Progression of Trypanosoma cruzi Dm28c strain infection in a BALB/c mouse experimental model

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dc.citation.titleParasitologia
dc.citation.volume5
dc.creatorDe Hernández, María Azul
dc.creatorVillar, Silvina Raquel
dc.creatorCribb, Pamela
dc.date.accessioned2025-09-17T12:48:19Z
dc.date.available2025-09-17T12:48:19Z
dc.date.issued2025-09-09
dc.description.abstractChagas disease, caused by Trypanosoma cruzi, presents a variety of clinical outcomes ranging from mild symptoms to Chagas cardiomyopathy, the most severe and life-threatening manifestation of the disease. The degree of virulence is influenced by both parasite and host factors. In this study, we characterized a murine infection model using the T. cruzi Dm28c strain in BALB/c mice to assess disease progression. Infected mice showed a peak of parasitemia at 14 dpi, followed by a progressive decrease. Spleen weight increased up to sixfold compared to uninfected controls at 14 and 21 dpi, correlating with parasitemia levels. Histological analysis revealed focal inflammatory infiltrates in the heart starting at 7 dpi, with maximal intensity at 14 and 21 dpi. The expression of inflammatory cytokines (IFN-γ, IL-1β, TNF-α) and anti-inflammatory cytokines (IL-10, TGF-β) in the spleen showed a dynamic profile, with an early increase during the acute phase. Dm28c infection of BALB/c mice can be considered as a non-lethal Chagas disease experimental model, with detectable parasitemia during the acute phase and a controlled inflammatory response.
dc.description.filFil: De Hernández, María Azul. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Villar, Silvina Raquel. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Centro de Investigación y Producción de Reactivos Biológicos; Argentina.
dc.description.filFil: Villar, Silvina Raquel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Inmunología Clínica y Experimental de Rosario (IDICER-CONICET); Argentina.
dc.description.filFil: Cribb, Pamela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 2016-0439, PICT 2019-4212
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 2021-0848
dc.description.versionpeerreviewed
dc.format.extent1-12
dc.identifier.issn2673-6772
dc.identifier.urihttps://hdl.handle.net/2133/30325
dc.language.isoen
dc.publisherMDPI
dc.relation.publisherversionhttps://doi.org/10.3390/parasitologia5030047
dc.relation.publisherversionhttps://www.mdpi.com/2673-6772/5/3/47
dc.rightsopenAccess
dc.rights.holderDe Hernández, María Azul
dc.rights.holderVillar, Silvina Raquel
dc.rights.holderCribb, Pamela
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTrypanosoma
dc.subjectInflammatory response
dc.subjectParasitemia
dc.subjectNon-lethal
dc.subjectSpleen
dc.titleProgression of Trypanosoma cruzi Dm28c strain infection in a BALB/c mouse experimental model
dc.typearticulo
dc.type.versionpublishedVersion

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(FBIOyF) Departamento de Microbiología - Artículos