HrpE, the major component of the Xanthomonas type three protein secretion pilus, elicits plant immunity responses

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dc.citation.titleScientific Reports
dc.citation.volume8
dc.creatorGottig, Natalia
dc.creatorVranych, Cecilia Verónica
dc.creatorSgro, Germán Gustavo
dc.creatorPiazza, Ainelén
dc.creatorOttado, Jorgelina
dc.date.accessioned2024-09-11T14:47:22Z
dc.date.available2024-09-11T14:47:22Z
dc.date.issued2018-06-29
dc.description.abstractLike several pathogenic bacteria, Xanthomonas infect host plants through the secretion of effector proteins by the Hrp pilus of the Type Three Protein Secretion System (T3SS). HrpE protein was identified as the major structural component of this pilus. Here, using the Xanthomonas citri subsp. citri (Xcc) HrpE as a model, a novel role for this protein as an elicitor of plant defense responses was found. HrpE triggers defense responses in host and non-host plants revealed by the development of plant lesions, callose deposition, hydrogen peroxide production and increase in the expression levels of genes related to plant defense responses. Moreover, pre-infiltration of citrus or tomato leaves with HrpE impairs later Xanthomonas infections. Particularly, HrpE C-terminal region, conserved among Xanthomonas species, was sufficient to elicit these responses. HrpE was able to interact with plant Glycine-Rich Proteins from citrus (CsGRP) and Arabidopsis (AtGRP-3). Moreover, an Arabidopsis atgrp-3 knockout mutant lost the capacity to respond to HrpE. This work demonstrate that plants can recognize the conserved C-terminal region of the T3SS pilus HrpE protein as a danger signal to defend themselves against Xanthomonas, triggering defense responses that may be mediated by GRPs.
dc.description.filFil: Gottig, Natalia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Vranych, Cecilia Verónica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Sgro, Germán Gustavo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Piazza, Ainelén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ottado, Jorgelina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT2013-0625
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.versionpeerreviewed
dc.format.extent1-13
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/2133/27769
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-018-27869-1
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-018-27869-1
dc.rightsopenAccess
dc.rights.holderGottig, Natalia
dc.rights.holderVranych, Cecilia Verónica
dc.rights.holderSgro, Germán Gustavo
dc.rights.holderPiazza, Ainelén
dc.rights.holderOttado, Jorgelina
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectXanthomonas
dc.subjectInfections
dc.subjectPlants
dc.subjectPlant immunity
dc.subjectHrpE protein
dc.subjectType Three Protein Secretion System (T3SS)
dc.titleHrpE, the major component of the Xanthomonas type three protein secretion pilus, elicits plant immunity responses
dc.typearticulo
dc.type.versionpublishedVersion

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(FBIOyF) Departamento de Microbiología - Artículos
(FBIOyF) Departamento de Química Orgánica - Artículos