Gene expression changes throughout the life cycle allow a bacterial plant pathogen to persist in diverse environmental habitats

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dc.citation.titlePLoS Pathogens
dc.citation.volume19
dc.contributor.orcidhttps://orcid.org/0000-0003-2312-0091
dc.creatorDe Pedro-Jové, Roger
dc.creatorCorral, Jordi
dc.creatorRocafort, Mercedes
dc.creatorPuigvert, Marina
dc.creatorAzam, Fàtima Latif
dc.creatorVandecaveye, Agustina
dc.creatorMacho, Alberto P.
dc.creatorBalsalobre , Carlos
dc.creatorColl, Núria S.
dc.creatorOrellano, Elena G.
dc.creatorValls, Marc
dc.date.accessioned2024-06-10T12:41:46Z
dc.date.available2024-06-10T12:41:46Z
dc.date.issued2023-12-19
dc.description.abstractBacterial pathogens exhibit a remarkable ability to persist and thrive in diverse ecological niches. Understanding the mechanisms enabling their transition between habitats is crucial to control dissemination and potential disease outbreaks. Here, we use Ralstonia solanacearum, the causing agent of the bacterial wilt disease, as a model to investigate pathogen adaptation to water and soil, two environments that act as bacterial reservoirs, and compare this information with gene expression in planta. Gene expression in water resembled that observed during late xylem colonization, with an intriguing induction of the type 3 secretion system (T3SS). Alkaline pH and nutrient scarcity—conditions also encountered during late infection stages–were identified as the triggers for this T3SS induction. In the soil environment, R. solanacearum upregulated stress-responses and genes for the use of alternate carbon sources, such as phenylacetate catabolism and the glyoxylate cycle, and downregulated virulence-associated genes. We proved through gain- and loss-of-function experiments that genes associated with the oxidative stress response, such as the regulator OxyR and the catalase KatG, are key for bacterial survival in soil, as their deletion cause a decrease in culturability associated with a premature induction of the viable but non culturable state (VBNC). This work identifies essential factors necessary for R. solanacearum to complete its life cycle and is the first comprehensive gene expression analysis in all environments occupied by a bacterial plant pathogen, providing valuable insights into its biology and adaptation to unexplored habitats.
dc.description.filFil: De Pedro-Jové, Roger. University of Barcelona. Department of Genetics, Microbiology and Statistics; Spain.
dc.description.filFil: De Pedro-Jové, Roger. Centre for Research in Agricultural Genomics; Spain.
dc.description.filFil: Corral, Jordi. Centre for Research in Agricultural Genomics; Spain.
dc.description.filFil: Rocafort, Mercedes. Centre for Research in Agricultural Genomics; Spain.
dc.description.filFil: Puigvert, Marina. University of Barcelona. Department of Genetics, Microbiology and Statistics; Spain.
dc.description.filFil: Puigvert, Marina. Centre for Research in Agricultural Genomics; Spain.
dc.description.filFil: Azam, Fàtima Latif. University of Barcelona. Department of Genetics, Microbiology and Statistics; Spain.
dc.description.filFil: Azam, Fàtima Latif. Centre for Research in Agricultural Genomics; Spain.
dc.description.filFil: Vandecaveye, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular. Instituto de Biología Molecular y Celular de Rosario (IBR- CONICET); Argentina.
dc.description.filFil: Macho, Alberto P. Chinese Academy of Sciences. Centre for Excellence in Molecular Plant Sciences. Shanghai Centre for Plant Stress Biology; China.
dc.description.filFil: Balsalobre, Carlos. University of Barcelona. Department of Genetics, Microbiology and Statistics; Spain.
dc.description.filFil: Coll, Núria S. Centre for Research in Agricultural Genomics; Spain.
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular. Instituto de Biología Molecular y Celular de Rosario (IBR- CONICET); Argentina.
dc.description.filFil: Valls, Marc. University of Barcelona. Department of Genetics, Microbiology and Statistics; Spain.
dc.description.filFil: Valls, Marc. Centre for Research in Agricultural Genomics; Spain.
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MCIN): AEI/PID2019-108595RB-I00
dc.description.sponsorshipFundación Carmen y Severo Ochoa: CEX2019-000917
dc.description.sponsorshipMinisterio de Universidades: FPU2018-03285
dc.description.sponsorshipSecretaria d’Universitats i Recerca del Departament d’Empresa i Coneixement de la Generalitat de Catalunya: FI_B 00461
dc.description.versionpeerreviewed
dc.format.extent1-26
dc.identifier.issn1553-7374
dc.identifier.urihttps://hdl.handle.net/2133/27280
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.publisherversionhttps://doi.org/10.1371/journal.ppat.1011888
dc.relation.publisherversionhttps://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1011888
dc.rightsopenAccess
dc.rights.holderDe Pedro-Jové, Roger
dc.rights.holderCorral, Jordi
dc.rights.holderRocafort, Mercedes
dc.rights.holderPuigvert, Marina
dc.rights.holderAzam, Fàtima Latif
dc.rights.holderVandecaveye, Agustina
dc.rights.holderMacho, Alberto P.
dc.rights.holderBalsalobre, Carlos
dc.rights.holderColl, Núria S.
dc.rights.holderOrellano, Elena G.
dc.rights.holderValls, Marc
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectGene expression
dc.subjectLife cycle stages
dc.subjectPlant diseases
dc.subjectRalstonia solanacearum
dc.subjectSolanum lycopersicum
dc.titleGene expression changes throughout the life cycle allow a bacterial plant pathogen to persist in diverse environmental habitats
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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