Organic anion transporter 5 renal expression and urinary excretion in rats with vascular calcification

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dc.citation.titleBioMed Research International
dc.creatorHazelhoff, María Herminia
dc.creatorBulacio, Romina Paula
dc.creatorTorres, Adriana Mónica
dc.date.accessioned2024-10-15T16:30:33Z
dc.date.available2024-10-15T16:30:33Z
dc.date.issued2013
dc.description.abstractIt has been described renal damage in rats with vascular calcification. The organic anion transporter 5 (Oat5) is only expressed in kidney, and its urinary excretion was proposed as potential early biomarker of renal injury. The aim of this study was to evaluate the Oat5 renal expression and its urinary excretion in an experimental model of vascular calcification in comparison with traditional markers of renal injury. Vascular calcification was obtained by the administration of an overdose of vitamin D3 (300,000 IU/kg, b.w., i.m.) to male Wistar rats. Oat5 urinary abundance was evaluated by Western blotting. Traditional markers of renal injury, such as creatinine and urea plasma levels, urinary protein levels, and urinary alkaline phosphatase (AP) activity, were determined using commercial kits. Histology was assessed by hematoxylin/eosin staining. Oat5 renal expression was evaluated by Western blotting and by immunohistochemistry. An increased expression of Oat5 in renal homogenates, in apical membranes, and in its urinary excretion was observed in rats with vascular calcification. The traditional parameters used to evaluate renal function were not modified, with the exception of histology. It is possible to postulate the urinary excretion of Oat5 as a potential noninvasive biomarker of renal injury associated with vascular calcification.
dc.description.filFil: Hazelhoff, María Herminia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Farmacología; Argentina.
dc.description.filFil: Bulacio, Romina Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Farmacología; Argentina.
dc.description.filFil: Torres, Adriana Mónica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Farmacología; Argentina
dc.description.sponsorshipFondo para la Investigación Científica y Tecnológica (FONCYT)
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipUniversidad Nacional de Rosario (UNR)
dc.description.sponsorshipSecretaría de Estado de Ciencia, Tecnología e Innovación de la Provincia de Santa Fe (SECTeI)
dc.format.extent1-10
dc.identifier.issn2314-6141
dc.identifier.urihttps://hdl.handle.net/2133/27950
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1155/2013/283429
dc.relation.publisherversionhttp://dx.doi.org/10.1155/2013/283429
dc.rightsopenAccess
dc.rights.holderHazelhoff, María Herminia
dc.rights.holderBulacio, Romina Paula
dc.rights.holderTorres, Adriana Mónica
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectVascular calcification
dc.subjectAlkaline phosphatase
dc.subjectVitamin D
dc.subjectCreatinine
dc.subjectUrea
dc.subjectAorta abdominal
dc.subjectImmunohistochemistry
dc.titleOrganic anion transporter 5 renal expression and urinary excretion in rats with vascular calcification
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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