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Ítem Acceso Abierto An industrial scale process for the enzymatic removal of steryl glucosides from biodiesel(BMC (part of Springer Nature), 2015-12-21) Peirú, Salvador; Aguirre, Andrés; Eberhardt, María Florencia; Braia, Mauricio Javier; Cabrera, Rodolfo; Menzella, Hugo G.Background: Biodiesels produced from transesterification of vegetable oils have a major quality problem due to the presence of precipitates, which need to be removed to avoid clogging of filters and engine failures. These precipitates have been reported to be mostly composed of steryl glucosides (SGs), but so far industrial cost-effective methods to remove these compounds are not available. Here we describe a novel method for the efficient removal of SGs from biodiesel, based on the hydrolytic activity of a thermostable β-glycosidase obtained from Thermococcus litoralis. Results: A steryl glucosidase (SGase) enzyme from T. litoralis was produced and purified from Escherichia coli cultures expressing a synthetic gene, and used to treat soybean-derived biodiesel. Several optimization steps allowed for the selection of optimal reaction conditions to finally provide a simple and efficient process for the removal of SGs from crude biodiesel. The resulting biodiesel displayed filterability properties similar to distilled biodiesel according to the total contamination (TC), the cold soak filtration test (CSFT), filter blocking tendency (FBT), and cold soak filter blocking tendency (CSFBT) tests. The process was successfully scaled up to a 20 ton reactor, confirming its adaptability to industrial settings. Conclusions: The results presented in this work provide a novel path for the removal of steryl glucosides from biodiesel using a cost-effective, environmentally friendly and scalable enzymatic process, contributing to the adoption of this renewable fuel.Ítem Acceso Abierto Expression of codon optimized genes in microbial systems: current industrial applications and perspectives(Frontiers Media, 2014-02-14) Elena, Claudia; Ravasi, Pablo; Castelli, María Eugenia; Peirú, Salvador ; Menzella, Hugo G.The efficient production of functional proteins in heterologous hosts is one of the major bases of modern biotechnology. Unfortunately, many genes are difficult to express outside their original context. Due to their apparent “silent” nature, synonymous codon substitutions have long been thought to be trivial. In recent years, this dogma has been refuted by evidence that codon replacement can have a significant impact on gene expression levels and protein folding. In the past decade, considerable advances in the speed and cost of gene synthesis have facilitated the complete redesign of entire gene sequences, dramatically improving the likelihood of high protein expression. This technology significantly impacts the economic feasibility of microbial-based biotechnological processes by, for example, increasing the volumetric productivities of recombinant proteins or facilitating the redesign of novel biosynthetic routes for the production of metabolites. This review discusses the current applications of this technology, particularly those regarding the production of small molecules and industrially relevant recombinant enzymes. Suggestions for future research and potential uses are provided as well.Ítem Acceso Abierto Spartina argentinensis valorization and process optimization for enhanced production of hydrolytic enzymes by filamentous fungus(Elsevier, 2022-01-24) Pellieri, Carlos Martín; Taddia, Antonela; Loureiro, Dana Belén; Bortolato, Santiago Andrés; Tubio, GiselaÍtem Acceso Abierto Insights on fungal solid-state fermentation for waste valorization: Conidia and chitinase production in different reactor configurations(Elsevier, 2022-02-07) Sala, A.; Echegaray, T.; Palomas, G.; Boggione, María Julia; Tubio, Gisela; Barrena, R.; Artola, A.Ítem Acceso Abierto Correction to: Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain(Springer, 2021) Cerminati, Sebastián; Leroux, Mélanie; Anselmi, Pablo Ariel; Peirú, Salvador; Alonso, Juan C.; Priem, Bernard; Menzella, Hugo G.Ítem Acceso Abierto Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain(Springer, 2021-04-05) Cerminati, Sebastián; Leroux, Mélanie; Anselmi, Pablo Ariel; Peirú, Salvador; Alonso, Juan C.; Priem, Bernard; Menzella, Hugo G.