Anatomical and biochemical changes induced by Gluconacetobacter diazotrophicus stand up for Arabidopsis thaliana seedlings from Ralstonia solanacearum infection

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dc.citation.titleFrontiers in Plant Sciencees
dc.citation.volume10es
dc.creatorRodriguez, María V.
dc.creatorTano, Josefina
dc.creatorAnsaldi, Nazarena
dc.creatorCarrau, Analía
dc.creatorSrebot, María S.
dc.creatorFerreira, Virginia
dc.creatorMartínez, María Laura
dc.creatorCortadi, Adriana A.
dc.creatorSiri, María I.
dc.creatorOrellano, Elena G.
dc.date.accessioned2020-12-21T21:24:54Z
dc.date.available2020-12-21T21:24:54Z
dc.date.issued2019-12-23
dc.descriptionNowadays, fertilization and pest control are carried out using chemical compounds that contaminate soil and deteriorate human health. Plant growth promoting bacteria endophytes (PGPBEs), are a well-studied group of bacteria that offers benefits to the host plant, such as phytostimulation, biofertilization, and protection against other microorganisms. The study of Gluconacetobacter diazotrophicus–which belongs to PGPBEs-aids the development of alternative strategies of an integrated approach for crop management practices. Ralstonia solanacearum is responsible for bacterial wilt disease. This phytopathogen is of great interest worldwide due to the enormous economic losses it causes. In this study the action of G. diazotrophicus as a growth promoting bacterium in Arabidopsis thaliana seedlings is analyzed, evaluating the antagonistic mechanisms of this beneficial endophytic bacterium during biotic stress produced by R. solanacearum. Effective colonization of G. diazotrophicus was determined through bacterial counting assays, evaluation of anatomical and growth parameters, and pigments quantification. Biocontrol assays were carried out with Ralstonia pseudosolanacearum GMI1000 model strain and R. solanacearum A21 a recently isolated strain. Inoculation of A. thaliana (Col 0) with G. diazotrophicus Pal 5 triggers a set of biochemical and structural changes in roots, stems, and leaves of seedlings. Discrete callose deposits as papillae were observed at specific sites of root hairs, trichomes, and leaf tissue. Upon R. pseudosolanacearum GMI1000 infection, endophyte-treated plants demonstrated being induced for defense through an augmented callose deposition at root hairs and leaves compared with the non-endophyte-treated controls. The endophytic bacterium appears to be able to prime callose response. Roots and stems cross sections showed that integrity of all tissues was preserved in endophyte-treated plants infected with R. solanacearum A21. The mechanisms of resistance elicited by the plant after inoculation with the endophyte would be greater lignification and sclerosis in tissues and reinforcement of the cell wall through the deposition of callose. As a consequence of this priming in plant defense response, viable phytopathogenic bacteria counting were considerably fewer in endophyte-inoculated plants than in not-inoculated controls. Our results indicate that G. diazotrophicus colonizes A. thaliana plants performing a protective role against the phytopathogenic bacterium R. solanacearum promoting the activation of plant defense system.es
dc.description.filFil: Rodriguez, María V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Vegetal; Argentina.es
dc.description.filFil: Rodriguez, María V. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.filFil: Tano, Josefina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Ansaldi, Nazarena. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Vegetal; Argentina.es
dc.description.filFil: Ansaldi, Nazarena. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.filFil: Carrau, Analía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Srebot, María S. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Vegetal; Argentina.es
dc.description.filFil: Srebot, María S. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.filFil: Ferreira, Virginia. Universidad de la República. Facultad de Química. Departamento de Biociencias; Uruguay.es
dc.description.filFil: Martínez, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Vegetal; Argentina.es
dc.description.filFil: Martínez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.filFil: Cortadi, Adriana A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Vegetal; Argentina.es
dc.description.filFil: Cortadi, Adriana A. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.filFil: Siri, María I. Universidad de la República. Facultad de Química. Departamento de Biociencias; Uruguay.es
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular; Argentina.es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)es
dc.description.sponsorshipUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.es
dc.formatapplication/pdf
dc.format.extent1618es
dc.identifier.issn1664-462Xes
dc.identifier.urihttp://hdl.handle.net/2133/19530
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fpls.2019.01618/fulles
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2019.01618es
dc.rightsopenAccesses
dc.rights.holderTano, Josefinaes
dc.rights.holderAnsaldi, Nazarenaes
dc.rights.holderCarrau, Analíaes
dc.rights.holderSrebot, María S.es
dc.rights.holderFerreira, Virginiaes
dc.rights.holderMartínez, María Lauraes
dc.rights.holderCortadi, Adriana A.es
dc.rights.holderSiri, María I.es
dc.rights.holderOrellano, Elena G.es
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.textAtribución 4.0 Internacional (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es*
dc.subjectBiocontroles
dc.subjectGluconacetobacter diazotrophicuses
dc.subjectInduced systemic resistancees
dc.subjectPlant growth promoting bacteria endophytees
dc.subjectRalstonia solanacearumes
dc.titleAnatomical and biochemical changes induced by Gluconacetobacter diazotrophicus stand up for Arabidopsis thaliana seedlings from Ralstonia solanacearum infectiones
dc.typepublishedVersion
dc.typearticle
dc.typeartículo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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