A serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrix

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dc.creatorGarcía, Lucila
dc.creatorMolina, María Celeste
dc.creatorPadgett Pagliai, Kaylie Allyson
dc.creatorTorres, Pablo S.
dc.creatorBruna, Roberto Emanuel
dc.creatorGarcía Véscovi, Eleonora
dc.creatorGonzález, Claudio F.
dc.creatorGadea, José
dc.creatorMarano, María Rosa
dc.date.accessioned2022-12-15T19:22:37Z
dc.date.available2022-12-15T19:22:37Z
dc.date.issued2022-10
dc.descriptionHuanglongbing (HLB), the current major threat for Citrus species, is caused by intracellular alphaproteobacteria of the genus Candidatus Liberibacter (CaL), with CaL asiaticus (CLas) being the most prevalent species. This bacterium inhabits phloem cells and is transmitted by the psyllid Diaphorina citri. A gene encoding a putative serralysin-like metalloprotease (CLIBASIA_01345) was identified in the CLas genome. The expression levels of this gene were found to be higher in citrus leaves than in psyllids, suggesting a function for this protease in adaptation to the plant environment. Here, we study the putative role of CLas-serralysin (Las1345) as virulence factor. We first assayed whether Las1345 could be secreted by two different surrogate bacteria, Rhizobium leguminosarum bv. viciae A34 (A34) and Serratia marcescens. The protein was detected only in the cellular fraction of A34 and S. marcescens expressing Las1345, and increased protease activity of those bacteria by 2.55 and 4.25- fold, respectively. In contrast, Las1345 expressed in Nicotiana benthamiana leaves did not show protease activity nor alterations in the cell membrane, suggesting that Las1345 do not function as a protease in the plant cell. Las1345 expression negatively regulated cell motility, exopolysaccharide production, and biofilm formation in Xanthomonas campestris pv. campestris (Xcc). This bacterial phenotype was correlated with reduced growth and survival on leaf surfaces as well as reduced disease symptoms in N. benthamiana and Arabidopsis. These results support a model where Las1345 could modify extracellular components to adapt bacterial shape and appendages to the phloem environment, thus contributing to virulence.es
dc.description.filFil: García, Lucila. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: García, Lucila. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.filFil: Molina, María Celeste. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: Molina, María Celeste. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.filFil: Padgett Pagliai, Kaylie Allyson. University of Florida. Genetics Institute, Institute of Food and Agricultural Sciences. Department of Microbiology and Cell Science; United States.
dc.description.filFil: Torres, Pablo S. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: Torres, Pablo S. Universidad Politécnica de Valencia. Instituto de Biología Molecular y Celular de Plantas (IBMCP); Spain.
dc.description.filFil: Bruna, Roberto Emanuel. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: García Véscovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: González, Claudio F. University of Florida. Genetics Institute, Institute of Food and Agricultural Sciences. Department of Microbiology and Cell Science; United States.
dc.description.filFil: Gadea, José. Universidad Politécnica de Valencia. Instituto de Biología Molecular y Celular de Plantas (IBMCP); Spain.
dc.description.filFil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: Marano, María Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.sponsorshipEstación Experimental Agropecuaria Montecarlo
dc.description.sponsorshipInstituto Nacional de Tecnología Agropecuaria (INTA)
dc.formatapplication/pdf
dc.format.extent1-19
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/2133/25033
dc.language.isoenges
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2022.1006962
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2022.1006962/full
dc.rightsopenAccesses
dc.rights.holderGarcía, Lucilaes
dc.rights.holderMolina, María Celestees
dc.rights.holderPadgett-Pagliai, Kaylie Allysones
dc.rights.holderTorres, Pablo S.es
dc.rights.holderBruna, Roberto Emanueles
dc.rights.holderGarcía Véscovi, Eleonoraes
dc.rights.holderGonzález, Claudio F.es
dc.rights.holderGadea, Josées
dc.rights.holderMarano, María Rosaes
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectBiofilmes
dc.subjectHuanglongbinges
dc.subjectProteasees
dc.subjectSurrogate bacteriaes
dc.subjectVirulence factores
dc.titleA serralysin-like protein of Candidatus Liberibacter asiaticus modulates components of the bacterial extracellular matrixes
dc.typearticle
dc.typeartículo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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