Light modulates important pathogenic determinants and virulence in ESKAPE pathogens Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus

Vista sencilla de ítem
dc.citation.titleJournal of Bacteriology
dc.citation.volume203
dc.contributor.orcidhttps://orcid.org/0000-0002-9904-7890
dc.contributor.orcidhttps://orcid.org/0000-0002-3339-0100
dc.contributor.orcidhttps://orcid.org/0000-0002-4168-3624
dc.contributor.otherRabinovich, Gabriel: provide HaCaT cells
dc.contributor.otherVoyich, Jovanka: provide the hla and complemented hla mutant
dc.creatorTuttobene, Marisel Romina
dc.creatorPérez, J. F.
dc.creatorPavesi, Estefanía S.
dc.creatorPérez Mora, Bárbara
dc.creatorBiancotti, Daiana
dc.creatorCribb, Pamela
dc.creatorAltilio, Matías
dc.creatorMüller, Gabriela Leticia
dc.creatorGramajo, Hugo Cesar
dc.creatorTamagno, G.
dc.creatorRamírez, María Soledad
dc.creatorDiacovich, Lautaro
dc.creatorMussi, María Alejandra
dc.date.accessioned2024-11-29T12:37:57Z
dc.date.available2024-11-29T12:37:57Z
dc.date.issued2021-02-08
dc.description.abstractLight sensing has been extensively characterized in the human pathogen Acinetobacter baumannii at environmental temperatures. However, the influence of light on the physiology and pathogenicity of human bacterial pathogens at temperatures found in warm-blooded hosts is still poorly understand. In this work, we show that Staphylococcus aureus, Acinetobacter baumannii, and Pseudomonas aeruginosa (ESKAPE) priority pathogens, which have been recognized by the WHO and the CDC as critical, can also sense and respond to light at temperatures found in human hosts. Most interestingly, in these pathogens, light modulates important pathogenicity determinants as well as virulence in an epithelial infection model, which could have implications in human infections. In fact, we found that alpha-toxin-dependent hemolysis, motility, and growth under iron-deprived conditions are modulated by light in S. aureus. Light also regulates persistence, metabolism, and the ability to kill competitors in some of these microorganisms. Finally, light exerts a profound effect on the virulence of these pathogens in an epithelial infection model, although the response is not the same in the different species; virulence was enhanced by light in A. baumannii and S. aureus, while in A. nosocomialis and P. aeruginosa it was reduced. Neither the BlsA photoreceptor nor the type VI secretion system (T6SS) is involved in virulence modulation by light in A. baumannii. Overall, this fundamental knowledge highlights the potential use of light to control pathogen virulence, either directly or by manipulating the light regulatory switch toward the lowest virulence/persistence configuration. IMPORTANCE: Pathogenic bacteria are microorganisms capable of producing disease. Dangerous bacterial pathogens, such as Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii, are responsible for serious intrahospital and community infections in humans. Therapeutics is often complicated due to resistance to multiple antibiotics, rendering them ineffective. In this work, we show that these pathogens sense natural light and respond to it by modulating aspects related to their ability to cause disease; in the presence of light, some of them become more aggressive, while others show an opposite response. Overall, we provide new understanding on the behavior of these pathogens, which could contribute to the control of infections caused by them. Since the response is distributed in diverse pathogens, this notion could prove a general concept.
dc.description.filFil: Tuttobene, Marisel Romina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Argentina.
dc.description.filFil: Pérez, J. F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
dc.description.filFil: Pavesi, Estefanía S. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Argentina.
dc.description.filFil: Pérez Mora, Bárbara. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Argentina.
dc.description.filFil: Biancotti, Daiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Argentina.
dc.description.filFil: Cribb, Pamela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Altilio, Matías. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Argentina.
dc.description.filFil: Müller, Gabriela Leticia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Argentina.
dc.description.filFil: Gramajo, Hugo Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Tamagno, G. Hospital Provincial del Centenario; Argentina.
dc.description.filFil: Ramírez, María Soledad. California State University Fullerton. College of Natural Sciences and Mathematics. Department of Biological Science; United States.
dc.description.filFil: Diacovich, Lautaro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Mussi, María Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 2014-1161
dc.description.sponsorshipAgencia Santafesina de Ciencia Tecnología e Innovación (ASaCTeI): 2010-147-16, IO-021-18
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipInternational Union of Biochemistry and Molecular Biology (IUBMB). Wood-Whelan Research Fellowships
dc.description.versionpeerreviewed
dc.format.extent1-25
dc.identifier.citationTuttobene, M. R., Pérez, J. F., Pavesi, E. S., Pérez Mora, B., Biancotti, D., Cribb, P., Altilio, M., Müller, G. L., Gramajo, H., Tamagno, G., Ramírez, M. S., Diacovich, L. and Mussi, M. A. (2021). Light modulates important pathogenic determinants and virulence in ESKAPE pathogens Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus. Journal of Bacteriology, 203(5). https://doi.org/10.1128/jb.00566-20
dc.identifier.e-issn1098-5530
dc.identifier.issn0021-9193
dc.identifier.urihttps://hdl.handle.net/2133/28259
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.relation.publisherversionhttps://doi.org/10.1128/jb.00566-20
dc.relation.publisherversionhttps://journals.asm.org/doi/10.1128/jb.00566-20
dc.rightsembargoedAccess
dc.rights.holderTuttobene, Marisel Romina
dc.rights.holderPérez, J. F.
dc.rights.holderPavesi, Estefanía S.
dc.rights.holderPérez Mora, Bárbara
dc.rights.holderBiancotti, Daiana
dc.rights.holderCribb, Pamela
dc.rights.holderAltilio, Matías
dc.rights.holderMüller, Gabriela Leticia
dc.rights.holderGramajo, Hugo Cesar
dc.rights.holderTamagno, G.
dc.rights.holderRamírez, María Soledad
dc.rights.holderDiacovich, Lautaro
dc.rights.holderMussi, María Alejandra
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.holderAmerican Society for Microbiology
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectESKAPE pathogen
dc.subjectCell motility
dc.subjectHemolysis
dc.subjectLight regulation
dc.subjectVirulence
dc.subjectAcinetobacter baumannii
dc.subjectPseudomonas aeruginosa
dc.subjectStaphylococcus aureus
dc.titleLight modulates important pathogenic determinants and virulence in ESKAPE pathogens Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus
dc.typearticulo
dc.type.versionpublishedVersion

Archivos

Bloque original
Mostrando 1 - 1 de 1
Nombre:
File - light-modulates-important-pathogenic-determinants-and-virulence-in-eskape-pathogens-acinetobacter.pdf
Tamaño:
5.65 MB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.87 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Departamento de Microbiología - Artículos