Unsaturated long chain free fatty acids are input signals of the Salmonella enterica PhoP/PhoQ regulatory system

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dc.citation.titleJournal of Biological Chemistry (JBC)
dc.citation.volume288
dc.creatorViarengo, Gastón
dc.creatorSciara, Mariela Inés
dc.creatorSalazar, Mario Oscar
dc.creatorKieffer, Pablo M.
dc.creatorFurlán, Ricardo Luis Eugenio
dc.creatorGarcía Véscovi, Eleonora
dc.date.accessioned2024-05-17T18:55:38Z
dc.date.available2024-05-17T18:55:38Z
dc.date.issued2013-08-02
dc.description.abstractThe Salmonella enterica serovar Typhimurium PhoP/PhoQ system has largely been studied as a paradigmatic two-component regulatory system not only to dissect structural and functional aspects of signal transduction in bacteria but also to gain knowledge about the versatile devices that have evolved allowing a pathogenic bacterium to adjust to or counteract environmental stressful conditions along its life cycle. Mg2+ limitation, acidic pH, and the presence of cationic antimicrobial peptides have been identified as cues that the sensor protein PhoQ can monitor to reprogram Salmonella gene expression to cope with extra- or intracellular challenging conditions. In this work, we show for the first time that long chain unsaturated free fatty acids (LCUFAs) present in Salmonella growth medium are signals specifically detected by PhoQ. We demonstrate that LCUFAs inhibit PhoQ autokinase activity, turning off the expression of the PhoP-dependent regulon. We also show that LCUFAs exert their action independently of their cellular uptake and metabolic utilization by means of the β-oxidative pathway. Our findings put forth the complexity of input signals that can converge to finely tune the activity of the PhoP/PhoQ system. In addition, they provide a new potential biochemical platform for the development of antibacterial strategies to fight against Salmonella infections.
dc.description.filFil: Viarengo, Gastón. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Sciara, Mariela Inés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Salazar, Mario Oscar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; Argentina.
dc.description.filFil: Kieffer, Pablo M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Furlán, Ricardo Luis Eugenio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacognosia; Argentina.
dc.description.filFil: García Véscovi, Eleonora. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i)
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipSecretaría de Estado de Ciencia, Tecnología e Innovación (SECTeI)
dc.description.versionpeerreviewed
dc.format.extent1-13
dc.identifier.e-issn1083-351X
dc.identifier.issn0021-9258
dc.identifier.urihttps://hdl.handle.net/2133/27065
dc.language.isoen
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.relation.publisherversionhttps://doi.org/10.1074/jbc.M113.472829
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0021925820454006?via%3Dihub
dc.rightsopenAccess
dc.rights.holderViarengo, Gastón
dc.rights.holderSciara, Mariela Inés
dc.rights.holderSalazar, Mario Oscar
dc.rights.holderKieffer, Pablo M.
dc.rights.holderFurlán, Ricardo Luis Eugenio
dc.rights.holderGarcía Véscovi, Eleonora
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBacterial pathogenesis
dc.subjectBacterial protein kinases
dc.subjectBacterial signal transduction
dc.subjectBacterial transcription
dc.subjectHistidine kinases
dc.subjectPhoP/PhoQ input signal
dc.subjectSalmonella
dc.subjectTwo-component system
dc.subjectUnsaturated fatty acids
dc.titleUnsaturated long chain free fatty acids are input signals of the Salmonella enterica PhoP/PhoQ regulatory system
dc.typearticulo
dc.type.versionpublishedVersion

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