Cerulenin inhibits unsaturated fatty acids synthesis in Bacillus subtilis by modifying the input signal of DesK thermosensor

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dc.citation.titleMicrobiologyOpen
dc.citation.volume3
dc.creatorPorrini, Lucía
dc.creatorCybulski, Larisa Estefanía
dc.creatorAltabe, Silvia Graciela
dc.creatorMansilla, María Cecilia
dc.creatorDe Mendoza, Diego
dc.date.accessioned2024-05-13T18:54:52Z
dc.date.available2024-05-13T18:54:52Z
dc.date.issued2014-04-09
dc.description.abstractBacillus subtilis responds to a sudden decrease in temperature by transiently inducing the expression of the des gene encoding for a lipid desaturase, Δ5-Des, which introduces a double bond into the acyl chain of preexisting membrane phospholipids. This Δ5-Des-mediated membrane remodeling is controlled by the cold-sensor DesK. After cooling, DesK activates the response regulator DesR, which induces transcription of des. We show that inhibition of fatty acid synthesis by the addition of cerulenin, a potent and specific inhibitor of the type II fatty acid synthase, results in increased levels of short-chain fatty acids (FA) in membrane phospholipids that lead to inhibition of the transmembrane-input thermal control of DesK. Furthermore, reduction of phospholipid synthesis by conditional inactivation of the PlsC acyltransferase causes significantly elevated incorporation of long-chain FA and constitutive upregulation of the des gene. Thus, we provide in vivo evidence that the thickness of the hydrophobic core of the lipid bilayer serves as one of the stimulus sensed by the membrane spanning region of DesK.
dc.description.filFil: Porrini, Lucía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Cybulski, Larisa Estefanía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Altabe, Silvia Graciela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Mansilla, María Cecilia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipFondo para la Investigación Científica y Tecnológica (FONCyT)
dc.description.versionpeerreviewed
dc.format.extent1-12
dc.identifier.e-issn2045-8827
dc.identifier.urihttps://hdl.handle.net/2133/27029
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1002/mbo3.154
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1002/mbo3.154
dc.rightsopenAccess
dc.rights.holderPorrini, Lucía
dc.rights.holderCybulski, Larisa Estefanía
dc.rights.holderAltabe, Silvia Graciela
dc.rights.holderMansilla, María Cecilia
dc.rights.holderDe Mendoza, Diego
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBacillus subtilis
dc.subjectCerulenin
dc.subjectThermosensor
dc.subjectUnsaturated fatty acids
dc.titleCerulenin inhibits unsaturated fatty acids synthesis in Bacillus subtilis by modifying the input signal of DesK thermosensor
dc.typearticulo
dc.type.versionpublishedVersion

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