Genome comparison of two Exiguobacterium strains from high altitude andean lakes with different arsenic resistance: identification and 3D modeling of the Acr3 efflux pump

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dc.citation.titleFrontiers in Environmental Sciencees
dc.citation.volume3es
dc.creatorOrdoñez, Omar F.
dc.creatorLanzarotti, Esteban
dc.creatorKurth, Daniel
dc.creatorCortez, Néstor
dc.creatorFarías, María E.
dc.creatorTurjanski, Adrian G.
dc.date.accessioned2020-12-17T13:37:11Z
dc.date.available2020-12-17T13:37:11Z
dc.date.issued2015-07-22
dc.descriptionArsenic exists in natural systems in a variety of chemical forms, including inorganic arsenite (As [III]) and arsenate (As [V]). The majority of living organisms have evolved various mechanisms to avoid occurrence of arsenic inside the cell due to its toxicity. Common core genes include a transcriptional repressor ArsR, an arsenate reductase ArsC, and arsenite efflux pumps ArsB and Acr3. To understand arsenic resistance we have performed arsenic tolerance studies, genomic and bioinformatic analysis of two Exiguobacterium strains, S17 and N139, from the high-altitude Andean Lakes. In these environments high concentrations of arsenic were described in the water due to a natural geochemical phenomenon, therefore, these strains represent an attractive model system for the study of environmental stress and can be readily cultivated. Our experiments show that S17 has a greater tolerance to arsenite (10 mM) than N139, but similar growth in arsenate (150 mM). We sequenced the genome of the two Exiguobacterium and identified an acr3 gene in S17 as the only difference between both species regarding known arsenic resistance genes. To further understand the Acr3 we modeled the 3D structure and identified the location of relevant residues of this protein. Our model is in agreement with previous experiments and allowed us to identify a region where a relevant cysteine lies. This Acr3 membrane efflux pump, present only in S17, may explain its increased tolerance to As(III) and is the first Acr3-family protein described in Exiguobacterium genus.es
dc.description.filFil: Ordoñez, Omar F. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI). Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (CONICET); Argentina.es
dc.description.filFil: Lanzarotti, Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Física de los Materiales, Medio Ambiente y Energía (INQUIMAE-CONICET); Argentina.es
dc.description.filFil: Kurth, Daniel. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI). Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (CONICET); Argentina.es
dc.description.filFil: Cortez, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Farías, María E. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI). Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (CONICET); Argentina.es
dc.description.filFil: Turjanski, Adrian G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Física de los Materiales, Medio Ambiente y Energía (INQUIMAE-CONICET); Argentina.es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 0365/12es
dc.description.sponsorshipFondo para la Investigación Científica y Tecnológica (FONCYT): PICT 1788 y 2805es
dc.formatapplication/pdf
dc.format.extent1-12es
dc.identifier.issn2296-665Xes
dc.identifier.urihttp://hdl.handle.net/2133/19499
dc.language.isoenges
dc.publisherFrontierses
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fenvs.2015.00050/full#h8es
dc.relation.publisherversionhttps://doi.org/10.3389/fenvs.2015.00050es
dc.rightsopenAccesses
dc.rights.holderOrdoñez, Omar F.es
dc.rights.holderLanzarotti, Estebanes
dc.rights.holderKurth, Danieles
dc.rights.holderCortez, Néstores
dc.rights.holderFarías, María E.es
dc.rights.holderTurjanski, Adrian G.es
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectArsenic resistancees
dc.subjectExiguobacterium,es
dc.subjectMembrane proteinses
dc.subjectSequencinges
dc.subjectACR3es
dc.titleGenome comparison of two Exiguobacterium strains from high altitude andean lakes with different arsenic resistance: identification and 3D modeling of the Acr3 efflux pumpes
dc.typepublishedVersion
dc.typearticle
dc.typeartículo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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