The stringent response plays a key role in Bacillus subtilis survival of fatty acid starvation

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dc.citation.titleMolecular Microbiology
dc.citation.volume103
dc.creatorPulschen, André A.
dc.creatorSastre, Diego Emiliano
dc.creatorMachinandiarena, Federico
dc.creatorCrotta Asis, Agostina
dc.creatorAlbanesi, Daniela
dc.creatorDe Mendoza, Diego
dc.creatorGueiros-Filho, Frederico J.
dc.date.accessioned2024-12-18T15:14:08Z
dc.date.available2024-12-18T15:14:08Z
dc.date.issued2017-02-07
dc.description.abstractThe stringent response is a universal adaptive mechanism to protect bacteria from nutritional and environmental stresses. The role of the stringent response during lipid starvation has been studied only in Gram-negative bacteria. Here, we report that the stringent response also plays a crucial role in the adaptation of the model Gram-positive Bacillus subtilis to fatty acid starvation. B. subtilis lacking all three (p)ppGpp-synthetases (RelBs, RelP and RelQ) or bearing a RelBs variant that no longer synthesizes (p)ppGpp suffer extreme loss of viability on lipid starvation. Loss of viability is paralleled by perturbation of membrane integrity and function, with collapse of membrane potential as the likely cause of death. Although no increment of (p)ppGpp could be detected in lipid starved B. subtilis, we observed a substantial increase in the GTP/ATP ratio of strains incapable of synthesizing (p)ppGpp. Artificially lowering GTP with decoyinine rescued viability of such strains, confirming observations that low intracellular GTP is important for survival of nutritional stresses. Altogether, our results show that activation of the stringent response by lipid starvation is a broadly conserved response of bacteria and that a key role of (p)ppGpp is to couple biosynthetic processes that become detrimental if uncoordinated.
dc.description.filFil: Pulschen, André A. Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica; Brasil.
dc.description.filFil: Sastre, Diego Emiliano. Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica; Brasil.
dc.description.filFil: Machinandiarena, Federico. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Crotta Asis, Agostina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Albanesi, Daniela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Gueiros-Filho, Frederico J. Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica; Brasil.
dc.description.sponsorshipCAPES. Programa Pesquisador Visitante Especial: 1169/2013
dc.description.sponsorshipFAPESP: 2014/26528-4, 2014/13411-1
dc.description.versionpeerreviewed
dc.format.extent1-15
dc.identifier.e-issn1365-2958
dc.identifier.issn0950-382X
dc.identifier.urihttps://hdl.handle.net/2133/28489
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1111/mmi.13582
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/mmi.13582
dc.rightsopenAccess
dc.rights.holderPulschen, André A.
dc.rights.holderSastre, Diego Emiliano
dc.rights.holderMachinandiarena, Federico
dc.rights.holderCrotta Asis, Agostina
dc.rights.holderAlbanesi, Daniela
dc.rights.holderDe Mendoza, Diego
dc.rights.holderGueiros-Filho, Frederico J.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBacillus subtilis
dc.subjectStringent response
dc.subjectFatty Acids starvation
dc.subjectGuanosine Tetraphosphate
dc.subjectppGpp
dc.subjectGuanosine Triphosphate
dc.subjectGTP
dc.titleThe stringent response plays a key role in Bacillus subtilis survival of fatty acid starvation
dc.typearticulo
dc.type.versionpublishedVersion

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