Genome-wide plant responses during the non-host interaction of tobacco with the Hemibiotrophic Bacterium Xanthomonas campestris pv. Vesicatoria

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dc.citation.titleFrontiers in Plant Sciencees
dc.citation.volume8es
dc.creatorPierella Karlusich, Juan J.
dc.creatorMatias D. Zurbriggen, Matias D.
dc.creatorShahinnia, Fahimeh
dc.creatorSonnewald, Sophia
dc.creatorSonnewald, Uwe
dc.creatorHosseini, Seyed A.
dc.creatorHajirezaei, Mohammad-Reza
dc.creatorCarrillo, Néstor
dc.date.accessioned2021-02-19T23:05:59Z
dc.date.available2021-02-19T23:05:59Z
dc.date.issued2017-07-04
dc.descriptionNon-host resistance is the most ample and durable form of plant resistance against pathogen infection. It includes induction of defense-associated genes, massive metabolic reprogramming, and in many instances, a form of localized cell death (LCD) at the site of infection, purportedly designed to limit the spread of biotrophic and hemibiotrophic microorganisms. Reactive oxygen species (ROS) have been proposed to act as signals for LCD orchestration. They are produced in various cellular compartments including chloroplasts, mitochondria and apoplast. We have previously reported that down-regulation of ROS build-up in chloroplasts by expression of a plastid-targeted flavodoxin (Fld) suppressed LCD in tobacco leaves inoculated with the non-host bacterium Xanthomonas campestris pv. vesicatoria (Xcv), while other defensive responses were unaffected, suggesting that chloroplast ROS and/or redox status play a major role in the progress of LCD. To better understand these effects, we compare here the transcriptomic alterations caused by Xcv inoculation on leaves of Fld-expressing tobacco plants and their wild-type siblings. About 29% of leaf-expressed genes were affected by Xcv and/or Fld. Surprisingly, 5.8% of them (1,111 genes) were regulated by Fld in the absence of infection, presumably representing pathways responsive to chloroplast ROS production and/or redox status during normal growth conditions. While the majority (∼75%) of pathogen-responsive genes were not affected by Fld, many Xcv responses were exacerbated, attenuated, or regulated in opposite direction by expression of this protein. Particularly interesting was a group of 384 genes displaying Xcv responses that were already triggered by Fld in the absence of infection, suggesting that the transgenic plants had a larger and more diversified suite of constitutive defenses against the attacking microorganism compared to the wild type. Fld modulated many genes involved in pathogenesis, signal transduction, transcriptional regulation and hormone-based pathways. Remarkable interactions with proteasomal protein degradation were observed. The results provide the first genome-wide, comprehensive picture illustrating the relevance of chloroplast redox status in biotic stress responses.es
dc.description.filFil: Pierella Karlusich, Juan J. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Zurbriggen, Matias D. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Shahinnia, Fahimeh. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.es
dc.description.filFil: Sonnewald, Sophia. Friedrich-Alexander-University Erlangen-Nuremberg. Division of Biochemistry. Department of Biology; Germany.es
dc.description.filFil: Sonnewald, Uwe. Friedrich-Alexander-University Erlangen-Nuremberg. Division of Biochemistry. Department of Biology; Germany.es
dc.description.filFil: Hosseini, Seyed A. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.es
dc.description.filFil: Hajirezaei, Mohammad-Reza. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.es
dc.description.filFil: Carrillo, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2012-2851es
dc.description.sponsorshipDeutscher Akademischer Austauschdienst (DAAD)es
dc.description.sponsorshipEuropean Molecular Biology Organization (EMBO)es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)es
dc.description.sponsorshipBunge & Born Foundationes
dc.description.sponsorshipe Leibniz institute of Plant Genetics and Crop Plant Researches
dc.formatapplication/pdf
dc.format.extent1-21es
dc.identifier.issn1664-462Xes
dc.identifier.urihttp://hdl.handle.net/2133/19660
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2017.01158es
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fpls.2017.01158/fulles
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderPierella Karlusich, Juan J.es
dc.rights.holderZurbriggen, Matias D.es
dc.rights.holderShahinnia, Fahimehes
dc.rights.holderSonnewald, Sophiaes
dc.rights.holderSonnewald, Uwees
dc.rights.holderHosseini, Seyed A.es
dc.rights.holderHajirezaei, Mohammad-Rezaes
dc.rights.holderCarrillo, Néstores
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectPlant-microbe Interactionses
dc.subjectChloroplast Redox Statuses
dc.subjectReactive Oxygen Specieses
dc.subjectTranscriptomicses
dc.subjectFlavodoxines
dc.subjectLocalized Cell Deathes
dc.titleGenome-wide plant responses during the non-host interaction of tobacco with the Hemibiotrophic Bacterium Xanthomonas campestris pv. Vesicatoriaes
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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