Assessing genetic diversity for a pre-breeding program in Piaractus mesopotamicus by SNPs and SSRs
Cargando...
Archivos
Fecha
Título de la revista
ISSN de la revista
Título del volumen
Editor
MDPI
Resumen
Descripción
The pacu (Piaractus mesopotamicus) is a Neotropical fish with remarkable productive
performance for aquaculture. Knowledge of genetic resources in Neotropical fish is essential for their
applications in breeding programs. The aim of this study was to characterize the genetic diversity of
seven farmed populations of pacu which will constitute the basis for a broodstock foundation for
coming breeding programs in Brazil. Analysis of one wild population (Paraná River) was used as a
reference to compare genetic parameters in the farmed populations. The analyses were performed
using 32 single-nucleotide polymorphisms (SNP) and 8 simple sequence repeat (SSR) markers.
No significant differences in genetic diversity between populations estimated through the number of
alleles and allelic richness, observed heterozygosity, expected heterozygosity, and minimum allele
frequency were detected (p > 0.05). Low genetic diversity was observed in all farmed stocks and the
wild population. Moreover, we detected low genetic structure when comparing farmed and wild
populations for SNPs (FST = 0.07; K = 3) and SSRs (FST = 0.08; K = 2). Analysis of molecular variance
(AMOVA) demonstrated that genetic variation was mostly within populations. Kinship analysis
showed that most fish farms included related individuals at a proportion of at least 25%. Our results
suggest that the basal broodstock for pacu breeding programs should be founded with individuals
from different fish farms for higher genetic diversity and to avoid inbreeding risks.
Para citar este articulo: Mastrochirico-Filho, V.A.; del Pazo, F.; Hata, M.E.; Villanova, G.V.; Foresti, F.; Vera, M.; Martínez, P.; Porto-Foresti, F.; Hashimoto, D.T. Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs. Genes 2019, 10, 668. https://doi.org/10.3390/genes10090668
Para citar este articulo: Mastrochirico-Filho, V.A.; del Pazo, F.; Hata, M.E.; Villanova, G.V.; Foresti, F.; Vera, M.; Martínez, P.; Porto-Foresti, F.; Hashimoto, D.T. Assessing Genetic Diversity for a Pre-Breeding Program in Piaractus mesopotamicus by SNPs and SSRs. Genes 2019, 10, 668. https://doi.org/10.3390/genes10090668
Palabras clave
Citación
Aprobación
Revisión
Complementado por
Referenciado por
Licencia Creative Commons
Excepto donde se indique lo contrario, la licencia de este ítem se describe como Attribution 4.0 International (CC BY 4.0)