Methods: A new protocol for in vitro red blood cell glycation
dc.citation.title | Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology | es |
dc.citation.volume | 264 | es |
dc.creator | Batista da Silva, Marcus V. | |
dc.creator | Alet, Analía I. | |
dc.creator | Castellini, Horacio V. | |
dc.creator | Riquelme, Bibiana Doris | |
dc.date.accessioned | 2021-12-21T16:00:45Z | |
dc.date.available | 2021-12-21T16:00:45Z | |
dc.date.issued | 2021-10-30 | |
dc.description | During diabetes, the characteristic hyperglycemia can induce red blood cell glycation. Several researchers have proposed different protocols to perform an in vitro model to study this phenomenon. In this article, some of the most important in vitro glycation protocols available in the bibliography were compared to each other. The incubation parameters as the suspension medium, glucose concentration, red blood cell concentration, time, and temperature were analyzed. Also, several assays were carried out in our laboratory, and glycated hemoglobin, erythrocyte aggregation and viscoelasticity were determined for the protocol validation. Based on the bibliographic analysis and our experimental results, an optimal protocol for in vitro glycation of red blood cells is presented. | es |
dc.description.fil | Fil: Batista da Silva, Marcus V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. | es |
dc.description.fil | Fil: Alet, Analía I. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. | es |
dc.description.fil | Fil: Castellini, Horacio V. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura. Departamento de Física; Argentina. | es |
dc.description.fil | Fil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. | es |
dc.description.fil | Fil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina. | es |
dc.description.sponsorship | Universidad Nacional de Rosario | es |
dc.description.sponsorship | Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) | es |
dc.format | application/pdf | |
dc.format.extent | 111109 | es |
dc.identifier.issn | 1095-6433 | es |
dc.identifier.uri | http://hdl.handle.net/2133/22972 | |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.cbpa.2021.111109 | es |
dc.rights | embargoedAccess | es |
dc.rights.holder | Elsevier | es |
dc.rights.holder | Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. | es |
dc.rights.holder | Batista da Silva, Marcus V. | es |
dc.rights.holder | Alet, Analía I. | es |
dc.rights.holder | Castellini, Horacio V. | es |
dc.rights.holder | Riquelme, Bibiana Doris | es |
dc.rights.text | Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) | es |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | * |
dc.subject | High blood sugar | es |
dc.subject | Non-enzymatic glycation | es |
dc.subject | In vitro protocol | es |
dc.subject | Glucose incubation | es |
dc.subject | Erythrocyte glycation | es |
dc.title | Methods: A new protocol for in vitro red blood cell glycation | es |
dc.type | article | |
dc.type | artículo | |
dc.type | acceptedVersion | |
dc.type.collection | articulo | |
dc.type.version | acceptedVersion | es |
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