Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus

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dc.citation.titleEnvironmental Technology & Innovationes
dc.citation.volume26es
dc.creatorPellieri, Carlos Martín
dc.creatorTaddia, Antonela
dc.creatorLoureiro, Dana Belén
dc.creatorBortolato, Santiago Andrés
dc.creatorTubio, Gisela
dc.date.accessioned2022-04-08T14:45:23Z
dc.date.available2022-04-08T14:45:23Z
dc.date.issued2022-01-24
dc.descriptionHemicellulose is a major component of plant cell walls and xylan is the most predominant polysaccharide. Xylan degrading enzymes integrate the xylanolytic system. Xylanolytic enzymes were produced by fermentation by Aspergillus niger and Thermomyces lanuginosus grown on grass considered unsuitable for farming called Spartina argentinensis. Significant parameters: type of fermentation used (SmF or SSF), type of leaves of S. argentinensis (green or senescent) and conidia final concentration for xylanase production were screened and optimized. The main results showed that the highest levels of xylanolytic enzyme production were obtained by 1 × 105 conidia/mL of A. niger in SmF at 96 h, 30ºC, with a mixture of 20.20% of senescent and 79.80% of green leaves. The xylanase specific activity obtained was 62 U/mg, higher than the activity obtained (23 U/mg) in previous work (Taddia et al., 2019), and the concentration of xylanolytic production over that of glucanase activity was maximized five times. The optimized enzymatic extract obtained was characterized by LC-MS and HPLC of carbohydrates. Six enzymes were identified as constituents of the xylanolytic complex and seven carbohydrates. Moreover, the xylanolytic enzyme extract was stable for 30 days at 20 °C. Thus, S. argentinensis can be used within the framework of a circular economy, rendering a synergistic combination of the xylanolytic enzymes with industrial applications.es
dc.description.filFil: Pellieri, Carlos Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROByQ-CONICET). Argentina.es
dc.description.filFil: Taddia, Antonela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROByQ-CONICET). Argentina.es
dc.description.filFil: Loureiro, Dana Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROByQ-CONICET). Argentina.es
dc.description.filFil: Bortolato, Santiago Andrés . Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química de Rosario (IQUIR-CONICET). Argentina.es
dc.description.filFil: Tubio, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROByQ-CONICET). Argentina.es
dc.description.sponsorshipMinisterio de Ciencia, Tecnología e Innovación Productiva de Santa Fe: IO2018009
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PUE BD20160041CO
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica: PICT-II-B-2018 2054
dc.description.sponsorshipSecretaría de Ciencia y Tecnología de la Universidad Nacional de Rosario: 80020180100033UR
dc.description.sponsorshipSecretaría de Ciencia y Tecnología de la Universidad Nacional de Rosario: 80020180100036UR
dc.formatapplication/pdf
dc.format.extent1-14es
dc.identifier.issn2352-1864es
dc.identifier.urihttp://hdl.handle.net/2133/23373
dc.language.isoenges
dc.publisherElsevieres
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2352186422000165?via%3Dihubes
dc.rightsopenAccesses
dc.rights.holderPellieri, Carlos Martínes
dc.rights.holderTaddia, Antonelaes
dc.rights.holderLoureiro, Dana Belénes
dc.rights.holderBortolato, Santiago Andréses
dc.rights.holderTubio, Giselaes
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBioprocesseses
dc.subjectLignocellulosic Biomasses
dc.subjectFungal Fermentationes
dc.subjectEnzyme Statistical Design Productiones
dc.subjectSustainabilityes
dc.subjectCircular Economyes
dc.titleSpartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous funguses
dc.typearticle
dc.typepublishedVersion
dc.typeartículo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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