Expression of the agmatine deiminase pathway in Enterococcus faecalis is activated by the AguR regulator and repressed by CcpA and PTS(Man) systems

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dc.citation.titlePLoS ONE
dc.creatorSuárez, Cristian Alejandro
dc.creatorEspariz, Martín
dc.creatorBlancato, Víctor Sebastián
dc.creatorMagni, Christian
dc.date.accessioned2024-08-01T19:04:07Z
dc.date.available2024-08-01T19:04:07Z
dc.date.issued2013-10-14
dc.description.abstractAlthough the agmatine deiminase system (AgDI) has been investigated in Enterococcus faecalis, little information is available with respect to its gene regulation. In this study we demonstrate that the presence of exogenous agmatine induces the expression of agu genes in this bacterium. In contrast to the homologous and extensively characterized AgDI system of S. mutants, the aguBDAC operon in E. faecalis is not induced in response to low pH. In spite of this, agmatine catabolism in this bacterium contributes by neutralizing the external medium while enhancing bacterial growth. Our results indicate that carbon catabolic repression (CCR) operates on the AgDI system via a mechanism that involves interaction of CcpA and PSer-HPr with a cre site found in an unusual position considering the aguB promoter (55 nt upstream the +1 position). In addition, we found that components of the mannose phosphotransferase (PTSMan) system also contributed to CCR in E. faecalis since a complete relief of the PTS-sugars repressive effect was observed only in a PTSMan and CcpA double defective strain. Our gene context analysis revealed that aguR is present in oral and gastrointestinal microorganisms. Thus, regulation of the aguBDAC operon in E. faecalis seems to have evolved to obtain energy and resist low pH conditions in order to persist and colonize gastrointestinal niches.
dc.description.filFil: Suárez, Cristian Alejandro. Instituto de Biología Molecular de Rosario. Laboratorio de Fisiología y Genética de Bacterias Lácticas (IBR-CONICET); Argentina.
dc.description.filFil: Suárez, Cristian Alejandro. Instituto de Biología Molecular de Rosario. Laboratorio de Fisiología y Genética de Bacterias Lácticas (IBR-CONICET); Argentina.
dc.description.filFil: Espariz, Martín. Instituto de Biología Molecular de Rosario. Laboratorio de Fisiología y Genética de Bacterias Lácticas (IBR-CONICET); Argentina.
dc.description.filFil: Espariz, Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: Blancato, Víctor Sebastián. Instituto de Biología Molecular de Rosario. Laboratorio de Fisiología y Genética de Bacterias Lácticas (IBR-CONICET); Argentina.
dc.description.filFil: Blancato, Víctor Sebastián. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: Magni, Christian. Instituto de Biología Molecular de Rosario. Laboratorio de Fisiología y Genética de Bacterias Lácticas (IBR-CONICET); Argentina.
dc.description.filFil: Magni, Christian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT2010-1828, PICT 2008-1562
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP2012-2014
dc.format.extent1-11
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2133/27499
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0076170
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0076170
dc.rightsopenAccess
dc.rights.holderSuárez, Cristian Alejandro
dc.rights.holderEspariz, Martín
dc.rights.holderBlancato, Víctor Sebastián
dc.rights.holderMagni, Christian
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAgmatine
dc.subjectEnterococcus faecalis
dc.subjectGene expression regulation
dc.subjectMolecular sequence data
dc.subjectOperon
dc.titleExpression of the agmatine deiminase pathway in Enterococcus faecalis is activated by the AguR regulator and repressed by CcpA and PTS(Man) systems
dc.typearticulo
dc.type.versionpublishedVersion

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