KatG, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaves

Vista sencilla de ítem
dc.citation.titlePLOS ONEes
dc.citation.volume11(3)es
dc.creatorTondo, María Laura
dc.creatorDelprato, María Laura
dc.creatorKraiselburd, Ivana
dc.creatorFernández Zenoff, María Verónica
dc.creatorFarías, María Eugenia
dc.creatorOrellano, Elena G.
dc.date.accessioned2021-02-23T20:10:29Z
dc.date.available2021-02-23T20:10:29Z
dc.date.issued2016-03-18
dc.descriptionXanthomonas citri subsp. citri (Xcc) is the bacterium responsible for citrus canker. This bacterium is exposed to reactive oxygen species (ROS) at different points during its life cycle, including those normally produced by aerobic respiration or upon exposition to ultraviolet (UV) radiation. Moreover, ROS are key components of the host immune response. Among enzymatic ROS-detoxifying mechanisms, catalases eliminate H2O2, avoiding the potential damage caused by this specie. Xcc genome includes four catalase genes. In this work, we studied the physiological role of KatG, the only bifunctional catalase of Xcc, through the construction and characterization of a modified strain (XcckatG), carrying an insertional mutation in the katG gene. First, we evaluated the involvement of KatG in the bacterial adaptive response to H2O2. XcckatG cultures exhibited lower catalase activity than those of the wild-type strain, and this activity was not induced upon treatment with sub-lethal doses of H2O2. Moreover, the KatG-deficient mutant exhibited decreased tolerance to H2O2 toxicity compared to wild-type cells and accumulated high intracellular levels of peroxides upon exposure to sub-lethal concentrations of H2O2. To further study the role of KatG in Xcc physiology, we evaluated bacterial survival upon exposure to UV-A or UV-B radiation. In both conditions, XcckatG showed a high mortality in comparison to Xcc wild-type. Finally, we studied the development of bacterial biofilms. While structured biofilms were observed for the Xcc wild-type, the development of these structures was impaired for XcckatG. Based on these results, we demonstrated that KatG is responsible for Xcc adaptive response to H2O2 and a key component of the bacterial response to oxidative stress. Moreover, this enzyme plays an important role during Xcc epiphytic survival, being essential for biofilm formation and UV resistance.es
dc.descriptionPara citar este articulo: Tondo ML, Delprato ML, Kraiselburd I, Fernández Zenoff MV, Farías ME, Orellano EG (2016) KatG, the Bifunctional Catalase of Xanthomonas citri subsp. citri, Responds to Hydrogen Peroxide and Contributes to Epiphytic Survival on Citrus Leaves. PLoS ONE 11(3): e0151657. doi:10.1371/journal.pone.0151657
dc.description.filFil: Tondo, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Delprato, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Kraiselburd, Ivana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Fernández Zenoff, María Verónica. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI -CONICET); Argentina.es
dc.description.filFil: Farías, María Eugenia. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI -CONICET); Argentina.es
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2010-1762es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 00873es
dc.formatapplication/pdf
dc.format.extent1-19es
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/2133/19678
dc.language.isoenges
dc.publisherPublic Library of Science (PLOS)es
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0151657es
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0151657es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderTondo, María Lauraes
dc.rights.holderDelprato, María Lauraes
dc.rights.holderKraiselburd, Ivanaes
dc.rights.holderFernández Zenoff, María Verónicaes
dc.rights.holderFarías, María Eugeniaes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectXanthomonas citri subsp. citries
dc.subjectHydrogen Peroxidees
dc.subjectKatGes
dc.subjectCatalasees
dc.subjectPlant Leaveses
dc.subjectEpiphytic Survivales
dc.subjectCitrus Cankeres
dc.titleKatG, the bifunctional catalase of xanthomonas citri subsp. citri, responds to hydrogen peroxide and contributes to epiphytic survival on citrus leaveses
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
620.PDF
Tamaño:
2.17 MB
Formato:
Adobe Portable Document Format
Descripción:
versión post-print
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.59 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Departamento de Ciencias Biológicas - Artículo de Revista