A cAMP/CRP-controlled mechanism for the incorporation of extracellular ADP-glucose in Escherichia coli involving NupC and NupG nucleoside transporters

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dc.citation.titleScientific Reports
dc.creatorAlmagro, Goizeder
dc.creatorViale, Alejandro M.
dc.creatorMontero, Manuel
dc.creatorMuñoz, Francisco José
dc.creatorBaroja Fernández, Edurne
dc.creatorMori, Hirotada
dc.creatorPozueta Romero, Javier
dc.date.accessioned2024-10-08T18:16:16Z
dc.date.available2024-10-08T18:16:16Z
dc.date.issued2018-10-19
dc.description.abstractADP-glucose is the precursor of glycogen biosynthesis in bacteria, and a compound abundant in the starchy plant organs ingested by many mammals. Here we show that the enteric species Escherichia coli is capable of scavenging exogenous ADP-glucose for use as a glycosyl donor in glycogen biosynthesis and feed the adenine nucleotide pool. To unravel the molecular mechanisms involved in this process, we screened the E. coli single-gene deletion mutants of the Keio collection for glycogen content in ADP-glucose-containing culture medium. In comparison to wild-type (WT) cells, individual ∆nupC and ∆nupG mutants lacking the cAMP/CRP responsive inner-membrane nucleoside transporters NupC and NupG displayed reduced glycogen contents and slow ADP-glucose incorporation. In concordance, ∆cya and ∆crp mutants accumulated low levels of glycogen and slowly incorporated ADP-glucose. Two-thirds of the glycogen-excess mutants identifed during screening lacked functions that underlie envelope biogenesis and integrity, including the RpoE specifc RseA anti-sigma factor. These mutants exhibited higher ADP-glucose uptake than WT cells. The incorporation of either ∆crp, ∆nupG or ∆nupC null alleles sharply reduced the ADP-glucose incorporation and glycogen content initially witnessed in ∆rseA cells. Overall, the data showed that E. coli incorporates extracellular ADP-glucose through a cAMP/ CRP-regulated process involving the NupC and NupG nucleoside transporters that is facilitated under envelope stress conditions.
dc.description.filFil: Almagro, Goizeder. Instituto de Agrobiotecnología; Spain.
dc.description.filFil: Viale, Alejandro M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Departamento de Microbiología; Argentina.
dc.description.filFil: Montero, Manuel. Instituto de Agrobiotecnología; Spain.
dc.description.filFil: Muñoz, Francisco José. Instituto de Agrobiotecnología; Spain.
dc.description.filFil: Baroja Fernández, Edurne. Instituto de Agrobiotecnología; Spain.
dc.description.filFil: Mori, Hirotada. Nara Institute of Science and Technology. Data Science Center. Division of Biological Science; Japan.
dc.description.filFil: Pozueta Romero, Javier. Instituto de Agrobiotecnología; Spain.
dc.description.sponsorshipComisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional: BIO2013-49125-C2-1-P, BIO2016-78747-P
dc.format.extent1-15
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/2133/27942
dc.language.isoen
dc.publisherNature Research
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-018-33647-w
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-018-33647-w
dc.rightsopenAccess
dc.rights.holderAlmagro, Goizeder
dc.rights.holderViale, Alejandro M.
dc.rights.holderMontero, Manuel
dc.rights.holderMuñoz, Francisco José
dc.rights.holderBaroja Fernández, Edurne
dc.rights.holderMori, Hirotada
dc.rights.holderPozueta Romero, Javier
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEscherichia coli
dc.subjectAdenine nucleotides
dc.subjectAdenosine diphosphate glucose
dc.subjectNucleoside transport proteins
dc.subjectStress physiologique
dc.subjectAdenosine diphosphate
dc.titleA cAMP/CRP-controlled mechanism for the incorporation of extracellular ADP-glucose in Escherichia coli involving NupC and NupG nucleoside transporters
dc.typearticulo
dc.type.versionpublishedVersion

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