Cold storage of liver microorgans in ViaSpan® and BG35 solutions. Study of ammonia metabolism during normothermic reoxygenation

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dc.citation.titleAnnals of Hepatology
dc.citation.volume13
dc.creatorPizarro, María Dolores
dc.creatorMediavilla, María Gabriela
dc.creatorBerardi, Florencia
dc.creatorTiribelli, Claudio
dc.creatorRodríguez, Joaquín Valentín
dc.creatorMamprin, María E.
dc.date.accessioned2024-11-04T14:24:34Z
dc.date.available2024-11-04T14:24:34Z
dc.date.issued2014-03
dc.description.abstractIntroduction. This work focuses on ammonia metabolism of Liver Microorgans (LMOs) after cold preservation in a normothermic reoxygenation system (NRS). We have previously reported the development of a novel preservation solution, Bes-Gluconate-PEG 35 kDa (BG35) that showed the same efficacy as ViaSpan® to protect LMOs against cold preservation injury. The objective of this work was to study mRNA levels and activities of two key Urea Cycle enzymes, Carbamyl Phosphate Synthetase I (CPSI) and Ornithine Transcarbamylase (OTC), after preservation of LMOs in BG35 and ViaSpan® and the ability of these tissue slices to detoxify an ammonia overload in a NRS model. Material and methods. After 48 h of cold storage (0°C in BG35 or ViaSpan®) LMOs were rewarmed in KHR containing an ammonium chloride overload (1 mM). We determined ammonium detoxification capacity (ADC), urea synthesis and enzyme activities and relative mRNA levels for CPSI and OTC. Results. At the end of reoxygenation LMOs cold preserved in BG35 have ADC and urea synthesis similar to controls. ViaSpan® group demonstrated a lower capacity to detoxify ammonia and to synthesize urea than fresh LMOs during the whole reoxygenation period which correlated with the lower mRNA levels and activities for CPSI and OTC observed for this group. Conclusion. We demonstrate that our preservation conditions (48 hours, BG35 solution, anoxia, 0ºC) did not affect ammonia metabolism of cold preserved LMOs maintaining the physiological and biochemical liver functions tested, which allows their future use as biological component of a BAL system.
dc.description.filFil: Pizarro, María Dolores. Universidad Nacional de Rosario. Centro Binacional de Criobiología Clínica y Aplicada (CAIC); Argentina.
dc.description.filFil: Mediavilla, María Gabriela. Universidad Nacional de Rosario. Centro Binacional de Criobiología Clínica y Aplicada (CAIC); Argentina.
dc.description.filFil: Berardi, Florencia. Universidad Nacional de Rosario. Centro Binacional de Criobiología Clínica y Aplicada (CAIC); Argentina.
dc.description.filFil: Tiribelli, Claudio. University of Trieste. Trieste and Department of Medical Sc. Centro Studi Fegato; Italy.
dc.description.filFil: Rodríguez, Joaquín Valentín. Universidad Nacional de Rosario. Centro Binacional de Criobiología Clínica y Aplicada (CAIC); Argentina.
dc.description.filFil: Mamprin, María E. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Farmacología; Argentina.
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 112-200801-01208
dc.description.sponsorshipMinistero degli Esteri: Prot. 269/P/0093044, prot. 269/P0114337
dc.description.versionpeerreviewed
dc.format.extent256-264
dc.identifier.citationPizarro, María Dolores; Mediavilla, Maria Gabriela; Berardi, Florencia; Tiribelli, Claudio; Rodriguez, Joaquin Valentin; et al.; Cold Storage of Liver Microorgans in ViaSpan® and BG35 Solutions. Study of Ammonia Metabolism during normothermic reoxygenation; Mexican Assoc Hepatology; Annals Of Hepatology; 13; 2; 3-2014; 256-264
dc.identifier.issn2659-5982
dc.identifier.urihttps://hdl.handle.net/2133/28031
dc.language.isoen
dc.publisherElsevier
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1665268119308890?via%3Dihub
dc.relation.publisherversionhttps://doi.org/10.1016/s1665-2681(19)30889-0
dc.rightsopenAccess
dc.rights.holderFundación Clínica Médica Sur
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 International CC BY-NC-ND 4.0 Deed
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCold storage
dc.subjectPreservation solution
dc.subjectLiver preservation
dc.titleCold storage of liver microorgans in ViaSpan® and BG35 solutions. Study of ammonia metabolism during normothermic reoxygenation
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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