Genomic comparative analysis of the environmental Enterococcus mundtii against enterococcal representative species

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dc.citation.titleBMC Genomics
dc.citation.volume15
dc.creatorRepizo, Guillermo Daniel
dc.creatorEspariz, Martín
dc.creatorBlancato, Víctor Sebastián
dc.creatorSuárez, Cristian Alejandro
dc.creatorEsteban, Luis
dc.creatorMagni, Christian
dc.date.accessioned2024-08-23T15:04:59Z
dc.date.available2024-08-23T15:04:59Z
dc.date.issued2014-06-18
dc.description.abstractBackground Enterococcus mundtii is a yellow-pigmented microorganism rarely found in human infections. The draft genome sequence of E. mundtii was recently announced. Its genome encodes at least 2,589 genes and 57 RNAs, and 4 putative genomic islands have been detected. The objective of this study was to compare the genetic content of E. mundtii with respect to other enterococcal species and, more specifically, to identify genes coding for putative virulence traits present in enterococcal opportunistic pathogens. Results An in-depth mining of the annotated genome was performed in order to uncover the unique properties of this microorganism, which allowed us to detect a gene encoding the antimicrobial peptide mundticin among other relevant features. Moreover, in this study a comparative genomic analysis against commensal and pathogenic enterococcal species, for which genomic sequences have been released, was conducted for the first time. Furthermore, our study reveals significant similarities in gene content between this environmental isolate and the selected enterococci strains (sharing an “enterococcal gene core” of 805 CDS), which contributes to understand the persistence of this genus in different niches and also improves our knowledge about the genetics of this diverse group of microorganisms that includes environmental, commensal and opportunistic pathogens. Conclusion Although E. mundtii CRL1656 is phylogenetically closer to E. faecium, frequently responsible of nosocomial infections, this strain does not encode the most relevant relevant virulence factors found in the enterococcal clinical isolates and bioinformatic predictions indicate that it possesses the lowest number of putative pathogenic genes among the most representative enterococcal species. Accordingly, infection assays using the Galleria mellonella model confirmed its low virulence.
dc.description.filFil: Repizo, Guillermo Daniel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas Instituto de Biología Molecular y Celular de Rosario. Departamento de Microbiología (IBR-CONICET); Argentina.
dc.description.filFil: Espariz, Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas Instituto de Biología Molecular y Celular de Rosario. Departamento de Microbiología (IBR-CONICET); Argentina.
dc.description.filFil: Blancato, Víctor Sebastián. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas Instituto de Biología Molecular y Celular de Rosario. Departamento de Microbiología (IBR-CONICET); Argentina.
dc.description.filFil: Suárez, Cristian Alejandro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas Instituto de Biología Molecular y Celular de Rosario. Departamento de Microbiología (IBR-CONICET); Argentina.
dc.description.filFil: Esteban, Luis. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Departamento de Fisiología; Argentina.
dc.description.filFil: Magni, Christian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas Instituto de Biología Molecular y Celular de Rosario. Departamento de Microbiología (IBR-CONICET); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT2010-1828 , PIP 2012/14
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.format.extent1-13
dc.identifier.issn1471-2164
dc.identifier.urihttps://hdl.handle.net/2133/27634
dc.language.isoen
dc.publisherBMC
dc.relation.publisherversionhttps://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-489
dc.relation.publisherversionhttps://doi.org/10.1186/1471-2164-15-489
dc.rightsopenAccess
dc.rights.holderRepizo, Guillermo Daniel
dc.rights.holderEspariz, Martín
dc.rights.holderBlancato, Víctor Sebastián
dc.rights.holderSuárez, Cristian Alejandro
dc.rights.holderEsteban, Luis
dc.rights.holderMagni, Christian
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEnterococcus
dc.subjectEnterococcus mundtii
dc.subjectGenomic comparative analysis
dc.titleGenomic comparative analysis of the environmental Enterococcus mundtii against enterococcal representative species
dc.typearticulo
dc.type.versionpublishedVersion

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