Identification and characterization of novel alphacoronaviruses in Tadarida brasiliensis (Chiroptera, Molossidae) from Argentina: insights into recombination as a mechanism favoring bat coronavirus cross-species transmission

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dc.citation.titleMicrobiology Spectrum
dc.citation.volume1-19
dc.contributor.orcidhttps://orcid.org/0000-0003-4925-9075
dc.contributor.orcidhttps://orcid.org/0000-0001-6467-0650
dc.creatorCerri, Agustina
dc.creatorBolatti, Elisa María
dc.creatorZorec, Tomaz M.
dc.creatorMontani, Maria E.
dc.creatorRimondi, Agustina
dc.creatorHosnjak, Lea
dc.creatorCasal, Pablo E.
dc.creatorDi Domenica, Violeta
dc.creatorBarquez, Rubén M.
dc.creatorPoljak, Mario
dc.creatorGiri, Adriana Angélica
dc.date.accessioned2024-04-03T14:39:58Z
dc.date.available2024-04-03T14:39:58Z
dc.date.issued2023-09-11
dc.description.abstractBats are reservoirs of various coronaviruses that can jump between bat species or other mammalian hosts, including humans. This article explores coronavirus infection in three bat species (Tadarida brasiliensis, Eumops bonariensis, and Molossus molossus) of the family Molossidae from Argentina using whole viral metagenome analysis. Fecal samples of 47 bats from three semiurban or highly urbanized areas of the province of Santa Fe were investigated. After viral particle enrichment, total RNA was sequenced using the Illumina NextSeq 550 instrument; the reads were assembled into contigs and taxonomically and phylogenetically analyzed. Three novel complete Alphacoronavirus (AlphaCoV) genomes (Tb1–3) and two partial sequences were identified in T. brasiliensis (Tb4–5), and an additional four partial sequences were identified in M. molossus (Mm1–4). Phylogenomic analysis showed that the novel AlphaCoV clustered in two different lineages distinct from the 15 officially recognized AlphaCoV subgenera. Tb2 and Tb3 isolates appeared to be variants of the same virus, probably involved in a persistent infectious cycle within the T. brasiliensis colony. Using recombination analysis, we detected a statistically significant event in Spike gene, which was reinforced by phylogenetic tree incongruence analysis, involving novel Tb1 and AlphaCoVs identified in Eptesicus fuscus (family Vespertilionidae) from the U.S. The putative recombinant region is in the S1 subdomain of the Spike gene, encompassing the potential receptor-binding domain of AlphaCoVs. This study reports the firstfirstAlphaCoV genomes in molossids from the Americas and provides new insights into recombination as an important mode of evolution of coronaviruses involved in cross-species transmission.
dc.description.filFil: Cerri, Agustina. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Grupo Virología Humana; Argentina.
dc.description.filFil: Bolatti, Elisa María. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Grupo Virología Humana; Argentina.
dc.description.filFil: Bolatti, Elisa María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.filFil: Zorec, Tomaz M. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia.
dc.description.filFil: Montani, María E. Museo Provincial de Ciencias Naturales “Dr. Ángel Gallardo”; Argentina.
dc.description.filFil: Montani, María E. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Programa de Investigaciones de Biodiversidad Argentina (PIDBA); Argentina.
dc.description.filFil: Montani, María E. Programa de Conservación de los Murciélagos de Argentina (PCMA); Argentina.
dc.description.filFil: Rimondi, Agustina. Instituto de Virología e Innovaciones tecnológicas (IVIT-INTA-CONICET); Argentina.
dc.description.filFil: Rimondi, Agustina. Robert Koch Institute; Germany.
dc.description.filFil: Hosnjak, Lea. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia.
dc.description.filFil: Casal, Pablo E. DETx MOL S.A; Argentina.
dc.description.filFil: Di Domenica, Violeta. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Grupo Virología Humana; Argentina.
dc.description.filFil: Di Domenica, Violeta. Programa de Conservación de los Murciélagos de Argentina (PCMA); Argentina.
dc.description.filFil: Barquez, Rubén M. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Programa de Investigaciones de Biodiversidad Argentina (PIDBA); Argentina.
dc.description.filFil: Barquez, Rubén M. Programa de Conservación de los Murciélagos de Argentina (PCMA); Argentina.
dc.description.filFil: Poljak, Mario. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia.
dc.description.filFil: Giri, Adriana Angélica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Grupo Virología Humana; Argentina.
dc.description.filFil: Giri, Adriana Angélica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT-2019–01790, PICT-2020–00571
dc.description.sponsorshipEuropean Society of Clinical Microbiology and Infectious Diseases (ESCMID) Observership Program
dc.description.sponsorshipSlovenian Research Agency: grant P3-00083
dc.description.versionpeerreviewed
dc.identifier.e-issn2165-0497
dc.identifier.urihttps://hdl.handle.net/2133/26839
dc.language.isoen
dc.publisherASM
dc.relation.publisherversionhttps://doi.org/10.1128/spectrum.02047-23
dc.relation.publisherversionhttps://journals.asm.org/doi/10.1128/spectrum.02047-23
dc.rightsopenAccess
dc.rights.holderCerri, Agustina
dc.rights.holderBolatti, Elisa María
dc.rights.holderZorec, Tomaz M.
dc.rights.holderMontani, María E.
dc.rights.holderRimondi, Agustina
dc.rights.holderHosnjak, Lea
dc.rights.holderCasal, Pablo E.
dc.rights.holderDi Domenica, Violeta
dc.rights.holderBarquez, Rubén M.
dc.rights.holderPoljak, Mario
dc.rights.holderGiri, Adriana Angélica
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBats
dc.subjectGenus alphacoronavirus
dc.subjectNovel genomes
dc.subjectRecombination
dc.subjectMolossidae
dc.subjectCross-species transmission
dc.subjectAmericas
dc.titleIdentification and characterization of novel alphacoronaviruses in Tadarida brasiliensis (Chiroptera, Molossidae) from Argentina: insights into recombination as a mechanism favoring bat coronavirus cross-species transmission
dc.typearticulo
dc.type.versionpublishedVersion

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