Exploring multitasking proteins in Xanthomonas secretomes: Insights into mechanisms of plant-pathogen interactions

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dc.citation.titleHeliyon
dc.citation.volume11(6)
dc.creatorDe Almeida Barbosa Assis, Renata
dc.creatorPaulino de Oliveira, Amanda Carolina
dc.creatorSouza Carvalho, Flávia Maria
dc.creatorAparecido Ferro, Jesus
dc.creatorDe Souza, Robson Francisco
dc.creatorOrellano, Elena G.
dc.creatorAlmeida, Nalvo Franco
dc.creatorGarcía Machado, Camila Carrião
dc.creatorDandekar, Abhaya M.
dc.creatorChakraborty, Sandeep
dc.creatorVarani, Alessandro M.
dc.creatorMoreira, Leandro Marcio
dc.date.accessioned2025-04-01T16:24:01Z
dc.date.available2025-04-01T16:24:01Z
dc.date.issued2025-03-20
dc.description.abstractRecent advances in large-scale functional genomic analysis have significantly increased interest in multitask proteins. The role of these proteins in Xanthomonas phytopathogens, a model for plantpathogen interaction studies, remains largely underexplored. In this study, we introduce an innovative systematic comparative analysis of secretomes from 18 different Xanthomonas species, integrating data from multiple proteomic studies to identify potential multitasking proteins. This approach led to the identification of 93 proteins primarily involved in central metabolism that are secreted under various physiological conditions, including 16 previously characterized moonlighting proteins. Promiscuity analysis of five selected enzymes revealed that three (asparaginase, chorismate mutase, and phosphoenolpyruvate synthase) exhibit high potential for catalyzing reactions with non-canonical substrates, suggesting additional functional roles beyond their primary enzymatic activities. Additionally, we re-annotated previously hypothetical secreted proteins, assigning functions related to central metabolism and indicating a high potential for promiscuous activity. This comprehensive compilation of potential moonlighting and promiscuous proteins in Xanthomonas provides new insights into the molecular mechanisms driving plantpathogen interactions and establishes a foundation for future experimental validations of these
dc.description.filFil: De Almeida Barbosa Assis, Renata. University of California. Department of Plant Sciences; United States.
dc.description.filFil: De Almeida Barbosa Assis, Renata. Federal University of Ouro Preto. Center of Research in Biological Science; Brazil.
dc.description.filFil: Marini Ferreira, Rafael. Universidade Estadual Paulista. Faculty of Agricultural and Veterinary Sciences of Jaboticabal. Department of Agricultural and Environmental Biotechnology, Jaboticabal; Brazil.
dc.description.filFil: Paulino de Oliveira, Amanda Carolina. Universidade Estadual Paulista. Faculty of Agricultural and Veterinary Sciences of Jaboticabal. Department of Agricultural and Environmental Biotechnology, Jaboticabal; Brazil.
dc.description.filFil: Souza Carvalho, Flávia Maria. Universidade Estadual Paulista. Faculty of Agricultural and Veterinary Sciences of Jaboticabal. Department of Agricultural and Environmental Biotechnology, Jaboticabal; Brazil.
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Almeida, Nalvo Franco. Universidade Federal de Mato Grosso do Sul. Faculdade de Computaçao; Brazil.
dc.description.filFil: García Machado, Camila Carrião. Federal University of Ouro Preto. Center of Research in Biological Science; Brazil.
dc.description.filFil: García Machado, Camila Carrião. Federal University of Ouro Preto. Institute of Exact and Biological Science. Department of Biological Science and Center of Research in Biological Science; Brazil.
dc.description.filFil: Dandekar, Abhaya M. University of California. Department of Plant Sciences; United States.
dc.description.filFil: Chakraborty, Sandeep. Independent Researcher; India.
dc.description.filFil: Varani, Alessandro M. Universidade Estadual Paulista. Faculty of Agricultural and Veterinary Sciences of Jaboticabal. Department of Agricultural and Environmental Biotechnology, Jaboticabal; Brazil.
dc.description.filFil: Moreira, Leandro Marcio. Federal University of Ouro Preto. Center of Research in Biological Science; Brazil.
dc.description.filFil: Moreira, Leandro Marcio. Federal University of Ouro Preto. Institute of Exact and Biological Science. Department of Biological Science and Center of Research in Biological Science; Brazil.
dc.format.extent1-23
dc.identifier.citationAssis, R. de AB, Ferreira, RM, de Oliveira, ACP, Carvalho, FMS, Ferro, JA, de Souza, RF, Orellano, EG, Almeida, NF, García, CCM, Dandekar, AM, Chakraborty, S., Varani, AM y Moreira, LM (2025). Exploración de proteínas multitarea en secretomas de Xanthomonas: conocimientos sobre los mecanismos de las interacciones entre plantas y patógenos. Heliyon, 11(6), e42979. https://doi.org/10.1016/j.heliyon.2025.e42979
dc.identifier.issn2405-8440
dc.identifier.urihttps://hdl.handle.net/2133/29151
dc.language.isoen
dc.publisherElsevier
dc.relation.publisherversionhttps://doi.org/10.1016/j.heliyon.2025.e42979
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S240584402501360X?via%3Dihub
dc.rightsopenAccess
dc.rights.holderDe Almeida Barbosa Assis, Renata
dc.rights.holderMarini Ferreira, Rafael
dc.rights.holderPaulino de Oliveira, Amanda Carolina
dc.rights.holderSouza Carvalho, Flávia Maria
dc.rights.holderAparecido Ferro, Jesus
dc.rights.holderDe Souza, Robson Francisco
dc.rights.holderOrellano, Elena G.
dc.rights.holderAlmeida, Nalvo Franco
dc.rights.holderGarcía Machado, Camila Carrião
dc.rights.holderDandekar, Abhaya M.
dc.rights.holderChakraborty, Sandeep
dc.rights.holderVarani, Alessandro M.
dc.rights.holderMoreira, Leandro Marcio
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPlant-pathogen interactions
dc.subjectMoonlight proteins
dc.subjectPromiscuous proteins
dc.subjectMultitask proteins
dc.subjectComparative secretome
dc.subjectAdaptation
dc.titleExploring multitasking proteins in Xanthomonas secretomes: Insights into mechanisms of plant-pathogen interactions
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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