Cloning and characterization of the Type I Baeyer–Villiger monooxygenase from Leptospira biflexa

Vista sencilla de ítem
dc.citation.titleAMB Expresses
dc.citation.volume7es
dc.creatorCeccoli, Romina Denis
dc.creatorBianchi, Dario A.
dc.creatorFink, Michael J.
dc.creatorMihovilovic, Marko D.
dc.creatorRial, Daniela V.
dc.date.accessioned2020-12-17T13:02:00Z
dc.date.available2020-12-17T13:02:00Z
dc.date.issued2017-04-27
dc.descriptionBaeyer–Villiger monooxygenases are recognized by their ability and high selectivity as oxidative biocatalysts for the generation of esters or lactones using ketones as starting materials. These enzymes represent valuable tools for biooxidative syntheses since they can catalyze reactions that otherwise involve strong oxidative reagents. In this work, we present a novel enzyme, the Type I Baeyer–Villiger monooxygenase from Leptospira biflexa. This protein is phylogenetically distant from other well-characterized BVMOs. In order to study this new enzyme, we cloned its gene, expressed it in Escherichia coli and characterized the substrate scope of the Baeyer–Villiger monooxygenase from L. biflexa as a whole-cell biocatalyst. For this purpose, we performed the screening of a collection of ketones with variable structures and sizes, namely acyclic ketones, aromatic ketones, cyclic ketones, and fused ketones. As a result, we observed that this biocatalyst readily oxidized linear- and branched- medium-chain ketones, alkyl levulinates and linear ketones with aromatic substituents with excellent regioselectivity. In addition, this enzyme catalyzed the oxidation of 2-substituted cycloketone derivatives but showed an unusual selection against substituents in positions 3 or 4 of the ring.es
dc.description.filFil: Ceccoli, Romina Denis. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular. Departamento de Ciencias Biológicas; Argentina.es
dc.description.filFil: Ceccoli, Romina Denis. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.filFil: Bianchi, Dario A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR-CONICET); Argentina.es
dc.description.filFil: Fink, Michael J. Harvard University. Department of Chemistry and Chemical Biology; United States or America.es
dc.description.filFil: Fink, Michael J. Vienna University of Technology. Institute of Applied Synthetic Chemistry; Austria.es
dc.description.filFil: Mihovilovic, Marko D. Vienna University of Technology. Institute of Applied Synthetic Chemistry; Austria.es
dc.description.filFil: Rial, Daniela V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular. Departamento de Ciencias Biológicas; Argentina.es
dc.description.filFil: Rial, Daniela V. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PRH 24 PICT 2009-0088es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 11220110101156, PIP 11220150100934)es
dc.description.sponsorshipUniversidad Nacional de Rosario (UNR): BIO287, BIO339es
dc.description.sponsorshipAustrian Science Fund: FWF I723-N17es
dc.description.sponsorshipPrograma de Ayuda para Viajes al Exterior (AVE-UNR)es
dc.formatapplication/pdf
dc.format.extent87es
dc.identifier.issn2191-0855es
dc.identifier.urihttp://hdl.handle.net/2133/19497
dc.language.isoenges
dc.publisherSpringeres
dc.relation.publisherversionhttps://doi.org/10.1186/s13568-017-0390-5es
dc.relation.publisherversionhttps://amb-express.springeropen.com/articles/10.1186/s13568-017-0390-5
dc.rightsopenAccesses
dc.rights.holderCeccoli, Romina Denises
dc.rights.holderBianchi, Dario A.es
dc.rights.holderFink, Michael J.es
dc.rights.holderMihovilovic, Marko D.es
dc.rights.holderRial, Daniela V.es
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectBaeyer–Villiger monooxygenasees
dc.subjectBiocatalysises
dc.subjectLeptospiraes
dc.subjectKetoneses
dc.titleCloning and characterization of the Type I Baeyer–Villiger monooxygenase from Leptospira biflexaes
dc.typepublishedVersion
dc.typearticle
dc.typeartículo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
s13568-017-0390-5.pdf
Tamaño:
2.63 MB
Formato:
Adobe Portable Document Format
Descripción:
Versión publicada
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.59 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Departamento de Ciencias Biológicas - Artículo de Revista