Integration of BrfS into the biofilm-controlling cascade promotes sessile Salmonella growth at low temperatures

Vista sencilla de ítem
dc.citation.titleBiofilm
dc.citation.volume9
dc.creatorTulin, Gonzalo
dc.creatorMéndez, Andrea A. E.
dc.creatorFigueroa, Nicolás
dc.creatorSmith, Carol
dc.creatorFolmer, María P.
dc.creatorSerra, Diego Omar
dc.creatorWade, Joseph T.
dc.creatorCheca, Susana Karina
dc.creatorSoncini, Fernando C.
dc.date.accessioned2025-02-14T13:44:38Z
dc.date.available2025-02-14T13:44:38Z
dc.date.issued2025-01-21
dc.description.abstractBiofilm formation is stimulated by different stress-related physiological and environmental conditions. In Salmonella and Escherichia coli, curli fibers and phosphoethanolamine-cellulose are the major extracellular components of biofilms. The production of both is under the control of CsgD, a transcriptional regulator whose expression is modulated by a number of factors responding to different signals. The atypical MerR-like regulator MlrA is key in the activation of csgD transcription in both Salmonella and E. coli. Recently, MlrB, a SPI-2-encoded MlrA-like regulator that counteracts MlrA by repressing csgD transcription and biofilm formation inside mac rophages was identified. Here, we characterize STM1266, a Salmonella-specific MlrA-like regulator, recently renamed BrfS. In contrast to mlrA, brfS transcription increases in minimal growth media and at 20 ◦C, a temperature not commonly tested in laboratories. Under these conditions, as well as in salt-limited rich medium, deletion or overexpression of brfS affects extracellular matrix production. Using transcriptomics, we uncovered genes under BrfS control relevant for biofilm formation such as csgB and bapA. Transcriptional analysis of these genes in mutants lacking brfS, csgD or both, indicates that BrfS controls curli biosynthesis both in a CsgDdependent and independent manner. By contrast, at low temperatures, bapA transcription depends only on BrfS, and neither deletion of csgD nor of mlrA modify its expression. Based on these results, we propose that BrfS contributes to Salmonella persistence in the environment, where the pathogen encounters low temperatures and nutrient limitation.
dc.description.filFil: Tulin, Gonzalo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Méndez, Andrea A. E. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Figueroa, Nicolás. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Figueroa, Nicolás. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI-CONICET); Argentina.
dc.description.filFil: Smith, Carol. Wadsworth Center. New York State Department of Health; United States.
dc.description.filFil: Folmer, María P. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Serra, Diego Omar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Wade, Joseph T. Wadsworth Center. New York State Department of Health; United States.
dc.description.filFil: Wade, Joseph T. University at Albany. School of Public Health. Department of Biomedical Sciences; United States.
dc.description.filFil: Wade, Joseph T. University at Albany. RNA Institute; United States.
dc.description.filFil: Checa, Susana Karina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Soncini, Fernando C. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT-2019-2019-00982; PICT-2018-02122
dc.description.sponsorshipThe National Institutes of Health: 1R35GM144328
dc.description.versionpeerreviewed
dc.format.extent1-9
dc.identifier.citationTulin G, Méndez AAE, Figueroa NR, Smith C, Folmer MP, Serra D, Wade JT, Checa SK, Soncini FC. Integration of BrfS into the biofilm-controlling cascade promotes sessile Salmonella growth at low temperatures. Biofilm. 2025 Jan 21;9:100254. doi: 10.1016/j.bioflm.2025.100254. PMID: 39927094; PMCID: PMC11804604.
dc.identifier.issn2590-2075
dc.identifier.urihttps://hdl.handle.net/2133/28856
dc.language.isoen
dc.publisherElsevier
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S2590207525000024?via%3Dihub
dc.relation.publisherversionhttps://doi.org/10.1016/j.bioflm.2025.100254
dc.rightsopenAccess
dc.rights.holderTulin, Gonzalo
dc.rights.holderMéndez, Andrea A. E.
dc.rights.holderFigueroa, Nicolás
dc.rights.holderSmith, Carol
dc.rights.holderFolmer, María P.
dc.rights.holderSerra, Diego Omar
dc.rights.holderWade, Joseph T.
dc.rights.holderCheca, Susana Karina
dc.rights.holderSoncini, Fernando C.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectSalmonella
dc.subjectBiofilm transcriptional control
dc.subjectMlrA-homolog
dc.subjectExtracellular matrix
dc.subjectLow-temperature
dc.titleIntegration of BrfS into the biofilm-controlling cascade promotes sessile Salmonella growth at low temperatures
dc.typearticulo
dc.type.versionpublishedVersion

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Integration of BrfS into the biofilm-controlling cascade promotes sessile.pdf
Tamaño:
3.99 MB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.87 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Departamento de Microbiología - Artículos