Xanthomonas citri ssp. citri requires the outer membrane porin OprB for maximal virulence and biofilm formation

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dc.citation.titleMolecular Plant Pathology
dc.citation.volume18
dc.creatorFicarra, Florencia Andrea
dc.creatorGrandellis, Carolina
dc.creatorGalván, Estela M.
dc.creatorIelpi, Luis
dc.creatorFeil, Regina
dc.creatorLunn, John E.
dc.creatorGottig, Natalia
dc.creatorOttado, Jorgelina
dc.date.accessioned2024-09-27T13:30:32Z
dc.date.available2024-09-27T13:30:32Z
dc.date.issued2017-06
dc.description.abstractXanthomonas citri ssp. citri (Xcc) causes canker disease in citrus, and biofilm formation is critical for the disease cycle. OprB (Outer membrane protein B) has been shown previously to be more abundant in Xcc biofilms compared with the planktonic state. In this work, we showed that the loss of OprB in an oprB mutant abolishes bacterial biofilm formation and adherence to the host, and also compromises virulence and efficient epiphytic survival of the bacteria. Moreover, the oprB mutant is impaired in bacterial stress resistance. OprB belongs to a family of carbohydrate transport proteins, and the uptake of glucose is decreased in the mutant strain, indicating that OprB transports glucose. Loss of OprB leads to increased production of xanthan exopolysaccharide, and the carbohydrate intermediates of xanthan biosynthesis are also elevated in the mutant. The xanthan produced by the mutant has a higher viscosity and, unlike wild-type xanthan, completely lacks pyruvylation. Overall, these results suggest that Xcc reprogrammes its carbon metabolism when it senses a shortage of glucose input. The participation of OprB in the process of biofilm formation and virulence, as well as in metabolic changes to redirect the carbon flux, is discussed. Our results demonstrate the importance of environmental nutrient supply and glucose uptake via OprB for Xcc virulence.
dc.description.filFil: Ficarra, Florencia Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. (IBR-CONICET); Argentina.
dc.description.filFil: Grandellis, Carolina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. (IBR-CONICET); Argentina.
dc.description.filFil: Galván, Estela M. Fundación Instituto Leloir. Laboratory of Bacterial Genetic. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA-CONICET); Argentina.
dc.description.filFil: Ielpi, Luis. Fundación Instituto Leloir. Laboratory of Bacterial Genetic. Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA-CONICET); Argentina.
dc.description.filFil: Feil, Regina. Wissenschaftspark Potsdam-Golm. Max Planck Institute of Molecular Plant Physiology; Germany.
dc.description.filFil: Lunn, John E. Wissenschaftspark Potsdam-Golm. Max Planck Institute of Molecular Plant Physiology; Germany.
dc.description.filFil: Gottig, Natalia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. (IBR-CONICET); Argentina.
dc.description.filFil: Ottado, Jorgelina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. (IBR-CONICET); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (PICT2013‐0625)
dc.format.extent720-733
dc.identifier.issn1364-3703
dc.identifier.urihttps://hdl.handle.net/2133/27864
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1111/mpp.12433
dc.relation.publisherversionhttps://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/mpp.12433
dc.rightsopenAccess
dc.rights.holderBritish Society for Plant Pathology and John Wiley Online & Sons LTD
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímica y Farmacéuticas
dc.rights.textAttribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectBiofilm
dc.subjectCitrus canker
dc.subjectOprB
dc.subjectXanthomonas citri ssp. citri.
dc.titleXanthomonas citri ssp. citri requires the outer membrane porin OprB for maximal virulence and biofilm formation
dc.typearticulo
dc.type.versionpublishedVersion

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