Photosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotype

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dc.citation.titleBiochimica et Biophysica Acta (BBA) - Bioenergeticses
dc.citation.volume1861 (8)es
dc.creatorGómez, Rodrigo Lionel
dc.creatorFigueroa, Nicolás
dc.creatorMelzer, Michael
dc.creatorHajirezaei, Mohammad-Reza
dc.creatorCarrillo, Néstor
dc.creatorLodeyro, Anabella F.
dc.date.accessioned2020-08-14T14:38:56Z
dc.date.available2020-08-14T14:38:56Z
dc.date.issued2020-08-01
dc.descriptionFlavodoxins are electron carrier flavoproteins present in bacteria and photosynthetic microorganisms which duplicate the functional properties of iron-sulphur containing ferredoxins and replace them under adverse environmental situations that lead to ferredoxin decline. When expressed in plant chloroplasts, flavodoxin complemented ferredoxin deficiency and improved tolerance to multiple sources of biotic, abiotic and xenobiotic stress. Analysis of flavodoxin-expressing plants grown under normal conditions, in which the two carriers are present, revealed phenotypic effects unrelated to ferredoxin replacement. Flavodoxin thus provided a tool to alter the chloroplast redox poise in a customized way and to investigate its consequences on plant physiology and development. We describe herein the effects exerted by the flavoprotein on the function of the photosynthetic machinery. Pigment analysis revealed significant increases in chlorophyll a, carotenoids and chlorophyll a/b ratio in flavodoxin-expressing tobacco lines. Results suggest smaller antenna size in these plants, supported by lower relative contents of light-harvesting complex proteins. Chlorophyll a fluorescence and P700 spectroscopy measurements indicated that transgenic plants displayed higher quantum yields for both photosystems, a more oxidized plastoquinone pool under steady-state conditions and faster plastoquinone dark oxidation after a pulse of saturating light. Many of these effects resemble the phenotypes exhibited by leaves adapted to high irradiation, a most common environmental hardship faced by plants growing in the field. The results suggest that flavodoxin-expressing plants would be better prepared to cope with this adverse situation, and concur with earlier observations reporting that hundreds of stress-responsive genes were induced in the absence of stress in these lines.es
dc.description.filFil: Gómez, Rodrigo Lionel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Figueroa, Nicolás. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Melzer, Michael. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.es
dc.description.filFil: Hajirezaei, Mohammad-Reza. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.es
dc.description.filFil: Carrillo, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Lodeyro, Anabella F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT, Argentina): PICT 2015-3828es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT, Argentina): PICT 2017-3080es
dc.description.sponsorshipAgencia Santafesina de Ciencia, Tecnología e Innovación: IO-212-17es
dc.formatapplication/pdf
dc.format.extent148211es
dc.identifier.issn0005-2728es
dc.identifier.urihttp://hdl.handle.net/2133/18607
dc.language.isoenges
dc.publisherElsevieres
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S000527282030061X?via%3Dihub#ks0010es
dc.relation.publisherversionhttps://doi.org/10.1016/j.bbabio.2020.148211es
dc.rightsembargoedAccesses
dc.rights.holderGómez, Rodrigo Lioneles
dc.rights.holderFigueroa, Nicoláses
dc.rights.holderMelzer, Michaeles
dc.rights.holderHajirezaei, Mohammad-Rezaes
dc.rights.holderCarrillo, Néstores
dc.rights.holderLodeyro, Anabella F.es
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderElsevieres
dc.rights.textAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es*
dc.subjectPhotosynthetic electron transport chaines
dc.subjectPlastoquinone pooles
dc.subjectRedox statees
dc.subjectChloroplastes
dc.subjectHigh irradiationes
dc.subjectFlavodoxines
dc.titlePhotosynthetic characterization of flavodoxin-expressing tobacco plants reveals a high light acclimation-like phenotypees
dc.typearticle
dc.typeartículo
dc.typeacceptedVersion
dc.type.collectionarticulo
dc.type.versionacceptedVersiones

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