Methods: A new protocol for in vitro red blood cell glycation

dc.citation.titleComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiologyes
dc.citation.volume264es
dc.creatorBatista da Silva, Marcus V.
dc.creatorAlet, Analía I.
dc.creatorCastellini, Horacio V.
dc.creatorRiquelme, Bibiana Doris
dc.date.accessioned2021-12-21T16:00:45Z
dc.date.available2021-12-21T16:00:45Z
dc.date.issued2021-10-30
dc.descriptionDuring diabetes, the characteristic hyperglycemia can induce red blood cell glycation. Several researchers have proposed different protocols to perform an in vitro model to study this phenomenon. In this article, some of the most important in vitro glycation protocols available in the bibliography were compared to each other. The incubation parameters as the suspension medium, glucose concentration, red blood cell concentration, time, and temperature were analyzed. Also, several assays were carried out in our laboratory, and glycated hemoglobin, erythrocyte aggregation and viscoelasticity were determined for the protocol validation. Based on the bibliographic analysis and our experimental results, an optimal protocol for in vitro glycation of red blood cells is presented.es
dc.description.filFil: Batista da Silva, Marcus V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Alet, Analía I. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Castellini, Horacio V. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura. Departamento de Física; Argentina.es
dc.description.filFil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.es
dc.description.sponsorshipUniversidad Nacional de Rosarioes
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)es
dc.formatapplication/pdf
dc.format.extent111109es
dc.identifier.issn1095-6433es
dc.identifier.urihttp://hdl.handle.net/2133/22972
dc.language.isoenges
dc.publisherElsevieres
dc.relation.publisherversionhttps://doi.org/10.1016/j.cbpa.2021.111109es
dc.rightsembargoedAccesses
dc.rights.holderElsevieres
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.es
dc.rights.holderBatista da Silva, Marcus V.es
dc.rights.holderAlet, Analía I.es
dc.rights.holderCastellini, Horacio V.es
dc.rights.holderRiquelme, Bibiana Dorises
dc.rights.textAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es*
dc.subjectHigh blood sugares
dc.subjectNon-enzymatic glycationes
dc.subjectIn vitro protocoles
dc.subjectGlucose incubationes
dc.subjectErythrocyte glycationes
dc.titleMethods: A new protocol for in vitro red blood cell glycationes
dc.typearticle
dc.typeartículo
dc.typeacceptedVersion
dc.type.collectionarticulo
dc.type.versionacceptedVersiones

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