G-quadruplexes as novel cis-elements controlling transcription during embryonic development

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dc.citation.titleNucleic Acids Research
dc.citation.volume44
dc.creatorDavid, Aldana P.
dc.creatorMargarit, Ezequiel
dc.creatorDomizi, Pablo
dc.creatorBanchio, Claudia
dc.creatorArmas, Pablo
dc.creatorCalcaterra, Nora B.
dc.date.accessioned2024-07-23T17:32:37Z
dc.date.available2024-07-23T17:32:37Z
dc.date.issued2016-01-14
dc.description.abstractG-quadruplexes are dynamic structures folded in G-rich single-stranded DNA regions. These structures have been recognized as a potential nucleic acid based mechanism for regulating multiple cellular processes such as replication, transcription and genomic maintenance. So far, their transcriptional role in vivo during vertebrate embryonic development has not yet been addressed. Here, we performed an in silico search to find conserved putative G-quadruplex sequences (PQSs) within proximal promoter regions of human, mouse and zebrafish developmental genes. Among the PQSs able to fold in vitro as G-quadruplex, those present in nog3, col2a1 and fzd5 promoters were selected for further studies. In cellulo studies revealed that the selected G-quadruplexes affected the transcription of luciferase controlled by the SV40 nonrelated promoter. G-quadruplex disruption in vivo by microinjection in zebrafish embryos of either small ligands or DNA oligonucleotides complementary to the selected PQSs resulted in lower transcription of the targeted genes. Moreover, zebrafish embryos and larvae phenotypes caused by the presence of complementary oligonucleotides fully resembled those ones reported for nog3, col2a1 and fzd5 morphants. To our knowledge, this is the first work revealing in vivo the role of conserved G-quadruplexes in the embryonic development, one of the most regulated processes of the vertebrates biology.
dc.description.filFil: David, Aldana P. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Margarit, Ezequiel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Domizi, Pablo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Banchio, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Armas, Pablo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Calcaterra, Nora B. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica: PICT 2014-0741
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 00773
dc.format.extent4163–4173
dc.identifier.issn1362-4962
dc.identifier.urihttps://hdl.handle.net/2133/27462
dc.language.isoen
dc.publisherOxford University Press
dc.relation.publisherversionhttps://doi.org/10.1093/nar/gkw011
dc.relation.publisherversionhttps://academic.oup.com/nar/article/44/9/4163/2462257?login=true
dc.rightsopenAccess
dc.rights.holderDavid, Aldana P.
dc.rights.holderMargarit, Ezequiel
dc.rights.holderDomizi, Pablo
dc.rights.holderBanchio, Claudia
dc.rights.holderArmas, Pablo
dc.rights.holderCalcaterra, Nora B.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectG-quadruplexes
dc.subjectGene expression regulation
dc.subjectPromoter regions
dc.subjectZebrafish
dc.subjectCell line
dc.titleG-quadruplexes as novel cis-elements controlling transcription during embryonic development
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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