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Methods: A new protocol for in vitro red blood cell glycation

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CBPA METHODS- MANUSCRIPT.pdf (704,52 KB)

Fecha

2021-10-30

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Editor

Elsevier

Resumen

Descripción

During diabetes, the characteristic hyperglycemia can induce red blood cell glycation. Several researchers have proposed different protocols to perform an in vitro model to study this phenomenon. In this article, some of the most important in vitro glycation protocols available in the bibliography were compared to each other. The incubation parameters as the suspension medium, glucose concentration, red blood cell concentration, time, and temperature were analyzed. Also, several assays were carried out in our laboratory, and glycated hemoglobin, erythrocyte aggregation and viscoelasticity were determined for the protocol validation. Based on the bibliographic analysis and our experimental results, an optimal protocol for in vitro glycation of red blood cells is presented.

Palabras clave

High blood sugar, Non-enzymatic glycation, In vitro protocol, Glucose incubation, Erythrocyte glycation

Citación

URI

http://hdl.handle.net/2133/22972

Colecciones

(FBIOyF) Departamento de Química Física - Artículo de Revista

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Licencia Creative Commons

Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
Excepto donde se indique lo contrario, la licencia de este ítem se describe como Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)