Effects of lactoferrin, a protein present in the female reproductive tract, on parameters of human sperm capacitation and gamete interaction

Vista sencilla de ítem
dc.citation.titleAndrology
dc.citation.volume3
dc.creatorZumoffen, Carlos María
dc.creatorMassa, Estefanía
dc.creatorCaille, Adriana María
dc.creatorMunuce, María José
dc.creatorGhersevich, Sergio Albino
dc.date.accessioned2024-12-03T12:26:17Z
dc.date.available2024-12-03T12:26:17Z
dc.date.issued2015-10-07
dc.description.abstractIn a recent study, lactoferrin (LF) was detected in human oviductal secretion. The protein was able to bind to oocytes and sperm, and modulated gamete interaction. The aim of the present study was to investigate the effect of LF on parameters related to human sperm capacitation and sperm–zona pellucida interaction. Semen samples were obtained from healthy normozoospermic donors (n = 7). Human follicular fluids and oocytes were collected from patients undergoing in vitro fertilization. Motile sperm obtained by swim-up were incubated for 6 or 22 h under capacitating conditions with LF (0–100 μg/mL). After incubations, viability, motility, presence of α-d-mannose receptors (using a fluorescent probe on mannose coupled to bovine serum albumin), spontaneous and induced acrosome reaction (assessed with Pisum sativum agglutinin conjugated to fluorescein isothiocyanate), and tyrosine phosphorylation of sperm proteins were evaluated. Sperm–zona pellucida interaction in the presence of LF was investigated using the hemizone assay. The presence of LF did not affect sperm viability or motility, but caused a dose-dependent significant decrease in sperm α-d-mannose-binding sites, and the effect was already significant with the lowest concentration of the protein used after 22 h incubation. Dose-dependent significant increases in both induced acrosome reaction and tyrosine phosphorylation of sperm proteins were observed in the presence of LF. The present data indicate that LF modulates parameters of sperm function. The inhibition of gamete interaction by LF could be partially explained by the decrease in sperm d-mannose-binding sites. The presence of the LF promoted sperm capacitation in vitro.
dc.description.filFil: Zumoffen, Carlos María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva. Área Bioquímica Clínica; Argentina.
dc.description.filFil: Massa, Estefanía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva. Área Bioquímica Clínica; Argentina.
dc.description.filFil: Caille, Adriana María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva. Área Bioquímica Clínica; Argentina.
dc.description.filFil: Munuce, María José. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva. Área Bioquímica Clínica; Argentina.
dc.description.filFil: Ghersevich, Sergio Albino. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva. Área Bioquímica Clínica; Argentina.
dc.description.sponsorshipSecretaría de Ciencia y Tecnología de la Universidad Nacional de Rosario: PID-BIO238
dc.description.versionpeerreviewed
dc.format.extent1-8
dc.identifier.e-issn2047-2927
dc.identifier.issn2047-2919
dc.identifier.urihttps://hdl.handle.net/2133/28294
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1111/andr.12093
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/andr.12093
dc.rightsopenAccess
dc.rights.holderZumoffen, Carlos María
dc.rights.holderMassa, Estefanía
dc.rights.holderCaille, Adriana María
dc.rights.holderMunuce, María José
dc.rights.holderGhersevich, Sergio Albino
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAcrosome
dc.subjectCapacitation
dc.subjectLactoferrin
dc.subjectReproductive tract
dc.subjectSperm function
dc.subjectSperm capacitation
dc.subjectSperm-Ovum interactions
dc.subjectAcrosome reaction
dc.titleEffects of lactoferrin, a protein present in the female reproductive tract, on parameters of human sperm capacitation and gamete interaction
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
File - Andrology - 2015 - Zumoffen - Effects of lactoferrin a protein present in the female reproductive tract on pa.pdf
Tamaño:
489.6 KB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.87 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Laboratorio de Medicina Reproductiva (LMR) - Artículo de Revista
(FBIOyF) Departamento de Bioquímica Clínica - Artículos