Core clock genes adjust growth cessation time to day-night switches in poplar
dc.citation.title | Nature Communications | |
dc.citation.volume | 1-9 | |
dc.contributor.orcid | https://orcid.org/0000-0001-7869-9411 | |
dc.contributor.orcid | https://orcid.org/0000-0002-7012-2850 | |
dc.contributor.orcid | https://orcid.org/0000-0001-7263-0560 | |
dc.contributor.orcid | https://orcid.org/0000-0002-7351-8439 | |
dc.creator | Alique García, Daniel | |
dc.creator | Redondo López, Arturo | |
dc.creator | González Schain, Nahuel | |
dc.creator | Allona, Isabel | |
dc.creator | Wabnik, Krzysztof | |
dc.creator | Perales, Mariano | |
dc.date.accessioned | 2024-03-19T12:45:30Z | |
dc.date.available | 2024-03-19T12:45:30Z | |
dc.date.issued | 2024-02-27 | |
dc.description.abstract | Poplar trees use photoperiod as a precise seasonal indicator, synchronizing plant phenology with the environment. Daylength cue determines FLOWERING LOCUS T 2 (FT2) daily expression, crucial for shoot apex development and establishment of the annual growing period. However, limited evidence exists for the molecular factors controlling FT2 transcription and the conservation with the photoperiodic control of Arabidopsis flowering. We demonstrate that FT2 expression mediates growth cessation response quantitatively, and we provide a minimal data-driven model linking core clock genes to FT2 daily levels. GIGANTEA (GI) emerges as a critical inducer of the FT2 activation window, time-bound by TIMING OF CAB EXPRESSION (TOC1) and LATE ELONGATED HYPOCOTYL (LHY2) repressions. CRISPR/Cas9 loss-of-function lines validate these roles, identifying TOC1 as a long-sought FT2 repressor. Additionally, model simulations predict that FT2 downregulation upon daylength shortening results from a progressive narrowing of this activation window, driven by the phase shift observed in the preceding clock genes. This circadian-mediated mechanism enables poplar to exploit FT2 levels as an accurate daylength-meter. | |
dc.description.fil | Fil: Alique García, Daniel. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC); Spain. | |
dc.description.fil | Fil: Redondo López, Arturo. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC); Spain. | |
dc.description.fil | Fil: González Schain, Nahuel. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC); Spain. | |
dc.description.fil | Fil: Allona, Isabel. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC); Spain. | |
dc.description.fil | Fil: Wabnik, Krzysztof. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC); Spain. | |
dc.description.fil | Fil: Perales, Mariano. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC); Spain. | |
dc.description.fil | Fil: Allona, Isabel. Universidad Politécnica de Madrid. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas. Departamento de Biotecnología-Biología Vegetal; Spain. | |
dc.description.fil | Fil: Wabnik, Krzysztof. Universidad Politécnica de Madrid. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas. Departamento de Biotecnología-Biología Vegetal; Spain. | |
dc.description.fil | Fil: Perales, Mariano. Universidad Politécnica de Madrid. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas. Departamento de Biotecnología-Biología Vegetal; Spain. | |
dc.description.fil | Fil: González Schain, Nahuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.sponsorship | Ministerio de Ciencia Innovacion y Universidades of Spain: PGC2018-093922-B-100, PID2021-123060OB-I00, PID2021-122158NB-I00 | |
dc.description.sponsorship | Programa de Atraccion de Talento 2017 Comunidad de Madrid: 2021-5 A/BIO-20952 | |
dc.description.sponsorship | Severo Ochoa Program for Centres of Excellence in R&D Agencia Estatal de Investigación of Spain: CEX2020-000999-S | |
dc.description.sponsorship | Ministerio de Universidades of Spain: FPU19/04729 | |
dc.description.sponsorship | Maria Zambrano grant: UP2021-035 | |
dc.description.version | peerreviewed | |
dc.identifier.e-issn | 2041-1723 | |
dc.identifier.uri | https://hdl.handle.net/2133/26807 | |
dc.language.iso | en | |
dc.publisher | Springer Nature | |
dc.relation.publisherversion | https://doi.org/10.1038/s41467-024-46081-6 | |
dc.relation.publisherversion | https://www.nature.com/articles/s41467-024-46081-6 | |
dc.rights | openAccess | |
dc.rights.holder | Alique García, Daniel | |
dc.rights.holder | Redondo López, Arturo | |
dc.rights.holder | González Schain, Nahuel | |
dc.rights.holder | Allona, Isabel | |
dc.rights.holder | Wabnik, Krzysztof | |
dc.rights.holder | Perales, Mariano | |
dc.rights.holder | Universidad Nacional de Rosario | |
dc.rights.text | Attribution 4.0 International | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Arabidopsis Proteins genetics | |
dc.subject | Arabidopsis Proteins metabolism | |
dc.subject | Arabidopsis metabolism | |
dc.subject | Circadian Rhythm genetics | |
dc.subject | Flowers metabolism | |
dc.subject | Gene Expression Regulation, Plant | |
dc.subject | Hypocotyl metabolism | |
dc.subject | Photoperiod | |
dc.subject | Populus metabolism | |
dc.title | Core clock genes adjust growth cessation time to day-night switches in poplar | |
dc.type | articulo | |
dc.type.collection | articulo | |
dc.type.version | publishedVersion |