.subjectChloroplastses
dc.subjectMammalses
dc.subjectNADPes
dc.subjectOxidation-reductiones
dc.subjectPlantses
dc.subjectZebrafishes
dc.titleNERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systemses
dc.typepublishedVersion
dc.typeartículo
dc.typearticle
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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