Construction of three new Gateway® expression plasmids for Trypanosoma cruzi

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dc.citation.titleMemórias do Instituto Oswaldo Cruz
dc.citation.volume109
dc.creatorAlonso, Victoria Lucía
dc.creatorRitagliati, Carla
dc.creatorCribb, Pamela
dc.creatorSerra, Esteban Carlos
dc.date.accessioned2024-06-05T20:09:33Z
dc.date.available2024-06-05T20:09:33Z
dc.date.issued2014-12
dc.description.abstractWe present here three expression plasmids for Trypanosoma cruzi adapted to the Gateway® recombination cloning system. Two of these plasmids were designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag). The TAPtag and Gateway® cassette were introduced into an episomal (pTEX) and an integrative (pTREX) plasmid. Both plasmids were assayed by introducing green fluorescent protein (GFP) by recombination and the integrity of the double-tagged protein was determined by western blotting and immunofluorescence microscopy. The third Gateway adapted vector assayed was the inducible pTcINDEX. When tested with GFP, pTcINDEX-GW showed a good response to tetracycline, being less leaky than its precursor (pTcINDEX).
dc.description.filFil: Alonso, Victoria Lucía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ritagliati, Carla. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Cribb, Pamela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Serra, Esteban Carlos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.versionpeerreviewed
dc.format.extent1-5
dc.identifier.e-issn1678-8060
dc.identifier.issn0074-0276
dc.identifier.urihttps://hdl.handle.net/2133/27228
dc.language.isoen
dc.publisherInstituto Oswaldo Cruz
dc.relation.publisherversionhttps://doi.org/10.1590/0074-0276140238
dc.relation.publisherversionhttps://www.scielo.br/j/mioc/a/ysmnG8sSGdVpRTXbJkdvBWc/?lang=en
dc.rightsopenAccess
dc.rights.holderAlonso, Victoria Lucía
dc.rights.holderRitagliati, Carla
dc.rights.holderCribb, Pamela
dc.rights.holderSerra, Esteban Carlos
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectTrypanosoma cruzi
dc.subjectExpression vectors
dc.subjectGateway® system
dc.subjectTandem affinity purification
dc.subjectpTcINDEX
dc.titleConstruction of three new Gateway® expression plasmids for Trypanosoma cruzi
dc.typearticulo
dc.type.versionpublishedVersion

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