Assessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRs

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dc.citation.titleGeneses
dc.citation.volume10(9)es
dc.creatorMastrochirico-Filho, Vito Antonio
dc.creatorDel Pazo, Felipe
dc.creatorHata, Milene Elissa
dc.creatorVillanova, Gabriela Vanina
dc.creatorForesti, Fausto
dc.creatorVera, Manuel
dc.creatorMartínez, Paulino
dc.creatorPorto-Foresti, Fábio
dc.creatorHashimoto, Diogo Teruo
dc.date.accessioned2021-03-19T15:18:59Z
dc.date.available2021-03-19T15:18:59Z
dc.date.issued2019-08-31
dc.descriptionThe pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their applications in breeding programs. The aim of this study was to characterize the genetic diversity of seven farmed populations of pacu which will constitute the basis for a broodstock foundation for coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a reference to compare genetic parameters in the farmed populations. The analyses were performed using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers. No significant differences in genetic diversity between populations estimated through the number of alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the wild population. Moreover, we detected low genetic structure when comparing farmed and wild populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance (AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis showed that most fish farms included related individuals at a proportion of at least 25%. Our results suggest that the basal broodstock for pacu breeding programs should be founded with individuals from different fish farms for higher genetic diversity and to avoid inbreeding risks.es
dc.descriptionPara citar este articulo: Mastrochirico-Filho, V.A.; del Pazo, F.; Hata, M.E.; Villanova, G.V.; Foresti, F.; Vera, M.; Martínez, P.; Porto-Foresti, F.; Hashimoto, D.T. Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs. Genes 2019, 10, 668. https://doi.org/10.3390/genes10090668
dc.description.filFil: Mastrochirico-Filho, Vito Antonio. São Paulo State University (Unesp). Unesp's Aquaculture Center (Caunesp); Brazil.es
dc.description.filFil: Del Pazo, Felipe. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio Mixto de Biotecnología Acuática (LMBA - UNR - Ministerio de Ciencia, Tecnología e Innovación productiva de Santa Fe); Argentina.es
dc.description.filFil: Del Pazo, Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.filFil: Hata, Milene Elissa. São Paulo State University (Unesp). Unesp's Aquaculture Center (Caunesp); Brazil.es
dc.description.filFil: Villanova, Gabriela Vanina. Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio Mixto de Biotecnología Acuática (LMBA - UNR - Ministerio de Ciencia, Tecnología e Innovación productiva de Santa Fe); Argentina.es
dc.description.filFil: Villanova, Gabriela Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.filFil: Foresti, Fausto. São Paulo State University (Unesp). Institute of Biosciences; Brazil.es
dc.description.filFil: Vera, Manuel. Universidad de Santiago de Compostela (USC). Facultad de Veterinaria. España.es
dc.description.filFil: Vera, Manuel. Universidad de Santiago de Compostela (USC). Instituto de Acuicultura; España.es
dc.description.filFil: Martínez, Paulino. Universidad de Santiago de Compostela (USC). Facultad de Veterinaria. España.es
dc.description.filFil: Martínez, Paulino. Universidad de Santiago de Compostela (USC). Instituto de Acuicultura; España.es
dc.description.filFil: Porto-Foresti, Fábio. São Paulo State University (Unesp). School of Sciences; Brazil.es
dc.description.filFil: Hashimoto, Diogo Teruo. São Paulo State University (Unesp). Unesp's Aquaculture Center (Caunesp); Brazil.es
dc.description.sponsorshipPró-Reitoria de Pesquisa da UNESP: Prope 07/2015es
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico: CNPq 446779/2014-8, 305916/2015-7 y 130262/2014-5es
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo: FAPESP 2014/03772-7es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT) – Gobierno de Santa Fe: PID 020-2013es
dc.formatapplication/pdf
dc.format.extent1-13es
dc.identifier.issn2073-4425es
dc.identifier.urihttp://hdl.handle.net/2133/20241
dc.language.isoenges
dc.publisherMDPIes
dc.relation.publisherversionhttps://doi.org/10.3390/genes10090668es
dc.relation.publisherversionhttps://www.mdpi.com/2073-4425/10/9/668es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderMastrochirico-Filho, Vito Antonioes
dc.rights.holderDel Pazo, Felipees
dc.rights.holderHata, Milene Elissaes
dc.rights.holderVillanova, Gabriela Vaninaes
dc.rights.holderForesti, Faustoes
dc.rights.holderVera, Manueles
dc.rights.holderMartínez, Paulinoes
dc.rights.holderPorto-Foresti, Fábioes
dc.rights.holderHashimoto, Diogo Teruoes
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectAquaculturees
dc.subjectGenetic Variabilityes
dc.subjectInbreedinges
dc.subjectBreeding Programses
dc.titleAssessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRses
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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