Tumor Necrosis Factor-α Regulates Glucocorticoid Synthesis in the Adrenal Glands of Trypanosoma cruzi Acutely-Infected Mice. The Role of TNF-R1
| dc.contributor.author | Villar, Silvina Raquel | |
| dc.contributor.author | Ronco, María Teresa | |
| dc.contributor.author | Fernández Bussy, Rodrigo | |
| dc.contributor.author | Roggero, Eduardo | |
| dc.contributor.author | Lepletier, Ailin | |
| dc.contributor.author | Manarin, Romina | |
| dc.contributor.author | Savino, Wilson | |
| dc.contributor.author | Pérez, Ana Rosa | |
| dc.contributor.author | Bottasso, Oscar | |
| dc.date.accessioned | 2014-03-26T19:33:41Z | |
| dc.date.available | 2014-03-26T19:33:41Z | |
| dc.date.issued | 2013-05-22 | |
| dc.description.abstract | Adrenal steroidogenesis is under a complex regulation involving extrinsic and intrinsic adrenal factors. TNF-α is an inflammatory cytokine produced in response to tissue injury and several other stimuli. We have previously demonstrated that TNF-R1 knockout (TNF-R1−/−) mice have a dysregulated synthesis of glucocorticoids (GCs) during Trypanosoma cruzi acute infection. Since TNF-α may influence GCs production, not only through the hypothalamus-pituitary axis, but also at the adrenal level, we now investigated the role of this cytokine on the adrenal GCs production. Wild type (WT) and TNF-R1−/− mice undergoing acute infection (Tc-WT and Tc-TNF-R1−/− groups), displayed adrenal hyperplasia together with increased GCs levels. Notably, systemic ACTH remained unchanged in Tc-WT and Tc-TNF-R1−/− compared with uninfected mice, suggesting some degree of ACTH-independence of GCs synthesis. TNF-α expression was increased within the adrenal gland from both infected mouse groups, with Tc-WT mice showing an augmented TNF-R1 expression. Tc-WT mice showed increased levels of P-p38 and P-ERK compared to uninfected WT animals, whereas Tc-TNF-R1−/− mice had increased p38 and JNK phosphorylation respect to Tc-WT mice. Strikingly, adrenal NF-κB and AP-1 activation during infection was blunted in Tc-TNF-R1−/− mice. The accumulation of mRNAs for steroidogenic acute regulatory protein and cytochrome P450 were significantly increased in both Tc-WT and Tc-TNF-R1−/− mice; being much more augmented in the latter group, which also had remarkably increased GCs levels. TNF-α emerges as a potent modulator of steroidogenesis in adrenocortical cells during T. cruzi infection in which MAPK pathways, NF-κB and AP-1 seem to play a role in the adrenal synthesis of pro-inflammatory cytokines and enzymes regulating GCs synthesis. These results suggest the existence of an intrinsic immune-adrenal interaction involved in the dysregulated synthesis of GCs during murine Chagas disease. | es |
| dc.description.affiliation | Fil. Villar, Silvina R. Instituto de Inmunología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario; Argentina | |
| dc.description.affiliation | Fil. Ronco, María Teresa. Instituto de Fisiología Experimental (IFISE-CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario; Argentina | |
| dc.description.peerreviewed | Peer reviewed | es |
| dc.format | application/pdf | |
| dc.identifier.citation | Villar SR, Ronco MT, Ferna´ndez Bussy R, Roggero E, Lepletier A, et al. (2013) Tumor Necrosis Factor-a Regulates Glucocorticoid Synthesis in the Adrenal Glands of Trypanosoma cruzi Acutely-Infected Mice. The Role of TNF-R1. PLoS ONE 8(5): e63814. doi:10.1371/journal.pone.0063814 | es |
| dc.identifier.issn | 1932-6203 | |
| dc.identifier.uri | http://hdl.handle.net/2133/3081 | |
| dc.language.iso | en | es |
| dc.publisher | PLOS (Public Library of Science) | es |
| dc.relation.publisherversion | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0063814 | es |
| dc.rights | openAccess | |
| dc.subject | Trypanosoma cruzi | es |
| dc.subject | Parasitic diseases | es |
| dc.subject | MAPK signaling cascades | es |
| dc.subject | Inflammation | es |
| dc.subject | Adrenal glands | es |
| dc.subject | Cytokines | es |
| dc.subject | Enzyme regulation | es |
| dc.subject | Gene expression | es |
| dc.title | Tumor Necrosis Factor-α Regulates Glucocorticoid Synthesis in the Adrenal Glands of Trypanosoma cruzi Acutely-Infected Mice. The Role of TNF-R1 | es |
| dc.type | artículo | |
| dc.type | article | |
| dc.type | publishedVersion |