Deciphering the evolution of metallo-β-lactamases: A journey from the test tube to the bacterial periplasm

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dc.citation.titleJournal of Biological Chemistryes
dc.citation.volume298
dc.creatorLópez, Carolina
dc.creatorDelmonti, Juliana
dc.creatorBonomo, Robert A.
dc.creatorVila, Alejandro J.
dc.date.accessioned2022-04-07T17:10:39Z
dc.date.available2022-04-07T17:10:39Z
dc.date.issued2022-02-01
dc.descriptionUnderstanding the evolution of metallo-β-lactamases (MBLs) is fundamental to deciphering the mechanistic basis of resistance to carbapenems in pathogenic and opportunistic bacteria. Presently, these MBL-producing pathogens are linked to high rates of morbidity and mortality worldwide. However, the study of the biochemical and biophysical features of MBLs in vitro provides an incomplete picture of their evolutionary potential, since this limited and artificial environment disregards the physiological context where evolution and selection take place. Herein, we describe recent efforts aimed to address the evolutionary traits acquired by different clinical variants of MBLs in conditions mimicking their native environment (the bacterial periplasm) and considering whether they are soluble or membrane-bound proteins. This includes addressing the metal content of MBLs within the cell under zinc starvation conditions and the context provided by different bacterial hosts that result in particular resistance phenotypes. Our analysis highlights recent progress bridging the gap between in vitro and in-cell studies.es
dc.description.filFil: López, Carolina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Metaloproteínas (IBR-CONICET); Argentina.es
dc.description.filFil: Delmonti, Juliana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Metaloproteínas (IBR-CONICET); Argentina.es
dc.description.filFil: Bonomo, Robert A. Veterans Affairs Northeast Ohio Healthcare System. Research Service; United States.es
dc.description.filFil: Bonomo, Robert A. Case Western Reserve University School of Medicine. Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, and Proteomics and Bioinformatics; United States.es
dc.description.filFil: Bonomo, Robert A. Veterans Affairs Northeast Ohio Healthcare System. Medical Service and GRECC; United States.es
dc.description.filFil: Bonomo, Robert A. CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology. U.S. Department of Veterans Affairs; United States.es
dc.description.filFil: Vila, Alejandro J. Instituto de Biología Molecular y Celular de Rosario. Laboratorio de Metaloproteínas (IBR-CONICET); Argentina.es
dc.description.filFil: Vila, Alejandro J. CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology. U.S. Department of Veterans Affairs; United States.es
dc.description.filFil: Vila, Alejandro J. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biofísica; Argentina.es
dc.description.sponsorshipCleveland Department of Veterans Affairs: 1I01BX001974
dc.description.sponsorshipNational Institutes of Health (NIH): R01AI100560
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases (NIAID)
dc.description.sponsorshipBiomedical Laboratory Research and Development, VA Office of Research and Development (BLR&D, ORD)
dc.description.sponsorshipGeriatric Research Education and Clinical Center (GRECC)
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT-2016-1657
dc.description.sponsorshipUniversidad del Rosario (UNR)
dc.formatapplication/pdf
dc.format.extent1-14es
dc.identifier.issn1083-351Xes
dc.identifier.urihttp://hdl.handle.net/2133/23340
dc.language.isoenges
dc.publisherAmerican Society for Biochemistry and Molecular Biology Inc.es
dc.relation.publisherversionhttps://doi.org/10.1016/j.jbc.2022.101665
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0021925822001053?pes=vor
dc.rightsopenAccesses
dc.rights.holderLópez, Carolinaes
dc.rights.holderDelmonti, Julianaes
dc.rights.holderBonomo, Robert A.es
dc.rights.holderVila, Alejandro J.es
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/ar/*
dc.subjectAntibiotic resistancees
dc.subjectMetallo-β-lactamaseses
dc.subjectProtein evolutiones
dc.subjectPeriplasmic spacees
dc.subjectOuter membrane vesicleses
dc.subjectZn(II) limitationes
dc.titleDeciphering the evolution of metallo-β-lactamases: A journey from the test tube to the bacterial periplasmes
dc.typepublishedVersion
dc.typearticle
dc.typeartículo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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