Viral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruses

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dc.citation.volume10
dc.creatorBolatti, Elisa María
dc.creatorViarengo, Gastón
dc.creatorZorec, Tomaž M
dc.creatorCerri, Agustina
dc.creatorMontani, María E.
dc.creatorHošnjak, Lea
dc.creatorCasal, Pablo E.
dc.creatorBortolotto, Eugenia
dc.creatorDi Domenica, Violeta
dc.creatorChouhy, Diego
dc.creatorAllasia, María Belén
dc.creatorBarquez, Rubén M.
dc.creatorPoljak, Mario
dc.creatorGiri, Adriana Angélica
dc.date.accessioned2022-04-08T13:22:33Z
dc.date.available2022-04-08T13:22:33Z
dc.date.issued2022-01-24
dc.descriptionBats are natural reservoirs of a variety of zoonotic viruses, many of which cause severe human diseases. Characterizing viruses of bats inhabiting different geographical regions is important for understanding their viral diversity and for detecting viral spillovers between animal species. Herein, the diversity of DNA viruses of five arthropodophagous bat species from Argentina was investigated using metagenomics. Fecal samples of 29 individuals from five species (Tadarida brasiliensis, Molossus molossus, Eumops bonariensis, Eumops patagonicus, and Eptesicus diminutus) living at two different geographical locations, were investigated. Enriched viral DNA was sequenced using Illumina MiSeq, and the reads were trimmed and filtered using several bioinformatic approaches. The resulting nucleotide sequences were subjected to viral taxonomic classification. In total, 4,520,370 read pairs were sequestered by sequencing, and 21.1% of them mapped to viral taxa. Circoviridae and Genomoviridae were the most prevalent among vertebrate viral families in all bat species included in this study. Samples from the T. brasiliensis colony exhibited lower viral diversity than samples from other species of New World bats. We characterized 35 complete genome sequences of novel viruses. These findings provide new insights into the global diversity of bat viruses in poorly studied species, contributing to prevention of emerging zoonotic diseases and to conservation policies for endangered species.es
dc.description.affiliationFil: Bolatti, Elisa María. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Grupo Virología Humana; Argentina.
dc.description.affiliationFil: Bolatti, Elisa María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.affiliationFil: Viarengo, Gastón. Centro Científico Tecnológico CONICET Rosario. DETx MOL S.A; Argentina.
dc.description.affiliationFil: Zorec, Tomaž M. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia.
dc.description.affiliationFil: Cerri, Agustina. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Grupo Virología Humana; Argentina.
dc.description.affiliationFil: Montani, María E. Museo Provincial de Ciencias Naturales “Dr. Ángel Gallardo”; Argentina.
dc.description.affiliationFil: Montani, María E. Miguel Lillo 251; Argentina.
dc.description.affiliationFil: Montani, María E. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Programa de Investigaciones de Biodiversidad Argentina (PIDBA); Argentina.
dc.description.affiliationFil: Hošnjak, Lea. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia.
dc.description.affiliationFil: Casal, Pablo E. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.affiliationFil: Bortolotto, Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Estadística y Procesamiento de Datos; Argentina.
dc.description.affiliationFil: Di Domenica, Violeta. Miguel Lillo 251; Argentina.
dc.description.affiliationFil: Chouhy, Diego. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Grupo Virología Humana; Argentina.
dc.description.affiliationFil: Chouhy, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.affiliationFil: Chouhy, Diego. Centro Científico Tecnológico CONICET Rosario. DETx MOL S.A; Argentina.
dc.description.affiliationFil: Allasia, María Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Estadística y Procesamiento de Datos; Argentina.
dc.description.affiliationFil: Barquez, Rubén M. Miguel Lillo 251; Argentina.
dc.description.affiliationFil: Barquez, Rubén M. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Instituto Programa de Investigaciones de Biodiversidad Argentina (PIDBA); Argentina.
dc.description.affiliationFil: Poljak, Mario. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia.
dc.description.affiliationFil: Giri, Adriana Angélica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Grupo Virología Humana; Argentina.
dc.description.affiliationFil: Giri, Adriana Angélica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT-2019-01790
dc.description.sponsorshipEuropean Society of Clinical Microbiology and Infectious Diseases (ESCMID)
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipJavna Agencija za Raziskovalno Dejavnost RS (ARRS): P3-0083
dc.formatapplication/pdf
dc.format.extent1-26
dc.identifier.urihttp://hdl.handle.net/2133/23357
dc.language.isoenges
dc.publisherMDPIes
dc.relation.publisherversionhttps://doi.org/10.3390/microorganisms10020266
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/10/2/266
dc.rightsopenAccesses
dc.rights.holderBolatti, Elisa Maríaes
dc.rights.holderViarengo, Gastónes
dc.rights.holderZorec, Tomaž M.es
dc.rights.holderCerri, Agustinaes
dc.rights.holderMontani, María E.es
dc.rights.holderHošnjak, Leaes
dc.rights.holderCasal, Pablo E.es
dc.rights.holderBortolotto, Eugeniaes
dc.rights.holderDi Domenica, Violetaes
dc.rights.holderChouhy, Diegoes
dc.rights.holderAllasia, María Belénes
dc.rights.holderBarquez, Rubén M.es
dc.rights.holderPoljak, Marioes
dc.rights.holderGiri, Adriana Angélicaes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.subjectChiropteraes
dc.subjectMetagenomices
dc.subjectViromees
dc.subjectVirus identificationes
dc.subjectCressdnaviricotaes
dc.subjectCossaviricotaes
dc.subjectAnelloviridaees
dc.titleViral Metagenomic Data Analyses of Five New World Bat Species from Argentina: Identification of 35 Novel DNA Viruseses
dc.typepublishedVersion
dc.typearticle
dc.typeartículo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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